Expression of chondrogenic genes by undifferentiated vs. differentiated human mesenchymal stem cells using array technology

Bio-Medical Materials and Engineering

Volumn 20, Issue 3-4, 2010, Pages 175-181

  Henrionnet, Christel ^a   Roeder, Emilie ^a   Gillet, Romain ^a   Galois, Laurent ^a,b   Bensoussan, Danièle ^a,c   Mainard, Didier ^a,b   Netter, Patrick ^a   Gillet, Pierre ^a   Pinzano, Astrid ^a  

^a Biopôle de l Université de Lorraine   (France)

^b UNIVERSITY HOSPITAL OF NANCY   (France)

^c Unité de Thérapie Cellulaire et Tissulaire   (France)

Author keywords

collagen sponge; gene expression; growth factors; human mesenchymal stem cells; RT PCR array

Indexed keywords

ALKALINE PHOSPHATASE; BASAL MEDIUM; CHONDROGENIC; COLLAGEN SPONGE; DIFFERENTIAL EXPRESSIONS; GENE EXPRESSION PROFILES; GROWTH FACTORS; HUMAN MESENCHYMAL STEM CELLS; IN-VITRO; OSTEOGENIC DIFFERENTIATION; PCR ARRAYS; REAL TIME RT-PCR; SEQUENTIAL COMBINATION;

CELL CULTURE; COLLAGEN; PEPTIDES; PHOSPHATASES; PLANTS (BOTANY); STEM CELLS;

GENE EXPRESSION;

SIGNAL PEPTIDE;

ARTICLE; BIOTECHNOLOGY; CARTILAGE CELL; CELL CULTURE; CELL DIFFERENTIATION; CHONDROGENESIS; CYTOLOGY; GENE EXPRESSION PROFILING; HUMAN; MESENCHYMAL STEM CELL; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN MICROARRAY;

BIOTECHNOLOGY; CELL DIFFERENTIATION; CELLS, CULTURED; CHONDROCYTES; CHONDROGENESIS; GENE EXPRESSION PROFILING; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MESENCHYMAL STEM CELLS; PROTEIN ARRAY ANALYSIS;

EID: 78649502106     PISSN: 09592989     EISSN: None     Source Type: Journal    
DOI: 10.3233/BME-2010-0629     Document Type: Conference Paper

References (17)

1. E.B. Hunziker, Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects, Osteoarthr. Cartil. 10 (2002), 432-463.
2. M.F. Pittenger, A.M. Mackay, S.C. Beck, R.K. Jaiswal, R. Douglas, J.D. Mosca, M.A. Moorman, D.W. Simonetti, S. Craig and D.R. Marshak, Multilineage potential of adult human mesenchymal stem cells, Science 284 (1999), 143-147.
3. P. Behrens, T. Bitter, B. Kurz and M. Russlies, Matrix-associated autologous chondrocyte transplantation/implantation (MACT/MACI)-5-year follow-up, Knee 13 (2006), 194-202.
4. H. Chajra, C.F. Rousseau, D. Cortial, M.C. Ronziere, D. Herbage, F. Mallein-Gerin and A.M. Freyria, Collagen-based biomaterials and cartilage engineering. Application to osteochondral defects, Biomed. Mater. Eng. 18 (2008), S33-S45.
5. R. Kuroda, K. Ishida, T. Matsumoto, T. Akisue, H. Fujioka, K. Mizuno, H. Ohgushi, S. Wakitani and M. Kurosaka, Treatment of a full-thickness articular cartilage defect in the femoral condyle of an athlete with autologous bone-marrow stromal cells, Osteoarthr. Cartil. 15 (2007), 226-231.
6. C. Vinatier, D. Mrugala, C. Jorgensen, J. Guicheux and D. Noel, Cartilage engineering: a crucial combination of cells, biomaterials and biofactors, Trends Biotechnol. 27 (2009), 307-314.
7. K.J. Livak and T.D. Schmittgen, Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method, Methods 25 (2001), 402-408.
8. D.C. Wescott, M.N. Pinkerton, B.J. Gaffey, K.T. Beggs, T.J. Milne and M.C. Meikle, Osteogenic gene expression by human periodontal ligament cells under cyclic tension, J. Dent. Res. 86 (2007), 1212-1216.
9. M.B. Goldring, Update on the biology of the chondrocyte and new approaches to treating cartilage diseases, Best Pract. Res. Clin. Rheumatol. 20 (2006), 1003-1025.
10. U. Valcourt, J. Gouttenoire, E. Aubert-Foucher, D. Herbage and F. Mallein-Gerin, Alternative splicing of type II procol-lagen pre-mRNA in chondrocytes is oppositely regulated by BMP-2 and TGF-beta1, FEBS Lett. 545 (2003), 115-119.
11. F.H. Chen and R.S. Tuan, Mesenchymal stem cells in arthritic diseases, Arthritis Res. Ther. 10 (2008), 223.
12. I. Sekiya, B.L. Larson, J.T. Vuoristo, R.L. Reger and D.J. Prockop, Comparison of effect of BMP-2,-4, and-6 on in vitro cartilage formation of human adult stem cells from bone marrow stroma, Cell Tissue Res. 320 (2005), 269-276.
13. N. Indrawattana, G. Chen, M. Tadokoro, L.H. Shann, H. Ohgushi, T. Tateishi, J. Tanaka and A. Bunyaratvej, Growth factor combination for chondrogenic induction from human mesenchymal stem cell, Biochem. Biophys. Res. Commun. 320 (2004), 914-919.
14. S.M. Richardson, J.A. Hoyland, R. Mobasheri, C. Csaki, M. Shakibaei and A. Mobasheri, Mesenchymal stem cells in regenerative medicine: opportunities and challenges for articular cartilage and intervertebral disc tissue engineering, J. Cell. Physiol. 222 (2010), 23-32.
15. M.B. Goldring and K.B. Marcu, Cartilage homeostasis in health and rheumatic diseases, Arthritis Res. Ther. 11 (2009), 224.
16. V. Lefebvre, R.R. Behringer and B. de Crombrugghe, L-Sox5, Sox6 and Sox9 control essential steps of the chondrocyte differentiation pathway, Osteoarthr. Cartil. 9 (Suppl. A) (2001), S69-S75.
17. C. Csaki, P.R. Schneider and M. Shakibaei, Mesenchymal stem cells as a potential pool for cartilage tissue engineering, Ann. Anat. 190 (2008), 395-412.