Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr. by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus)

Tree Physiology

Volumn 30, Issue 12, 2010, Pages 1599-1605

  Jia, Zhichun ^a   Gou, Jiqing ^b   Sun, Yimin ^a   Yuan, Li ^a   Tang, Qiao ^a   Yang, Xingyong ^a   Pei, Yan ^a   Luo, Keming ^a  

^a SOUTHWEST UNIVERSITY   (China)

^b MICHIGAN TECHNOLOGICAL UNIVERSITY   (United States)

Author keywords

antimicrobial protein; fungal resistance; Populus tomentosa Carr.; transgenic plant

Indexed keywords

ANTIMICROBIAL ACTIVITY; BIOASSAY; DECIDUOUS TREE; DISEASE RESISTANCE; FUNGUS; GENE EXPRESSION; GENETIC ANALYSIS; HERB; LIPID; POLYMERASE CHAIN REACTION; PROTEIN; TRANSGENIC PLANT;

ARTICLE; ASPEN; GENETIC PREDISPOSITION; GENETICS; IMMUNOLOGY; LEONURUS; METABOLISM; MICROBIOLOGY; PLANT DISEASE; PLANT LEAF; TRANSGENIC PLANT;

GENETIC PREDISPOSITION TO DISEASE; LEONURUS; PLANT DISEASES; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; POPULUS;

AGROBACTERIUM; ALTERNARIA ALTERNATA; GLOMERELLA CINGULATA; LEONURUS; LEONURUS JAPONICUS; POPULUS TOMENTOSA;

EID: 78649623049     PISSN: 0829318X     EISSN: 17584469     Source Type: Journal    
DOI: 10.1093/treephys/tpq093     Document Type: Article

References (48)

1. Ahuja, M.R. 1987. Gene transfer in forest trees. In Genetic Manipulation of Woody Plants. Eds. J.W. Hanover and D.E. Keathley. Plenum Press, New York, pp 25-42.
2. Blein, J.P., P. Coutos-Thevenotb, D. Marionc and M. Ponchet. 2002. From elicitins to lipid-transfer proteins: a new insight in cell signaling involved in plant defence mechanisms. Trends Plant Sci. 7:293-296.
3. Brogue, K., I. Chet, M. Holliday, R. Cressman, P. Biddle, S. Knowlton, C.J. Mauvais and R. Broglie. 1991. Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254:1194-1197.
4. Carvalho, A.O. and V.M. Gomes. 2007. Role of plant lipid transfer proteins in plant cell physiology - a concise review. Peptides 28:1144-1153.
5. Cary, J.W., K. Rajasekaran, J.M. Jaynes and T.E. Cleveland. 2000. Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta. Plant Sci. 154:171-181. (Pubitemid 30350908)
6. Cellerino, G.P. 1999. Review of fungal diseases in poplar. Food and Agriculture Organization of the United Nations. Rome, 1999. AC492/E.
7. Chen, W.P. and Z.K. Punja. 2002. Transgenic herbicide and disease tolerant carrot (Daucus carota L.) plants obtained through Agrobacterium- mediated transformation. Plant Cell Rep. 20:929-935.
8. Cheng, C.S., D. Samuel, Y.J. Liu, J.C. Shyu, S.M. Lai, K.F. Lin and P.C. Lyu. 2004. Binding mechanism of non-specific lipid transfer proteins and their role in plant defense. Biochemistry 43:13628-13636.
9. Garcia-Olmedo, F., A. Molina, J.M. Alamillo and P. Rodriguez-Palenzuela. 1998. Plant defense peptides. Biopolymers 47:479-491.
10. Gomes, E., E. Sagot, C. Gaillard, L. Laquitaine, B. Poinssot, Y.H. Sanejouand, S. Delrot and P. Coutos-Thevenot. 2003. Nonspecific lipid-transfer protein genes expression in grape (Vitis sp.) cells in response to fungal elicitor treatments. Mol. Plant- Microbe Interact. 16:456-464.
11. Hancock, R.E. and R. Lehrer. 1998. Cationic peptides: a new source of antibiotics. Trends Biotechnol. 16:82-88.
12. Huang, Y., R.O. Nordeen, M. Di, L.D. Owens and J.H. McBeath. 1997. Expression of an engineered cecropin gene cassette in transgenic tobacco plants confers disease resistance to Pseudomonas syringae pv. tabaci. Phytopathology 87: 494-499.
13. Jayaraj, J., A. Anand and S. Muthukrishnan. 2004. Pathogenesis-related proteins and their roles in resistance to fungal pathogens. In Fungal Disease Resistance in Plants - Biochemistry, Molecular Biology and Genetic Engineering. Ed. Z.K. Punja. Food Products Press, New York, pp 139-178.
14. Jia, Z.C., Y.M. Sun, L. Yuan, Q.Y. Tian and K.M. Luo. 2010. The chitinase gene (Bbchit1) from Beauveria bassiana enhances resistance to Cytospora chrysosperma in Populus tomentosa Carr. Biotechnol. Lett. 32:1325-1332.
15. Jung, H.W., K.D. Kim and B.K. Hwang. 2005. Identification of pathogen-responsive regions in the promoter of a pepper lipid transfer protein gene (CALTPI) and the enhanced resistance of the CALTPI transgenic Arabidopsis against pathogen and environmental stresses. Planta 221:361-373.
16. Klopfenstein, N.B., Y.W. Chun, M.S. Kim and M.R. Ahuja. 1997. Micropropagation, genetic engineering, and molecular biology of Populus. USDA For. Serv., Rocky Mountain Forest and Range Exp. Stn., Fort Collins, CO, Gen. Tech. Rep. RM-297, 326 p.
17. Li, Q., C.B. Lawrence, H.Y. Xing, R.A. Babbitt, W.T. Bass, I.B. Maiti and N.P. Everett. 2001. Enhanced disease resistance conferred by expression of an antimicrobial magainin analog in transgenic tobacco. Planta 212:635-639.
18. Li, X., K. Gasic, B.P.A. Cammue, W.F. Broekaert and S.S. Korban. 2003. Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1, demonstrate enhanced resistance to powdery mildew (Sphaerotheca pannosa). Planta 218:226-232.
19. Lin, K.F., Y.N. Liu, S.T.D. Hsu, D. Samue, C.S. Cheng, A.M.J.J. Bonvin and P.C. Lyu. 2005. Characterization and structural analyses of non-specific lipid transfer protein 1 from mung bean. Biochemistry 44:5703-5712.
20. Lloyd, G. and B. McCown. 1980. Commercially-feasible micropro-pagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb. Proc. Int. Plant Prop. Soc. 30:421-427.
21. Lorito, M., S.L. Woo, I. Garcia, G. Colucci, G.E. Harman, J.A. Pintor-Toro, E. Filippone, S. Muccifora, C.B. Lawrence, A. Zoina, S. Tuzun and F. Scala. 1998. Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens. Proc. Natl Acad. Sci. USA 95:7860-7865.
22. Luo, K.M., X.L. Zheng, Y.Q. Chen, Y.H. Xiao, D.G. Zhao, R. McAvoy, Y. Pei and Y. Li. 2006. The maize Knotted1 gene as a positive selectable marker gene is effective for Agrobacterium-mediated transformation in tobacco. Plant Cell Rep. 25:403-409.
23. Makandar, R., J.S. Essig, M.A. Schapaugh, H.N. Trick and J. Shah. 2006. Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1. Mol. Plant- Microbe Interact. 19:123-129.
24. Maldonado, A.M., P. Doerner, R.A. Dixon, C.J. Lamb and R.K. Cameron. 2002. A putative lipid transfer protein involved in systemic resistance signalling in Arabidopsis. Nature 419: 399-403.
25. Melchers, L.S. and M.H. Stuiver. 2000. Novel genes for disease resistance breeding. Curr. Opin. Plant Biol. 3:147-152.
26. Molina, A. and F. Garcia-Olmedo. 1997. Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2. Plant J. 12:669-675.
27. Oh, I.S., A.R. Park, M.S. Bae, S.J. Kwon, Y.S. Kim, J.E. Lee, N. Y. Kang, S. Lee, H. Cheong and O.K. Park. 2005. Secretome analysis reveals an Arabidopsis lipase involved in defense against Alternaria brassicicola. Plant Cell 17:2832-2847.
28. Ostry, M.E. and H.S. McNabb Jr. 1983. Diseases of intensively cultured hybrid poplars: a summary of recent research in the north central region. Gen. Tech. Rep. North Centr. For. Exp. Station 91:102-109.
29. Punja, Z.K. 2005. Transgenic carrots expressing a thaumatin-like protein display enhanced resistance to several fungal pathogens. Can. J. Plant Pathol. 27:291-296.
30. Punja, Z.K. 2006. Recent developments toward achieving fungal disease resistance in transgenic plants. Can. J. Plant Pathol. 28: S298-308.
31. Punja, Z.K. and S.H.T. Raharjo. 1996. Response of transgenic cucumber and carrot plants expressing different chitinase enzymes to inoculation with fungal pathogens. Plant Dis. 80:999-1005.
32. Rao, A.G. 1995. Antimicrobial peptides. Mol. Plant-Microbe Interact. 8:6-13.
33. Roy-Barman, S., C. Sautter and B.B. Chattoo. 2006. Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses. Transgenic Res. 15:435-446.
34. Samuel, D., Y.J. Liu, C.S. Cheng and P.C. Lyu. 2002. Solution structure of plant non-specific lipid transfer protein-2 from rice (Oryza sativa). J. Biol. Chem. 277:35267-35273.
35. Schlumbaum, A.F., F. Mauch, U. Vögeli and T. Boller. 1986. Plant chitinases are potent inhibitors of fungal growth. Nature 324:365-367.
36. Selitrennikoff, C.P. 2001. Antifungal proteins. Appl. Environ. Microbiol. 67:2883-2894.
37. Spelbrink, R.G., N. Dilmac, A. Allen, T.J. Smith, D.M. Shah and G.H. Hockerman. 2004. Differential antifungal and calcium channel-blocking activity among structurally related plant defensins. Plant Physiol. 135:2055-2067.
38. Stettler, R.F., H.D. Bradshaw, P.E. Heilman Jr and T.M. Hinckley. 1996. Biology of Populus and its implications for management and conservation. NRC Research Press, Ottawa, ON, 539 p.
39. Sun, J.Y., D.A. Gaudet, Z.X. Lu, M. Frick, B. Puchalski and A. Laroche. 2008. Characterization and antifungal properties of wheat non-specific lipid transfer proteins. Mol. Plant-Microbe. Interact. 21:346-360.
40. Takaichi, M. and K. Oeda. 2000. Transgenic carrots with enhanced resistance against two major pathogens, Erysiphe heraclei and Alternaria dauci. Plant Sci. 153:135-144.
41. Terras, F.R.G., K. Eggermont, V. Kovaleva et al. 1995. Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7:573-588.
42. Tomassen, M.M., D.M. Barret, H.C. van der Valk and E.J. Woltering. 2007. Isolation and characterization of a tomato nonspecific lipid transfer protein involved in polygalacturonase mediated pectin degradation. J. Exp. Bot. 58:1151-1160.
43. Wang, C., W. Xie, F. Chi, W. Hu, G. Mao, D. Sun, C. Li and Y. Sun. 2007. BcLTP, a novel lipid transfer protein in Brassica chinensis, may secrete and combine extracellular CaM. Plant Cell Rep. 27:159-169.
44. Yang, D., L. Bernier and M. Dessureault. 1994. Biological control of Septoria leaf spot of poplar by Phaeotheca dimorphospora. Plant Dis. 78:821-825.
45. Yang, X.Y., J. Li, X.B. Li, R. She and Y. Pei. 2006. Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds. Peptides 27:3122-3128.
46. Yang, X.Y., X.W. Wang, X.B. Li, B.B. Zhang, Y.H. Xiao, D.M. Li, C.J. Xie and Y. Pei. 2008. Characterization and expression of an nsLTPs-like antimicrobial protein gene from motherwort (Leonurus japonicus). Plant Cell Rep. 27:759-766.
47. Zasloff, M. 2002. Antimicrobial peptides of multicellular organisms. Nature 415:389-395.
48. Zhang, X.D., Y. Dai, Y.Q. Xiong, C. DeFraia, J.Y. Li, X.N. Dong and Z.L. Mou. 2007. Overexpression of Arabidopsis MAP kinase kinase 7 leads to activation of plant basal and systemic acquired resistance. Plant J. 52:1066-1079.