Spectrum of haemoglobinopathies diagnosed by cation exchange-HPLC & modulating effects of nutritional deficiency anaemias from north India

Indian Journal of Medical Research

Volumn 132, Issue 11, 2010, Pages 513-519

  Rao, Seema ^a   Kar, Rakhee ^a   Gupta, Sanjeev Kumar ^a   Chopra, Anita ^a   Saxena, Renu ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Diagnosis; Haemoglobinopathies; HPLC; Nutritional deficiency anaemia; Thalassaemia

Indexed keywords

HEMOGLOBIN; HEMOGLOBIN A; HEMOGLOBIN A2; HEMOGLOBIN D; HEMOGLOBIN E; HEMOGLOBIN H; HEMOGLOBIN J; HEMOGLOBIN S; HEMOGLOBIN VARIANT;

ADOLESCENT; ADULT; AGED; ANALYTIC METHOD; ANALYTICAL EQUIPMENT; ANEMIA; ARTICLE; BETA THALASSEMIA; BLOOD SAMPLING; CATION EXCHANGE; CHILD; ELECTROPHORESIS; ERYTHROCYTE COUNT; ETHNIC GROUP; FEMALE; HEMATOLOGICAL PARAMETERS; HEMOGLOBIN DETERMINATION; HEMOGLOBINOPATHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; INDIA; INFANT; IRON DEFICIENCY ANEMIA; MAJOR CLINICAL STUDY; MALE; MEAN CORPUSCULAR HEMOGLOBIN; MEAN CORPUSCULAR VOLUME; MEGALOBLASTIC ANEMIA; NUTRITIONAL DEFICIENCY; CLASSIFICATION; EVALUATION; ION EXCHANGE CHROMATOGRAPHY; MALNUTRITION; METHODOLOGY; PROSPECTIVE STUDY;

ANEMIA; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; FEMALE; HEMOGLOBINOPATHIES; HEMOGLOBINS; HUMANS; INDIA; MALE; MALNUTRITION; PROSPECTIVE STUDIES;

EID: 78649465586     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Working Party of the General Hematology Task Force of the British Committee for Standards in Hematology. Guideline: the laboratory diagnosis of hemoglobinopathies. Br J Hematol 1998; 101: 783-92.
2. Bain BJ. Hemoglobinopathy diagnosis, 1st ed. Oxford: Blackwell Science; 2001.
3. Tyagi S, Saxena R, Choudhry VP. HPLC - how necessary is it for haemoglobinopathy diagnosis in India? Indian J Pathol Microbiol 2003; 46: 390-3.
4. Rangan A, Handoo A, Sinha S, Saxena R, Verma IC, Kumar S, et al. Utility of family studies in diagnosing abnormal hemoglobins/thalassemic states. Indian J Pediatr 2009; 76: 615-21.
5. Hardison RC, Chui DHK, Giardine B, Riemer C, Patrinos GP, Anagnou N, et al. HbVar: a relational database of human hemoglobin variants and thalassemia mutations at the globin gene server. Hum Mut 2002; 19: 225-33.
6. Joutovsky A, Hadzi-Nesic J, Nardi MA. HPLC retention time as a diagnostic tool for hemoglobin variants and hemoglobinopathies: A study of 60000 samples in a clinical diagnostic laboratory. Clin Chem 2004; 50: 1736-47.
7. Colah RB, Wadia M, Surve R, Nadkarni A, Phanasgaonkar S, Gorakshakar A, et al. Hb D Agri [ b9 (A6) Ser ® Tyr, b 121 (GH - 4) Glu ® Gln]: A new Indian hemoglobin variant with two amino acid substitutions in the same beta chain. Hemoglobin 2001; 25: 317-21.
8. Ou CN, Rognerud CL. Diagnosis of hemoglobinopathies: electrophoresis vs. HPLC. Clin Chim Acta 2001; 313: 187-94.
9. Riou J, Godart C, Didier H, Mathis M, Bimet C, Bardakdjian- Michau J, et al. Cation-exchange HPLC evaluated for presumptive identification of hemoglobin variants. Clin Chem 1997; 43: 34-9.
10. Eastman JW, Wong R, Liao CL, Morales DR. Automated HPLC screening of newborns for sickle cell anemia and other hemoglobinopathies. Clin Chem 1996; 42: 704-10.
11. Eastman JW, Lorey F, Arnopp J, Currier RJ, Sherwin J, Cunningham G. Distribution of hemoglobin F, A, S, C, E and D quantities in 4 million newborn screening specimens. Clin Chem 1999; 45: 683-5.
12. Mario N, Baudin B, Aussel C, Giboudeau J. Capillary isoelectric focusing and high-performance cation-exchange chromatography compared for qualitative and quantitative analysis of hemoglobin variants. Clin Chem 1997; 43: 2137-42.
13. Fucharoen S, Winichagoon P, Wisedpanichkij R, Sae-Ngow B, Sriphanich R, Oncoung W, et al. Prenatal and postnatal diagnoses of thalassemias and hemoglobinopathies by HPLC. Clin Chem 1998; 44: 740-8.
14. Mario N, Baudin B, Aussel C, Giboudeau J. Capillary isoelectric focusing and high-performance cation-exchange chromatography compared for qualitative and quantitative analysis of hemoglobin variants. Clin Chem 1997; 43: 2137-42.
15. Colah RB, Surve R, Sawant P, D'Souza E, Italia K, Phanasgaonkar S, et al. HPLC studies in hemoglobinopathies. Indian J Pediatr 2007; 74: 657-62.
16. Pignatti CB, Galanello R. Thalassemias and related disorders: quantitative disorders of hemoglobin synthesis. In: Greer JP, Foerster J, Lukens JN, Rodgers GM, Paraskevas F, Glader B, editors. Wintrobes clinical hematology, 11th ed. Philedelphia: Lippincott Williams and Wilkins; 2004. p. 1319-65.
17. Cotton F, Gulbis B, Hansen V, Vertongen F. Interference of hemoglobin D in hemoglobin A2 measurement by cation-exchange HPLC. Clin Chem 1999; 45: 1317-8.
18. Dash S. HbA2 in subjects with HbD. Clin Chem 1998; 44: 2381-2.
19. Suh DD, Kraus JS, Bures K. Influence of hemoglobin S adducts on hemoglobin A2 quantification by HPLC. Clin Chem 1996; 42: 1113-4.
20. Nagel RL, Steinberg MH. Hemoglobin SC disease and HbC disorders. In: Steinberg MH, Forget BG, Higgs DR, Nagel RL, editors. Disorders of hemoglobin: genetics, pathophysiology, and clinical management. New York: Cambridge University Press; 2001. p. 756-85.
21. Steinberg MH. Compound heterozygous and other sickle hemoglobinopathies. In: Steinberg MH, Forget BG, Higgs DR, Nagel RL, editors. Disorders of hemoglobin: genetics, pathophysiology, and clinical management. New York: Cambridge University Press; 2001. p. 786-810.
22. Alperin JB, Dow PA, Petteway MB. Hemoglobin A2 levels in health and various hematologic disorders. Am J Clin Pathol 1977; 67: 219-26.
23. Madan N, Sikka M, Sharma S, Rusia U. Phenotypic expression of hemoglobin A2 in beta-thalassemia trait with iron deficiency. Ann Hematol 1998; 77: 93-6.
24. Giambona A, Passarello C, Vinciguerra M, Li Muli R, Teresi P, Anzà M, et al. Significance of borderline hemoglobin A2 values in an Italian population with a high prevalence of b-thalassemia. Haematologica 2008; 93:1380-4.