Effects of cryogenic preservation and storage on the molecular characteristics of microorganisms in sediments

Environmental Science and Technology

Volumn 44, Issue 21, 2010, Pages 8243-8247

  Brow, Christina N ^a   Johnson, Reid O'Brien ^a   Xu, Mouzhong ^a   Johnson, Richard L ^a   Simon, Holly M ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

16S RDNA; 16S RRNA; ARCHAEA; CELLULAR GROWTH RATE; ENRICHMENT CULTURE; GENE TRANSCRIPTS; HIGH RESOLUTION; IN-VIVO; MICROBIAL COMMUNITY COMPOSITION; MICROBIAL POPULATIONS; MOLECULAR CHARACTERISTICS; MOLECULAR CHARACTERIZATION; PCR-SSCP; PHYLOTYPES; QUANTITATIVE POLYMERASE CHAIN REACTION; RELATIVE ABUNDANCE; SEDIMENT SAMPLES; SINGLE-STRAND CONFORMATION POLYMORPHISM; SUBTILIS;

AQUIFERS; BACTERIA; BACTERIOLOGY; BIODEGRADATION; BIOREMEDIATION; BIOTECHNOLOGY; CRYOGENICS; FREEZING; GENES; POLLUTION; RNA; SEDIMENTOLOGY;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA; BACTERIAL RNA; DNA 16S; MESSENGER RNA; RIBOSOME RNA;

AQUIFER; BACTERIUM; BIOGEOCHEMISTRY; BIOREMEDIATION; CRYOPRESERVATION; FREEZING; GENETIC ANALYSIS; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION; SEDIMENT ANALYSIS; STORAGE;

ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; CRYOGENIC PRESERVATION; DEGRADATION; DNA EXTRACTION; FREEZING; GENETIC TRANSCRIPTION; GROWTH RATE; MICROBIAL DIVERSITY; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; PRESERVATION AND STORAGE; PSEUDOMONAS PUTIDA; QUANTITATIVE ANALYSIS; RNA EXTRACTION; SEDIMENT; SINGLE STRAND CONFORMATION POLYMORPHISM;

BACILLUS SUBTILIS; BIODEGRADATION, ENVIRONMENTAL; BIODIVERSITY; CRYOPRESERVATION; DNA, BACTERIAL; GENES, BACTERIAL; GEOLOGIC SEDIMENTS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; PSEUDOMONAS PUTIDA; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY;

BACTERIA (MICROORGANISMS);

EID: 78549265890     PISSN: 0013936X     EISSN: 15205851     Source Type: Journal    
DOI: 10.1021/es101641y     Document Type: Article

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