Cervimycin C resistance in Bacillus subtilis is due to a promoter up-mutation and increased mRNA stability of the constitutive ABC-transporter gene bmrA

FEMS Microbiology Letters

Volumn 313, Issue 2, 2010, Pages 155-163

  Krügel, Hans ^a   Licht, Andreas ^b   Biedermann, Gesine ^a   Petzold, Andreas ^c   Lassak, Jürgen ^a   Hupfer, Yvonne ^a   Schlott, Bernhard ^c   Hertweck, Christian ^a   Platzer, Matthias ^c   Brantl, Sabine ^b   Saluz, Hans Peter ^a  

^a HANS KNÖLL INSTITUTE   (Germany)

^b FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^c FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

ABC exporter; Bacillus subtilis; Cervimycin; Promoter up mutation; YvcC BmrA

Indexed keywords

ABC TRANSPORTER; ANTIBIOTIC AGENT; CERVIMYCIN C; DNA FRAGMENT; DOXORUBICIN; HEPARIN; LINCOMYCIN; MEMBRANE PROTEIN; MESSENGER RNA; PUROMYCIN; RNA POLYMERASE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; BACILLUS SUBTILIS; BACTERIAL STRAIN; CONTROLLED STUDY; DRUG HALF LIFE; GEL MOBILITY SHIFT ASSAY; GENE MUTATION; GENE SEQUENCE; IN VITRO GENE TRANSFER; LETTER; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ISOLATION; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; RNA STABILITY;

ANTHRACYCLINES; ANTI-BACTERIAL AGENTS; ARTIFICIAL GENE FUSION; ATP-BINDING CASSETTE TRANSPORTERS; BACILLUS SUBTILIS; DRUG RESISTANCE, BACTERIAL; ELECTROPHORETIC MOBILITY SHIFT ASSAY; GENE EXPRESSION; GENES, REPORTER; MUTATION; PROMOTER REGIONS, GENETIC; RNA STABILITY;

BACILLUS SUBTILIS;

EID: 78649496843     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02143.x     Document Type: Letter

References (28)

1. Andries K, Verhasselt P, Guillemont J et al. (2005) A diarylquinoline drug active on the ATP synthase of Mycobacterium tuberculosis. Science 307: 223-227.
2. Barbe V, Cruveiller S, Kunst F et al. (2009) From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later. Microbiology 155: 1758-1775.
3. Cáceres NE, Harris NB, Wellehan JF, Feng Z, Kapur V & Barletta RG (1997) Overexpression of the d-alanine racemase gene confers resistance to d-cycloserine in Mycobacterium smegmatis. J Bacteriol 179: 5046-5055.
4. Chami M, Steinfels E, Orelle C, Jault JM, Di Pietro A, Rigaud JL & Marco S (2002) Three-dimensional structure by cryo-electron microscopy of YvcC, a homodimeric ATP-binding cassette transporter from Bacillus subtilis. J Mol Biol 315: 1075-1085.
5. Depardieu F, Podglajen I, Leclercq R, Collatz E & Courvalin P (2007) Modes and modulations of antibiotic resistance gene expression. Clin Microbiol Rev 20: 79-114.
6. Hambraeus G, Persson M & Rutberg B (2000) The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis. Microbiology 146: 3051-3059.
7. Hansen MJ, Chen LH, Fejzo ML & Belasco JG (1994) The ompA 5' untranslated region impedes a major pathway for mRNA degradation in Escherichia coli. Mol Microbiol 12: 707-716.
8. Heidrich N, Chinali A, Gerth U & Brantl S (2006) The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism. Mol Microbiol 62: 520-536.
9. Herold K, Gollmick FA, Groth I, Roth M, Menzel KD, Möllmann U, Gräfe U & Hertweck C (2005) Cervimycin A-D: a polyketide glycoside complex from a cave bacterium can defeat vancomycin resistance. Chemistry 11: 5523-5530.
10. Kerr ID, Reynolds ED & Cove JH (2005) ABC proteins and antibiotic drug resistance: is it all about transport? Biochem Soc Trans 33: 1000-1002.
11. Leaver M, Dominguez-Cuevas P, Coxhead JM, Daniel RA & Errington J (2009) Life without a wall or division machine in Bacillus subtilis. Nature 457: 849-853.
12. Licht A, Preis S & Brantl S (2005) Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in B. subtilis. Mol Microbiol 58: 189-206.
13. Licht A, Golbik R & Brantl S (2008) Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis. J Mol Biol 380: 17-30.
14. Makarov V, Manina G, Mikusova K et al. (2009) Benzothiazinones kill Mycobacterium tuberculosis by blocking arabinan synthesis. Science 324: 801-804.
15. Mima T, Joshi S, Gomez-Esmeralda M & Schweizer HP (2007) Identification and characterization of TriABC-OmpH, a triclosan efflux pump of Pseudomonas aeuginosa requiring two membrane fusion proteins. J Bacteriol 189: 7600-7609.
16. Mwangi MM, Wu SW, Zhou Y et al. (2007) Tracking the in vivo evolution of multidrug resistance in Staphylococcus aureus by whole-genome sequencing. P Natl Acad Sci USA 104: 9451-9456.
17. Ohki R & Tateno K (2004) Increased stability of bmr3 mRNA results in a multidrug-resistance phenotype in Bacillus subtilis. J Bacteriol 186: 7450-7455.
18. Orelle C, Dalmas O, Gros P, Di Pietro A & Jault JM (2003) The conserved glutamate residue adjacent to the Walker-B motif is the catalytic base for ATP hydrolysis in the ATP-binding cassette transporter BmrA. J Biol Chem 278: 47002-47008.
19. Orelle C, Gubellini F, Durand A, Marco S, Lévy D, Gros P, Di Pietro A & Jault JM (2008) Conformational change induced by ATP binding in the multidrug ATP-binding cassette transporter BmrA. Biochemistry 47: 2404-2412.
20. Pfaffl MW (2002) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: 2002-2007.
21. Piddock LJV (2006) Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria. Clin Microbiol Rev 19: 382-402.
22. Ravaud S, Do Cao MA, Jidenko M, Ebel C, Le Maire M, Jault JM, Di Pietro A, Haser R & Aghajari N (2006) The ABC transporter BmrA from Bacillus subtilis is a functional dimer when in a detergent-solubilised state. Biochem J 395: 345-353.
23. Srivatsan A, Han Y, Peng J, Tehranchi AK, Gibbs R, Wang JD & Chen R (2008) High-precision whole-genome sequencing of laboratory strains facilitates genetic studies. PLOS Genet R 457: 849-853.
24. Steinfels E, Orelle C, Dalmas O, Penin F, Miroux B, Di Pietro A & Jault JM (2002) Highly efficient over-production in E. coli of YvcC, a multidrug-like ATP-binding cassette transporter from Bacillus subtilis. Biochem Biophys Acta 1565: 1-5.
25. Steinfels E, Orelle C, Fantino JR, Dalmas O, Rigaud JL, Denizot F, Di Pietro A & Jault JM (2004) Characterization of YvcC (BmrA), a multidrug ABC transporter constitutively expressed in Bacillus subtilis. Biochemistry 43: 7491-7502.
26. Stirrett KL, Ferreras JA, Rossi SM, Moy RL, Fonseca FV & Quadri LE (2008) A multicopy suppressor screening approach as a means to identify antibiotic resistance determinant candidates in Yersinia pestis. BMC Microbiol 8: 122.
27. Stülke J, Martin-Verstraete I, Zagorec M, Rose M, Klier A & Rapoport G (1997) Induction of the Bacillus subtilisptsGHI operon by glucose is controlled by a novel antiterminator GlcT. Mol Microbiol 25: 65-78.
28. Wright GD (2000) Resisting resistance: new chemical strategies for battling superbugs. Chem Biol 7: R127-R132.