Functional analysis of two isoforms of phosphatidylethanolamine N-methyltransferase

Biochemical Journal

Volumn 432, Issue 2, 2010, Pages 387-398

  Morita, Shin Ya ^a   Takeuchi, Atsuko ^a   Kitagawa, Shuji ^a  

^a KOBE PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

Electrospray ionization tandem MS (ESI MS MS); Enzymatic measurement; Phosphatidylcholine (PC); Phosphatidylethanolamine (PE); Phosphatidylethanolamine N methyltransferase (PEMT); Phosphatidylserine (PS)

Indexed keywords

ENZYMATIC MEASUREMENT; PHOSPHATIDYLCHOLINE (PC); PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE; PHOSPHATIDYLSERINE; TANDEM MS;

CELL CULTURE; ETHERS; IONIZATION; PARTICLE DETECTORS;

ELECTROSPRAY IONIZATION;

ISOPROTEIN; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLETHANOLAMINE METHYLTRANSFERASE; PHOSPHATIDYLSERINE;

ARTICLE; CELL PROLIFERATION; CELLULAR DISTRIBUTION; COMPARATIVE STUDY; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME SPECIFICITY; GLYCOSYLATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

ANIMALS; DNA PRIMERS; GENETIC VARIATION; HEK293 CELLS; HUMANS; KINETICS; MASS SPECTROMETRY; PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE; PROTEIN ISOFORMS; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 78649570818     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20100490     Document Type: Article

References (62)

1. Vance, J. E. (2008) Phosphatidylserine and phosphatidylethanolamine in mammalian cells: two metabolically related aminophospholipids. J. Lipid Res. 49, 1377-1387
2. Li, Z. and Vance, D. E. (2008) Phosphatidylcholine and choline homeostasis. J. Lipid Res. 49, 1187-1194
3. Arondel, V., Benning, C. and Somerville, C. R. (1993) Isolation and functional expression in Escherichia coli of a gene encoding phosphatidylethanolamine methyltransferase (EC 2.1.1.17) from Rhodobacter sphaeroides. J. Biol. Chem. 268, 16002-16008
4. Vance, D. E., Li, Z. and Jacobs, R. L. (2007) Hepatic phosphatidylethanolamine N-methyltransferase, unexpected roles in animal biochemistry and physiology. J. Biol. Chem. 282, 33237-33241
5. Shindou, H., Hishikawa, D., Harayama, T., Yuki, K. and Shimizu, T. (2009) Recent progress on acyl CoA: lysophospholipid acyltransferase research. J. Lipid Res. 50, S46-S51
6. Nagan, N. and Zoeller, R. A. (2001) Plasmalogens: biosynthesis and functions. Prog. Lipid Res. 40, 199-229
7. Walkey, C. J., Shields, D. J. and Vance, D. E. (1999) Identification of three novel cDNAs for human phosphatidylethanolamine N-methyltransferase and localization of the human gene on chromosome 17p11.2. Biochim. Biophys. Acta 1436, 405-412
8. Shields, D. J., Agellon, L. B. and Vance, D. E. (2001) Structure, expression profile and alternative processing of the human phosphatidylethanolamine N-methyltransferase ( PEMT) gene. Biochim. Biophys. Acta 1532, 105-114
9. Resseguie, M., Song, J., Niculescu, M. D., da Costa, K. A., Randall, T. A. and Zeisel, S. H. (2007) Phosphatidylethanolamine N-methyltransferase ( PEMT) gene expression is induced by estrogen in human and mouse primary hepatocytes. FASEB J. 21, 2622-2632
10. Shields, D. J., Lingrell, S., Agellon, L. B., Brosnan, J. T. and Vance, D. E. (2005) Localization-independent regulation of homocysteine secretion by phosphatidylethanolamine N-methyltransferase. J. Biol. Chem. 280, 27339-27344
11. DeLong, C. J., Shen, Y. J., Thomas, M. J. and Cui, Z. (1999) Molecular distinction of phosphatidylcholine synthesis between the CDP-choline pathway and phosphatidylethanolamine methylation pathway. J. Biol. Chem. 274, 29683-29688
12. Walkey, C. J., Yu, L., Agellon, L. B. and Vance, D. E. (1998) Biochemical and evolutionary significance of phospholipid methylation. J. Biol. Chem. 273, 27043-27046
13. Song, J., da Costa, K. A., Fischer, L. M., Kohlmeier, M., Kwock, L., Wang, S. and Zeisel, S. H. (2005) Polymorphism of the PEMT gene and susceptibility to nonalcoholic fatty liver disease (NAFLD). FASEB J. 19, 1266-1271
14. Dong, H., Wang, J., Li, C., Hirose, A., Nozaki, Y., Takahashi, M., Ono, M., Akisawa, N., Iwasaki, S., Saibara, T. and Onishi, S. (2007) The phosphatidylethanolamine N-methyltransferase gene V175M single nucleotide polymorphism confers the susceptibility to NASH in Japanese population. J. Hepatol. 46, 915-920
15. Li, Z., Agellon, L. B., Allen, T. M., Umeda, M., Jewell, L., Mason, A. and Vance, D. E. (2006) The ratio of phosphatidylcholine to phosphatidylethanolamine influences membrane integrity and steatohepatitis. Cell Metab. 3, 321-331
16. Zhao, Y., Su, B., Jacobs, R. L., Kennedy, B., Francis, G. A., Waddington, E., Brosnan, J. T., Vance, J. E. and Vance, D. E. (2009) Lack of phosphatidylethanolamine N-methyltransferase alters plasma VLDL phospholipids and attenuates atherosclerosis in mice. Arterioscler. Thromb. Vasc. Biol. 29, 1349-1355
17. Morita, S. Y., Kobayashi, A., Takanezawa, Y., Kioka, N., Handa, T., Arai, H., Matsuo, M. and Ueda, K. (2007) Bile salt-dependent efflux of cellular phospholipids mediated by ATP binding cassette protein B4. Hepatology 46, 188-199
18. Chen, Y. Q., Kuo, M. S., Li, S., Bui, H. H., Peake, D. A., Sanders, P. E., Thibodeaux, S. J., Chu, S., Qian, Y. W., Zhao, Y. et al. (2008) AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase. J. Biol. Chem. 283, 10048-10057
19. Jain, S., Zhang, X., Khandelwal, P. J., Saunders, A. J., Cummings, B. S. and Oelkers, P. (2009) Characterization of human lysophospholipid acyltransferase 3. J. Lipid Res. 50, 1563-1570
20. Newman, E. A., Muh, S. J., Hovhannisyan, R. H., Warzecha, C. C., Jones, R. B., McKeehan, W. L. and Carstens, R. P. (2006) Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual color fluorescence minigene assays. RNA 12, 1129-1141
21. Ridgway, N. D. and Vance, D. E. (1992) Phosphatidylethanolamine N-methyltransferase from rat liver. Methods Enzymol. 209, 366-374
22. Bligh, E. G. and Dyer, W. J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917
23. Zhou, X. and Arthur, G. (1992) Improved procedures for the determination of lipid phosphorus by malachite green. J. Lipid Res. 33, 1233-1236
24. Stone, S. J., Cui, Z. and Vance, J. E. (1998) Cloning and expression of mouse liver phosphatidylserine synthase-1 cDNA: overexpression in rat hepatoma cells inhibits the CDP-ethanolamine pathway for phosphatidylethanolamine biosynthesis. J. Biol. Chem. 273, 7293-7302
25. Leidl, K., Liebisch, G., Richter, D. and Schmitz, G. (2008) Mass spectrometric analysis of lipid species of human circulating blood cells. Biochim. Biophys. Acta 1781, 655-664
26. Taguchi, R., Houjou, T., Nakanishi, H., Yamazaki, T., Ishida, M., Imagawa, M. and Shimizu, T. (2005) Focused lipidomics by tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 823, 26-36
27. Takayama, M., Itoh, S., Nagasaki, T. and Tanimizu, I. (1977) A new enzymatic method for determination of serum choline-containing phospholipids. Clin. Chim. Acta 79, 93-98
28. Hojjati, M. R. and Jiang, X. C. (2006) Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J. Lipid Res. 47, 673-676
29. Morita, S. Y., Ueda, K. and Kitagawa, S. (2009) Enzymatic measurement of phosphatidic acid in cultured cells. J. Lipid Res. 50, 1945-1952
30. Artiss, J. D., McGowan, M. W., Strandbergh, D. R., Epstein, E. and Zak, B. (1984) Enzymic colorimetric determination of phosphatidylglycerol in amniotic fluid. Clin. Chem. 30, 534-537
31. Blaton, V., De Buyzere, M., Spincemaille, J. and Declercq, B. (1983) Enzymic assay for phosphatidylcholine and sphingomyelin in serum. Clin. Chem. 29, 806-809
32. He, X., Chen, F., McGovern, M. M. and Schuchman, E. H. (2002) A fluorescence-based, high-throughput sphingomyelin assay for the analysis of Niemann-Pick disease and other disorders of sphingomyelin metabolism. Anal. Biochem. 306, 115-123
33. Kishimoto, T., Soda, Y., Matsuyama, Y. and Mizuno, K. (2002) An enzymatic assay for lysophosphatidylcholine concentration in human serum and plasma. Clin. Biochem. 35, 411-416
34. Aoki, J., Taira, A., Takanezawa, Y., Kishi, Y., Hama, K., Kishimoto, T., Mizuno, K., Saku, K., Taguchi, R. and Arai, H. (2002) Serum lysophosphatidic acid is produced through diverse phospholipase pathways. J. Biol. Chem. 277, 48737-48744
35. Ota, H., Tamezane, H., Sasano, Y., Hokazono, E., Yasuda, Y., Sakasegawa, S., Imamura, S., Tamura, T. and Osawa, S. (2008) Enzymatic characterization of an amine oxidase from Arthrobacter sp. used to measure phosphatidylethanolamine. Biosci. Biotechnol. Biochem. 72, 2732-2738
36. Zhou, M., Diwu, Z., Panchuk-Voloshina, N. and Haugland, R. P. (1997) A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal. Biochem. 253, 162-168
37. Ohta-Fukuyama, M., Miyake, Y., Emi, S. and Yamano, T. (1980) Identification and properties of the prosthetic group of choline oxidase from Alcaligenes sp. J. Biochem. 88, 197-203
38. Weerheim, A. M., Kolb, A. M., Sturk, A. and Nieuwland, R. (2002) Phospholipid composition of cell-derived microparticles determined by one-dimensional high-performance thin-layer chromatography. Anal. Biochem. 302, 191-198
39. Wakelam, M. J., Pettitt, T. R. and Postle, A. D. (2007) Lipidomic analysis of signaling pathways. Methods Enzymol. 432, 233-246
40. Shields, D. J., Lehner, R., Agellon, L. B. and Vance, D. E. (2003) Membrane topography of human phosphatidylethanolamine N-methyltransferase. J. Biol. Chem. 278, 2956-2962
41. Shields, D. J., Altarejos, J. Y., Wang, X., Agellon, L. B. and Vance, D. E. (2003) Molecular dissection of the S-adenosylmethionine-binding site of phosphatidylethanolamine N-methyltransferase. J. Biol. Chem. 278, 35826-35836
42. Voelker, D. R. (1991) Organelle biogenesis and intracellular lipid transport in eukaryotes. Microbiol. Rev. 55, 543-560
43. Lee, M. W., Bakovic, M. and Vance, D. E. (1996) Overexpression of phosphatidylethanolamine N-methyltransferase 2 in CHO-K1 cells does not attenuate the activity of the CDP-choline pathway for phosphatidylcholine biosynthesis. Biochem. J. 320, 905-910
44. Ridgway, N. D. and Vance, D. E. (1988) Specificity of rat hepatic phosphatidylethanolamine N-methyltransferase for molecular species of diacyl phosphatidylethanolamine. J. Biol. Chem. 263, 16856-16863
45. Sato, H., Taketomi, Y., Isogai, Y., Miki, Y., Yamamoto, K., Masuda, S., Hosono, T., Arata, S., Ishikawa, Y., Ishii, T. et al. (2010) Group III secreted phospholipase A2 regulates epididymal sperm maturation and fertility in mice. J. Clin. Invest. 120, 1400-1414
46. Bleijerveld, O. B., Brouwers, J. F., Vaandrager, A. B., Helms, J. B. and Houweling, M. (2007) The CDP-ethanolamine pathway and phosphatidylserine decarboxylation hatidylethanolamine molecular species. J. Biol. Chem. 282, 28362-28372
47. Arthur, G. and Page, L. (1991) Synthesis of phosphatidylethanolamine and ethanolamine plasmalogen by the CDP-ethanolamine and decarboxylase pathways in rat heart, kidney and liver. Biochem. J. 273, 121-125
48. Xu, Z. L., Byers, D. M., Palmer, F. B., Spence, M. W. and Cook, H. W. (1991) Serine utilization as a precursor of phosphatidylserine and alkenyl-(plasmenyl)-, alkyl-, and acylethanolamine phosphoglycerides in cultured glioma cells. J. Biol. Chem. 266, 2143-2150
49. Lee, T. C., Qian, C. G. and Snyder, F. (1991) Biosynthesis of choline plasmalogens in neonatal rat myocytes. Arch. Biochem. Biophys. 286, 498-503
50. Mozzi, R., Siepi, D., Andreoli, V. and Porcellati, G. (1981) The synthesis of choline plasmalogen by the methylation pathway in rat brain. FEBS Lett. 131, 115-118 (Pubitemid 12225968)
51. Mogelson, S. and Sobel, B. E. (1981) Ethanolamine plasmalogen methylation by rabbit myocardial membranes. Biochim. Biophys. Acta 666, 205-211
52. Vance, J. E. (1990) Lipoproteins secreted by cultured rat hepatocytes contain the antioxidant 1-alk-1-enyl-2-acylglycerophosphoethanolamine. Biochim. Biophys. Acta 1045, 128-134
53. Jurgens, G., Fell, A., Ledinski, G., Chen, Q. and Paltauf, F. (1995) Delay of copper-catalyzed oxidation of low density lipoprotein by in vitro enrichment with choline or ethanolamine plasmalogens. Chem. Phys. Lipids 77, 25-31
54. Maeba, R., Maeda, T., Kinoshita, M., Takao, K., Takenaka, H., Kusano, J., Yoshimura, N., Takeoka, Y., Yasuda, D., Okazaki, T. and Teramoto, T. (2007) Plasmalogens in human serum positively correlate with high-density lipoprotein and decrease with aging. J. Atheroscler. Thromb. 14, 12-18
55. Cui, Z., Houweling, M. and Vance, D. E. (1994) Suppression of rat hepatoma cell growth by expression of phosphatidylethanolamine N-methyltransferase-2. J. Biol. Chem. 269, 24531-24533
56. Tessitore, L., Sesca, E., Bosco, M. and Vance, D. E. (1999) Expression of phosphatidylethanolamine N-methyltransferase in Yoshida ascites hepatoma cells and the livers of host rats. Carcinogenesis 20, 561-567
57. Houweling, M., Cui, Z. and Vance, D. E. (1995) Expression of phosphatidylethanolamine N-methyltransferase-2 cannot compensate for an impaired CDP-choline pathway in mutant Chinese hamster ovary cells. J. Biol. Chem. 270, 16277-16282
58. Emoto, K., Kobayashi, T., Yamaji, A., Aizawa, H., Yahara, I., Inoue, K. and Umeda, M. (1996) Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis. Proc. Natl. Acad. Sci. U.S.A. 93, 12867-12872
59. Emoto, K. and Umeda, M. (2000) An essential role for a membrane lipid in cytokinesis: regulation of contractile ring disassembly by redistribution of phosphatidylethanolamine. J. Cell Biol. 149, 1215-1224
60. Kyte, J. and Doolittle, R. F. (1982) A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105-132
61. Hirokawa, T., Boon-Chieng, S. and Mitaku, S. (1998) SOSUI: classification and secondary structure prediction system for membrane proteins. Bioinformatics 14, 378-379
62. Farooqui, J. Z., Lee, H. W., Kim, S. and Paik, W. K. (1983) Studies on compartmentation of S-adenosyl-L-methionine in Saccharomyces cerevisiae and isolated rat hepatocytes. Biochim. Biophys. Acta 757, 342-351