Multiplexing terbium-and europium-based TR-FRET readouts to increase kinase assay capacity

Journal of Biomolecular Screening

Volumn 15, Issue 8, 2010, Pages 1008-1015

  Horton, Robert A ^a   Vogel, Kurt W ^a  

^a Life Technologies   (United States)

Author keywords

homogeneous time resolved fluorescence (HTRF); kinase assay; mTOR; time resolved fluorescence energy transfer (TRET); TR FRET

Indexed keywords

2 (4 HYDROXYPHENYL) 4 MORPHOLINOPYRIDO[3',2':4,5]FURO[3,2 D]PYRIMIDINE; 4 (2 AMINOETHYLAMINO) 1,8 DIMETHYLIMIDAZO[1,2 A]QUINOXALINE; 5 (6 QUINOXALINYLMETHYLENE) 2,4 THIAZOLIDINEDIONE; EPIDERMAL GROWTH FACTOR RECEPTOR; ERLOTINIB; EUROPIUM; GEFITINIB; I KAPPA B KINASE BETA; I KAPPA B KINASE INHIBITOR; LY 249002; MAMMALIAN TARGET OF RAPAMYCIN; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHOTRANSFERASE INHIBITOR; STAUROSPORINE; TERBIUM; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DOSE RESPONSE; DRUG IDENTIFICATION; DRUG POTENCY; DRUG SCREENING; ENZYME ASSAY; FLUORESCENCE RESONANCE ENERGY TRANSFER; IC 50; PRIORITY JOURNAL;

ANIMALS; CALIBRATION; DRUG EVALUATION, PRECLINICAL; ENZYME ASSAYS; EUROPIUM; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; I-KAPPA B KINASE; PHOSPHATIDYLINOSITOL 3-KINASES; PROTEIN KINASE INHIBITORS; RECEPTOR, EPIDERMAL GROWTH FACTOR; TERBIUM; TOR SERINE-THREONINE KINASES;

EID: 78449273901     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057110368993     Document Type: Article

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