Construction of expression vectors for protein production in Gluconobacter oxydans

Journal of Biotechnology

Volumn 150, Issue 4, 2010, Pages 460-465

  Kallnik, Verena ^a   Meyer, Maria ^a   Deppenmeier, Uwe ^a   Schweiger, Paul ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

Broad host range; Host vector system; Industrial biotechnology; Overexpression; Plasmid

Indexed keywords

BROAD-HOST-RANGE; HOST-VECTOR SYSTEM; INDUSTRIAL BIOTECHNOLOGY; OVER-EXPRESSION; PLASMID;

ACIDS; BIOTECHNOLOGY; ENCODING (SYMBOLS); GENE ENCODING; INDUSTRY; MICROBIOLOGY; PROTEINS; SUGAR (SUCROSE); VECTORS;

GENE EXPRESSION;

BACTERIAL PROTEIN; BACTERIAL VECTOR; PLASMID VECTOR; RIBOSOMAL PROTEIN L13; RIBOSOMAL PROTEIN L35; RIBOSOME PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BIOTECHNOLOGY; CONTROLLED STUDY; DNA FLANKING REGION; EXPRESSION VECTOR; GENE CONSTRUCT; GENE EXPRESSION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC CODE; GENETIC IDENTIFICATION; GENOME; GLUCONOBACTER OXYDANS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SECRETION; REPORTER GENE;

BIOTECHNOLOGY; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC VECTORS; GLUCONOBACTER OXYDANS; GLUCURONIDASE; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; RIBOSOMAL PROTEINS;

GLUCONOBACTER; GLUCONOBACTER OXYDANS; GLUCONOBACTER OXYDANS 621H;

EID: 78649449266     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.10.069     Document Type: Article

References (37)

1. Adachi O., Moonmangmee D., Toyama H., Yamada M., Shinagawa E., Matsushita K. New developments in oxidative fermentation. Appl. Microbiol. Biotechnol. 2003, 60:643-653.
2. Antoine R., Locht C. Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from Gram-positive organisms. Mol. Microbiol. 1992, 6:1785-1799.
3. Beppu T. Genetic organization of Acetobacter for acetic acid fermentation. Antonie van Leeuwenhoek 1993, 64:121-135.
4. Blum H., Beier H., Gross H.J. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 1987, 8:93-99.
5. Buch A.D., Archana G., Kumar N.G. Broad-host-range plasmid-mediated metabolic perturbations in Pseudomonas fluorescens 13525. Appl. Microbiol. Biotechnol. 2010, 88:209-218.
6. Cheng H., Jiang N., Shen A., Feng Y. Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli. FEMS Microbiol. Lett. 2005, 252:35-42.
7. Condon C., FitzGerald R.J., O'Gara F. Conjugation and heterologous gene expression in Gluconobacter oxydans ssp. suboxydans. FEMS Microbiol. Lett. 1991, 80:173-178.
8. Creavan S., FitzGerald R.J., O'Gara F. Transformation of Gluconobacter suboxydans by electroporation. Can. J. Microbiol. 1994, 40:491-494.
9. De Ley J., Gillis M., Swings J. The genus Gluconobacter. Bergey's Manual of Systematic Bacteriology 1984, vol. 1:267-278. Williams and Wilkins, Baltimore.
10. Dumon-Seignovert L., Cariot G., Vuillard L. The toxicity of recombinant proteins in Escherichia coli: a comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3). Protein Expr. Purif. 2004, 37:203-206.
11. Fukaya M., Okumura H., Masai H., Uozumi T., Beppu T. Development of a host-vector system for Gluconobacter suboxydans. Agric. Biol. Chem. 1985, 49:2407-2411.
12. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 1983, 166:557-580.
13. Kovach M.E., Elzer P.H., Hill D.S., Robertson G.T., Farris M.A., Roop R.M., Peterson K.M. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995, 166:175-176.
14. Kovach M.E., Phillips R.W., Elzer P.H., Roop I.I., Peterson R.M.K.M. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994, 16:801-802.
15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
16. Merfort M., Herrmann U., Ha S.W., Elfari M., Bringer-Meyer S., Görisch H., Sahm H. Modification of the membrane-bound glucose oxidation system in Gluconobacter oxydans significantly increases gluconate and 5-keto-d-gluconic acid accumulation. Biotechnol. J. 2006, 1:556-563.
17. Merfort M., Herrmann U., Bringer-Meyer S., Sahm H. High-yield 5-keto-d-gluconic acid formation is mediated by soluble and membrane-bound gluconate-5-dehydrogenases of Gluconobacter oxydans. Appl. Microbiol. Biotechnol. 2006, 73:443-451.
18. Miller J.H. Unit VII: Assays of lac operon enzymes: β-galactosidase, permease, repressor, transacetylase, a complementation. Experiments in Molecular Genetics 1972, 352-355. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. J.H. Miller (Ed.).
19. Miller W.G., Leveau J.H., Lindow S.E. Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol. Plant Microbe Interact. 2000, 13:1243-1250.
20. Miroux B., Walker J.E. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 1996, 260:289-298.
21. Mostafa H.E., Heller K.J., Geis A. Cloning of Escherichia coli lacZ and lacY genes and their expression in Gluconobacter oxydans and Acetobacter liquefaciens. Appl. Environ. Microbiol. 2002, 68:2619-2623.
22. Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U. Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans. Nat. Biotechnol. 2005, 23:195-200.
23. Okumura H., Uozumi T., Beppu T. Construction of a plasmid vector and genetic transformation system for Acetobacter aceti. Agric. Biol. Chem. 1985, 49:1011-1017.
24. Ruijter J.M., Ramakers C., Hoogaars M.H., Karlen Y., Bakker O., van den Hoff M.J.B., Moorman A.F.M. Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Res. 2009, 37:e45. 10.1093/nar/gkp045.
25. Saichana I., Ano Y., Adachi O., Matsushita K., Toyama H. Preparation of enzymes required for enzymatic quantification of 5-keto-d-gluconate and 2-keto-d-gluconate. Biosci. Biotechnol. Biochem. 2007, 71:2478-2486.
26. Saïda F. Overview on the expression of toxic gene products in Escherichia coli. Curr. Protoc. Protein Sci. 2007, 50. 5.19.1-5.19.13.
27. Saito Y., Ishii Y., Hayashi H., Imao Y., Akashi T., Yoshikawa K., Noguchi Y., Soeda S., Yoshida M., Niwa M., Hosoda J., Shimomura K. Cloning of genes coding for l-sorbose and l-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-l-gulonate, a precursor of l-ascorbic acid, in a recombinant G. oxydans strain. Appl. Environ. Microbiol. 1997, 63:454-460.
28. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 2nd ed.
29. Schedel M. Regioselective oxidation of aminosorbitol with Gluconobacter oxydans, key reaction in the industrial 1-deoxynojirimycin synthesis. Biotechnology 2000, vol. 8b:295-308. Wiley-VCH, Weinheim.
30. Schleyer U., Bringer-Meyer S., Sahm H. An easy cloning and expression vector system for Gluconobacter oxydans. Int. J. Food Microbiol. 2008, 125:91-95.
31. Schweiger P., Gross H., Deppenmeier U. Characterization of two aldo-keto reductases from Gluconobacter oxydans 621H capable of regio- and stereoselective α-ketocarbonyl reduction. Appl. Microbiol. Biotechnol. 2010, 87:1415-1426.
32. Schweiger P., Gross H., Wesener S., Deppenmeier U. Vinyl ketone reduction by three distinct Gluconobacter oxydans 621H enzymes. Appl. Microbiol. Biotechnol. 2008, 80:995-1006.
33. Shinjoh M., Hoshino T. Development of a stable shuttle vector and conjugative transfer system for Gluconobacter oxydans. J. Ferment. Bioeng. 1995, 79:95-99.
34. Tonouchi N., Tsuchida T., Yoshinga F., Horinouchi S., Beppu T. A host-vector system for a cellulose-producing Acetobacter strain. Biosci. Biotechnol. Biochem. 1994, 58:1899-1901.
35. Tonouchi N., Sugiyama M., Yokozeki K. Construction of a vector plasmid for use in Gluconobacter oxydans. Biosci. Biotechnol. Biochem. 2003, 67:211-213.
36. Trček J., Raspor P., Teuber M. Molecular identification of Acetobacter isolates from submerged vinegar production, sequence analysis of plasmid pJK2-1 and application in the development of a cloning vector. Appl. Microbiol. Biotechnol. 2000, 53:289-295.
37. Zhang L., Lin J., Ma Y., Wei D., Sun M. Construction of a novel shuttle vector for use in Gluconobacter oxydans. Mol. Biotechnol. 2010, 46:227-233.