Using neurogenetics and the warmth-gated ion channel TRPA1 to study the neural basis of behavior in Drosophila

Journal of Undergraduate Neuroscience Education

Volumn 9, Issue 1, 2010, Pages

  Berni, Jimena ^a   Muldal, Alistair M ^a   Pulver, Stefan R ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Behavior; Drosophila; GAL4 UAS system; Neurogenetics; Neuromuscular junction (NMJ); TRPA1

Indexed keywords

EID: 78649294998     PISSN: None     EISSN: 15442896     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Ainsley JA, Pettus JM, Bosenko D, Gerstein CE, Zinkevich N, Anderson MG, Adams CM, Welsh MJ, Johnson WA (2003) Enhanced locomotion caused by loss of the Drosophila DEG/ENaC protein Pickpocket1. Curr Biol 13:1557-1563.
2. Al-Anzi B, Tracey WD, Jr, Benzer S (2006) Response of Drosophila to wasabi is mediated by painless, the fly homolog of mammalian TRPA1/ANKTM1. Curr Biol 16:1034-1040.
3. Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K (2005) Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci 8:1263-1268.
4. Brand AH, Perrimon N (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development (Cambridge, England) 118:401-415.
5. Brent J, Werner K, McCabe BD (2009) Drosophila larval NMJ immunohistochemistry. J Vis Exp 25:1108.
6. Clyne JD, Miesenböck G (2008) Sex-specific control and tuning of the pattern generator for courtship song in Drosophila. Cell 133:354-363.
7. Gelbart ME, Kuroda MI (2009) Drosophila dosage compensation: a complex voyage to the X chromosome. Development 136:1399-1410
8. Hamada FN, Rosenzweig M, Kang K, Pulver SR, Ghezzi A, Jegla TJ, Garrity PA (2008) An internal thermal sensor controlling temperature preference in Drosophila. Nature 454:217-220.
9. Hazelrigg T, Mansfield JH (2006) Green fluorescent protein applications in Drosophila. Methods Biochem Anal 47:227-257.
10. Hornstein NJ, Pulver SR, Griffith LC (2009) Channelrhodopsin2 mediated stimulation of synaptic potentials at Drosophila neuromuscular junctions. J Vis Exp 25:1133.
11. Hwang RY, Zhong L, Xu Y, Johnson T, Zhang F, Deisseroth K, Tracey WD (2007) Nociceptive neurons protect Drosophila larvae from parasitoid wasps. Curr Biol 17:2105-2116.
12. Imlach W, McCabe BD (2009) Electrophysiological methods for recording synaptic potentials from the NMJ of Drosophila larvae. J Vis Exp 24:1109.
13. Jan LY, Jan YN (1976) Properties of the larval neuromuscular junction in Drosophila melanogaster. J Phyiol 262:189-214
14. Kitamoto T (2001) Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons. J Neurobiol 47:81-92.
15. Krans JL and Hoy RR (2005) Tools for physiology labs: An inexpensive means of temperature control. J Undergrad Neurosci Ed 4:A22-A26.
16. Krans JL, Rivlin PK, Hoy RR (2005) Demonstrating the Temperature Sensitivity of Synaptic Transmission in a Drosophila Mutant. J Undergrad Neurosci Ed 4:A27-A33.
17. Lott GK, Johnson BR, Bonow RH, Land BR, Hoy RR (2009) g-PRIME: A Free, Windows Based Data Acquisition and Event Analysis Software Package for Physiology in Classrooms and Research Labs. J Undergrad Neurosci Ed 8:A50-A54.
18. Meinertzhagen IA, Takemura SY, Lu Z, Huang S, Gao S, Ting CY, Lee CH (2009) From form to function: the ways to know a neuron. J Neurogenet 23:68-77.
19. Parisky KM, Agosto J, Pulver SR, Shang Y, Kuklin E, Hodge JJ, Kang K, Liu X, Garrity PA, Rosbash M, Griffith LC (2008) PDF cells are a GABA-responsive wake-promoting component of the Drosophila sleep circuit. Neuron 60:672-682.
20. Peabody NC, Pohl JB, Diao F, Vreede AP, Sandstrom DJ, Wang H, Zelensky PK, White BH (2009) Characterization of the decision network for wing expansion in Drosophila using targeted expression of the TRPM8 channel. J Neurosci 29:3343-3353.
21. Pulver SR, Pashkovski SL, Hornstein NJ, Garrity PA, Griffith LC (2009) Temporal dynamics of neuronal activation by Channelrhodopsin-2 and TRPA1 determine behavioral output in Drosophila larvae. J Neurophysiol 101:3075-3088.
22. Robinow S, White K (1991) Characterization and spatial distribution of the ELAV protein during Drosophila melanogaster development. J Neurobiol 22:443-461.
23. Rosenzweig M, Kang K, Garrity PA (2008) Distinct TRP channels are required for warm and cool avoidance in Drosophila melanogaster. Proc Natl Acad Sci U S A 105:14668-14673.
24. Rosenzweig M, Brennan KM, Tayler TD, Phelps PO, Patapoutian A, Garrity PA (2005) The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis. Genes Dev 19:419-424.
25. Schroll C, Riemensperger T, Bucher D, Ehmer J, Voller T, Erbguth K, Gerber B, Hendel T, Nagel G, Buchner E, Fiala A (2006) Light-induced activation of distinct modulatory neurons triggers appetitive or aversive learning in Drosophila larvae. Curr Biol 16:1741-1747.
26. Shang Y, Griffith LC, Rosbash M (2008) Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain. Proc Natl Acad Sci U S A 105:19587-19594.
27. Sun B, Xu P, Salvaterra PM (1999) Dynamic visualization of nervous system in live Drosophila. Proc Natl Acad Sci U S A 96:10438-10443.
28. Timmons L, Becker J, Barthmaier P, Fyrberg C, Shearn A, Fyrberg E (1997) Green fluorescent protein/beta-galactosidase double reporters for visualizing Drosophila gene expression patterns. Dev Genet 20:338-347.
29. Tracey WD Jr, Wilson RI, Laurent G, Benzer S (2003) painless, a Drosophila gene essential for nociception. Cell 113:261-273.
30. Wang J, Zugates CT, Liang IH, Lee CH, Lee T (2002) Drosophila Dscam is required for divergent segregation of sister branches and suppresses ectopic bifurcation of axons. Neuron 33:559-571.
31. Zhang W, Ge W, Wang Z (2007) A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons. Eur J Neurosci 26:2405-2416.