A fluorescence-based, high-throughput sphingomyelin assay for the analysis of Niemann-Pick disease and other disorders of sphingomyelin metabolism

Analytical Biochemistry

Volumn 306, Issue 1, 2002, Pages 115-123

  He, Xingxuan ^a   Chen, Fei ^a   McGovern, Margaret M ^a   Schuchman, Edward H ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Amplex Red; Dihydroxyphenoxazine; Fluorescence detection; Niemann Pick disease; Sphingomyelin

Indexed keywords

CERAMIDES; CYTOLOGY; ENZYME ACTIVITY; FLUORESCENCE; HISTOLOGY; HYDROGEN PEROXIDE; HYDROLYSIS; MAMMALS; OXIDATION; PEROXIDES; PHOSPHATASES; PRODUCT DEVELOPMENT; RADIOACTIVITY; TISSUE; TISSUE ENGINEERING;

96-WELL MICROTITER PLATE; ACID SPHINGOMYELINASE; AMPLEX RED; DIACYLGLYCEROL KINASE; DIHYDROXYPHENOXAZINE; FLUORESCENCE DETECTION; FLUORESCENCE DETECTOR; SPHINGOMYELIN;

PHOSPHOLIPIDS;

ALKALINE PHOSPHATASE; CERAMIDE; DIACYLGLYCEROL KINASE; HYDROGEN PEROXIDE; PHENOXAZINE DERIVATIVE; PHOSPHORYLCHOLINE; SPHINGOMYELIN; SPHINGOMYELIN PHOSPHODIESTERASE; AMPLEX RED REAGENT; OXAZINE DERIVATIVE; RESORUFIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOACCUMULATION; COLORIMETRY; CONTROLLED STUDY; ENZYME DEFICIENCY; FLUORESCENCE ANALYSIS; HUMAN; HUMAN TISSUE; HYDROLYSIS; LIPIDOSIS; MOUSE; NIEMANN PICK DISEASE; NONHUMAN; PLASMA; PRIORITY JOURNAL; REACTION TIME; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TISSUE; URINE; ANIMAL; BLOOD; BLOOD ANALYSIS; BRAIN LEVEL; CHEMISTRY; FLUORESCENCE; KINETICS; LIVER; LUNG; METABOLISM; OXIDATION REDUCTION REACTION; SPLEEN;

BACTERIA (MICROORGANISMS);

ANIMALS; BLOOD CHEMICAL ANALYSIS; BRAIN CHEMISTRY; FLUORESCENCE; HUMANS; KINETICS; LIVER; LUNG; MICE; NIEMANN-PICK DISEASES; OXAZINES; OXIDATION-REDUCTION; SENSITIVITY AND SPECIFICITY; SPHINGOMYELIN PHOSPHODIESTERASE; SPHINGOMYELINS; SPLEEN;

EID: 0036629206     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5686     Document Type: Article

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