Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli

Protein Expression and Purification

Volumn 75, Issue 2, 2011, Pages 133-137

  Zheng, Chunyang ^a   Zhao, Zhanyong ^b   Li, Yan ^c   Wang, Lin ^a,b   Su, Zhiguo ^c  

^a INSTITUTE OF BIOPHYSICS   (China)

^b Biotrand Incorporation   (China)

^c INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

Apo GPO; Cofactor; FAD; Holo GPO; Specific activity

Indexed keywords

BACTERIAL PROTEIN; FLAVINE ADENINE NUCLEOTIDE; GLYCEROL 3 PHOSPHATE DEHYDROGENASE; HOLOENZYME; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT PROTEIN;

ARTICLE; CHROMATOGRAPHY; CIRCULAR DICHROISM; ENTEROCOCCUS FAECIUM; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PROTEIN CONFORMATION; SPECTROSCOPY;

BACTERIAL PROTEINS; CHROMATOGRAPHY; CIRCULAR DICHROISM; CLONING, MOLECULAR; ENTEROCOCCUS FAECIUM; ENZYME STABILITY; ESCHERICHIA COLI; FLAVIN-ADENINE DINUCLEOTIDE; GENE EXPRESSION REGULATION, BACTERIAL; GLYCEROLPHOSPHATE DEHYDROGENASE; HOLOENZYMES; ISOPROPYL THIOGALACTOSIDE; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTRUM ANALYSIS;

ESCHERICHIA COLI;

EID: 78649329059     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.08.009     Document Type: Article

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