Coimmobilization of L-α-glycerophosphate oxidase with catalase and its application for the synthesis of dihydroxyacetone phosphate

Tetrahedron

Volumn 53, Issue 43, 1997, Pages 14645-14650

  Krämer, Leonie ^a   Steckhan, Eberhard ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CATALASE; DIHYDROXYACETONE PHOSPHATE; GLYCEROL 3 PHOSPHATE DEHYDROGENASE;

ARTICLE; DECOMPOSITION; DRUG SYNTHESIS; ENZYME IMMOBILIZATION; OXIDATION; PRIORITY JOURNAL; REACTION ANALYSIS;

EID: 0342684465     PISSN: 00404020     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4020(97)01009-0     Document Type: Article

References (15)

1. Fessner, W.-D.; Sinerius, G. Angew. Chem. Int. Ed. Engl. 1994, 33, 209-212; Fessner, W.-D.; Walter, C. Topics Current Chem. 1997, 184, 97-194; Steckhan, E. Topics Current Chem. 1994, 170, 84-111; Frede, M. Ph. D. thesis, Bonn, 1993.
2. Fessner, W.-D.; Sinerius, G. Angew. Chem. Int. Ed. Engl. 1994, 33, 209-212; Fessner, W.-D.; Walter, C. Topics Current Chem. 1997, 184, 97-194; Steckhan, E. Topics Current Chem. 1994, 170, 84-111; Frede, M. Ph. D. thesis, Bonn, 1993.
3. Fessner, W.-D.; Sinerius, G. Angew. Chem. Int. Ed. Engl. 1994, 33, 209-212; Fessner, W.-D.; Walter, C. Topics Current Chem. 1997, 184, 97-194; Steckhan, E. Topics Current Chem. 1994, 170, 84-111; Frede, M. Ph. D. thesis, Bonn, 1993.
4. Fessner, W.-D.; Sinerius, G. Angew. Chem. Int. Ed. Engl. 1994, 33, 209-212; Fessner, W.-D.; Walter, C. Topics Current Chem. 1997, 184, 97-194; Steckhan, E. Topics Current Chem. 1994, 170, 84-111; Frede, M. Ph. D. thesis, Bonn, 1993.
5. After 20 h 90 % of L-α-glycerophosphate was oxidized whereas the GPO showed only 43 % of the starting activity.
6. Bossow, B.; Berke, W.; Wandrey, C. Bioeng. 1992, 8, 12-19; Huwig, A.; Daneel, H.-J.; Giffhorn, F. J. Biotechnol. 1994, 32, 309-315.
7. Bossow, B.; Berke, W.; Wandrey, C. Bioeng. 1992, 8, 12-19; Huwig, A.; Daneel, H.-J.; Giffhorn, F. J. Biotechnol. 1994, 32, 309-315.
8. Bovine serum albumin served as the standard during the spectroscopic assay with Comassie Brilliant Blue. Kresze, G.-B. in Methods of Enzymatic Analysis; Bergmeyer, H. U., Ed.; VCH: Weinheim, 1984; vol. 2, pp. 84-99.
9. Activity of GPO and catalase was usually determined by a UV-assay according to Esders, T. W.; Michrina, C. A. J. Biol. Chem. 1978, 254, 2710-2715; Claiborne, A. J. Biol. Chem. 1986, 261, 14398-14407; Aebi, H. E. in Methods of Enzymatic Analysis; Bergmeyer, H. U., Ed.; VCH: Weinheim, 1984; vol. 3, pp. 273-285.
10. Activity of GPO and catalase was usually determined by a UV-assay according to Esders, T. W.; Michrina, C. A. J. Biol. Chem. 1978, 254, 2710-2715; Claiborne, A. J. Biol. Chem. 1986, 261, 14398-14407; Aebi, H. E. in Methods of Enzymatic Analysis; Bergmeyer, H. U., Ed.; VCH: Weinheim, 1984; vol. 3, pp. 273-285.
11. Activity of GPO and catalase was usually determined by a UV-assay according to Esders, T. W.; Michrina, C. A. J. Biol. Chem. 1978, 254, 2710-2715; Claiborne, A. J. Biol. Chem. 1986, 261, 14398-14407; Aebi, H. E. in Methods of Enzymatic Analysis; Bergmeyer, H. U., Ed.; VCH: Weinheim, 1984; vol. 3, pp. 273-285.
12. ®. In this case an immobilization yield up to 84 % was achieved.
13. Lang, G. in Methods of Enzymatic Analysis; Bergmeyer, H. U., Ed.;VCH: Weinheim, 1984; vol. 6, pp. 525-531.
14. Rizzo, A. F.; Saari, L.; Lindfords, E. J. Chrom. 1986, 368, 381-386; Gilbert, J.; Startin, J. R.; Crews, C. J. Chrom. 1985, 319, 376-381.
15. Rizzo, A. F.; Saari, L.; Lindfords, E. J. Chrom. 1986, 368, 381-386; Gilbert, J.; Startin, J. R.; Crews, C. J. Chrom. 1985, 319, 376-381.