A high-resolution melting analysis for genotyping urogenital Chlamydia trachomatis

Diagnostic Microbiology and Infectious Disease

Volumn 68, Issue 4, 2010, Pages 366-374

  Li, Jian Hong ^a   Yin, Yue Ping ^a   Zheng, He Ping ^b   Zhong, Ming Ying ^a   Peng, Rui Rui ^a   Wang, Baoxi ^a   Chen, Xiang Sheng ^a  

^a INSTITUTE OF DERMATOLOGY   (China)

^b Guangdong Provincial Center for Skin Diseases and STD Control   (China)

Author keywords

Chlamydia trachomatis; Genotyping; High resolution melting analysis

Indexed keywords

BACTERIAL DNA; OUTER MEMBRANE PROTEIN A;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; CHLAMYDIA TRACHOMATIS; CHLAMYDIAL URETHRITIS; COMMENSAL; DNA DENATURATION; DNA DETERMINATION; GENOTYPE; HETERODUPLEX ANALYSIS; HIGH RESOLUTION MELTING ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SEXUALLY TRANSMITTED DISEASE;

BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL TYPING TECHNIQUES; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; GENOTYPE; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEXUALLY TRANSMITTED DISEASES, BACTERIAL; TRANSITION TEMPERATURE; UROGENITAL SYSTEM;

EID: 78649316376     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2010.07.013     Document Type: Article

References (29)

1. Brunham R.C., Kimani J., Bwayo J., Maitha G., Maclean I., Yang C., Shen C., Roman S., Nagelkerke N.J., Cheang M., Plummer F.A. The epidemiology of Chlamydia trachomatis within a sexually transmitted diseases core group. J. Infect. Dis. 1996, 173:950-956.
2. Caldwell H.D., Wood H., Crane D., Bailey R., Jones R.B., Mabey D., Maclean I., Mohammed Z., Peeling R., Roshick C., Schachter J., Solomon A.W., Stamm W.E., Suchland R.J., Taylor L., West S.K., Quinn T.C., Belland R.J., McClarty G. Polymorphisms in Chlamydia trachomatis tryptophan synthase genes differentiate between genital and ocular isolates. J. Clin. Invest. 2003, 111:1757-1769.
3. Cheng J.C., Huang C.L., Lin C.C., Chen C.C., Chang Y.C., Chang S.S., Tseng C.P. Rapid detection and identification of clinically important bacteria by high-resolution melting analysis after broad-range ribosomal RNA real-time PCR. Clin. Chem. 2006, 52:1997-2004.
4. Dayan L. Chlamydia detection and management. Aust. Fam. Physician 2000, 29:522-526.
5. Fenton K.A., Lowndes C.M. Recent trends in the epidemiology of sexually transmitted infections in the European Union. Sex. Transm. Infect. 2004, 80:255-263.
6. Fleming D.T., Wasserheit J.N. From epidemiological synergy to public health policy and practice: the contribution of other sexually transmitted diseases to sexual transmission of HIV infection. Sex. Transm. Infect. 1999, 75:3-17.
7. Gao X., Chen X.S., Yin Y.P., Zhong M.Y., Shi M.Q., Wei W.H., Chen Q., Peeling R.W., Mabey D. Distribution study of Chlamydia trachomatis serovars among high-risk women in China performed using PCR-restriction fragment length polymorphism genotyping. J. Clin. Microbiol. 2007, 45:1185-1189.
8. Gundry C.N., Vandersteen J.G., Reed G.H., Pryor R.J., Chen J., Wittwer C.T. Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes. Clin. Chem. 2003, 49:396-406.
9. Jalal H., Stephen H., Alexander S., Carne C., Sonnex C. Development of real-time PCR assays for genotyping of Chlamydia trachomatis. J. Clin. Microbiol. 2007, 45:2649-2653.
10. Lima H.E., Oliveira M.B., Valente B.G., Afonso D.A., Darocha W.D., Souza M.C., Alvim T.C., Barbosa-Stancioli E.F., Noronha F.S. Genotyping of Chlamydia trachomatis from endocervical specimens in Brazil. Sex. Transm. Dis. 2007, 34:709-717.
11. Lin J.H., Tseng C.P., Chen Y.J., Lin C.Y., Chang S.S., Wu H.S., Cheng J.C. Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis. J. Clin. Microbiol. 2008, 46:1090-1097.
12. Lister N.A., Tabrizi S.N., Fairley C.K., Smith A., Janssen P.H., Garland S. Variability of the Chlamydia trachomatis omp1 gene detected in samples from men tested in male-only saunas in Melbourne, Australia. J. Clin. Microbiol. 2004, 42:2596-2601.
13. Molano M., Meijer C.J., Morré S.A., Pol R., van den Brule A.J. Combination of PCR targeting the VD2 of omp1 and reverse line blot analysis for typing of urogenital Chlamydia trachomatis serovars in cervical scrape specimens. J. Clin. Microbiol. 2004, 42:2935-2939.
14. Morré S.A., Ouburg S., van Agtmael M.A., de Vries H.J. Lymphogranuloma venereum diagnostics: from culture to real-time quadriplex polymerase chain reaction. Sex. Transm. Infect. 2008, 84:252-253.
15. Quint K.D., van Doorn L.J., Kleter B., de Koning M.N., van den Munckhof H.A., Morre S.A., ter Harmsel B., Weiderpass E., Harbers G., Melchers W.J., Quint W.G. A highly sensitive, multiplex broad-spectrum PCR-DNA-enzyme immunoassay and reverse hybridization assay for rapid detection and identification of Chlamydia trachomatis serovars. J. Mol. Diagn. 2007, 9:631-638.
16. Reed G.H., Wittwer C.T. Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clin. Chem. 2004, 50:1748-1754.
17. Robertson T., Bibby S., O'Rourke D., Belfiore T., Lambie H., Noormohammadi A.H. Characterization of Chlamydiaceae species using PCR and high resolution melt curve analysis of the 16S rRNA gene. J. Appl. Microbiol. 2009, 107:2017-2028.
18. Shank D., Jensen N., Wolfe D. A method to enumerate Chlamydia trachomatis by counting DFA stained elementary bodies on a printed microscope slide 1999.
19. Silins I., Ryd W., Strand A., Wadell G., Törnberg S., Hansson B.G., Wang X., Arnheim L., Dahl V., Bremell D., Persson K., Dillner J., Rylander E. Chlamydia trachomatis infection and persistence of human papillomavirus. Int. J. Cancer 2005, 116:110-115.
20. Stothard D.R. Use of a reverse dot blot procedure to identify the presence of multiple serovars in Chlamydia trachomatis urogenital infection. J. Clin. Microbiol. 2001, 39:2655-2659.
21. Tajiri-Utagawa E., Hara M., Takahashi K., Watanabe M., Wakita T. Development of a rapid high-throughput method for high-resolution melting analysis for routine detection and genotyping of noroviruses. J. Clin. Microbiol. 2009, 47:435-440.
22. Thylefors B., Négrel A.D., Pararajasegaram R., Dadzie K.Y. Global data on blindness. Bull. World Health Organ. 1995, 73:115-121.
23. van der Stoep N., van Paridon C.D., Janssens T., Krenkova P., Stambergova A., Macek M., Matthijs G., Bakker E. Diagnostic guidelines for high-resolution melting curve (HRM) analysis: an interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner. Hum. Mutat. 2009, 30:899-909.
24. WHO Global prevalence and incidence of selected curable sexually transmitted infections: overview and estimates 2001, World Health Organization Geneva, Switzerland.
25. Wittwer C.T. High-resolution DNA melting analysis: advancements and limitations. Hum.Mutat. 2009, 30:857-859.
26. Wittwer C.T., Reed G.H., Gundry C.N., Vandersteen J.G., Pryor R.J. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin. Chem. 2003, 49:853-860.
27. Yuan Y., Zhang Y.X., Watkins N.G., Caldwell H.D. Nucleotide and deduced amino acid sequences for the four variable domains of the major outer membrane proteins of the 15 Chlamydia trachomatis serovars. Infect. Immun. 1989, 57:1040-1049.
28. Zheng H.P., Jiang L.F., Fang D.Y., Xue Y.H., Wu Y.A., Huang J.M., Ou Z.Y. Application of an oligonucleotide array assay for rapid detecting and genotyping of Chlamydia trachomatis from urogenital specimens. Diagn. Microbiol. Infect. Dis. 2007, 57:1-6.
29. Zheng H.P., Jiang L.F., Fang D.Y., Wu X.Z., Li Y.W., Xu G., Xue Y.H., Feng Z.Q., Wu Y.A., Huang J.M. Genotyping of Chlamydia trachomatis by sequence analysis of VS1-VS2 region of omp1 gene. Chin J Dermatol 2007, 40:277-280.