Recombinant production of omega-3 fatty acids in Escherichia coli using a gene cluster isolated from Shewanella baltica MAC1

Journal of Applied Microbiology

Volumn 109, Issue 6, 2010, Pages 1897-1905

  Amiri Jami, M ^a,b   Griffiths, M W ^a,b  

^a UNIVERSITY OF GUELPH   (Canada)

^b UNIVERSITY OF GUELPH   (Canada)

Author keywords

Docosahexaenoic acid; Eicosapentaenoic acid; Omega 3 fatty acid; Omega 3 gene cluster

Indexed keywords

AGRICULTURAL PRODUCTS; BIOCHEMISTRY; BIOSYNTHESIS; CLONING; COST EFFECTIVENESS; ESCHERICHIA COLI; GAS CHROMATOGRAPHY; MASS SPECTROMETRY;

DOCOSAHEXAENOIC ACID; E. COLI; EICOSAPENTAENOIC ACID; GENE CLUSTERS; OMEGA-3; OMEGA-3 GENE CLUSTER; OMEGA-3-FATTY ACIDS; OPEN READING FRAME; SHEWANELLA; TRANSGENICS;

GENES;

DOCOSAHEXAENOIC ACID; ICOSAPENTAENOIC ACID; OMEGA 3 FATTY ACID;

BACTERIUM; COLIFORM BACTERIUM; COST-BENEFIT ANALYSIS; FATTY ACID; FERMENTATION; GAS CHROMATOGRAPHY; GENE; HYBRIDIZATION; MARINE ECOSYSTEM; MASS SPECTROMETRY;

AGRICULTURE; ARTICLE; BACTERIAL GENE; DNA HYBRIDIZATION; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; FERMENTATION; GAS CHROMATOGRAPHY; GENE CLUSTER; GENE ISOLATION; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PFAA GENE; PFAB GENE; PFAC GENE; PFAD GENE; PROTEIN DOMAIN; SEQUENCE HOMOLOGY; SHEWANELLA; SHEWANELLA BALTICA;

ESCHERICHIA COLI; SHEWANELLA BALTICA;

EID: 78449238510     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04817.x     Document Type: Article

References (23)

1. Allen, E.E. and Bartlett, D.H. (2002) Structure and regulation of the omega-3 polyunsaturated fatty acid synthase genes from the deep-sea bacterium Photobacterium profundum strain SS9. Microbiology 148, 1903-1913.
2. Amiri-Jami, M., Wang, H., Kakuda, Y. and Griffiths, M.W. (2006) Enhancement of polyunsaturated fatty acid production by Tn5 transposon in Shewanella baltica. Biotechnol Lett 28, 1187-1192.
3. Antalis, C.J., Arnold, T., Lee, B., Buhman, K.K. and Siddiqui, R.A. (2009) Docosahexaenoic acid is a substrate for ACAT1 and inhibits cholesteryl ester formation from oleic acid in MCF-10A cells. Prostaglandins Leukot Essent Fatty Acids 80, 165-171.
4. Barclay, W.R., Meager, K.M. and Abril, J.R. (1994) Heterotrophic production of long chain omega-3 fatty acids utilizing algae and algae-like microorganisms. J Appl Phycol 6, 123-129.
5. Barclay, W., Abril, R., Abril, P., Weaver, C. and Ashford, A. (1998) Production of docosahexaenoic acid from microalgae and its benefits for use in animal feeds. World Rev Nutr Diet 83, 61-76.
6. Bourre, J.M. (2007) Dietary omega-3 fatty acids for women. Biomed Pharmacother 61, 105-112.
7. Cadieux, S., Kakuda, Y., Ward, O. and Griffiths, M. (1998) Bacterial production of omega-3 fatty acids in whey. Milchwissenschaft 53, 547-551.
8. Chi, Z., Liu, Y., Frear, C. and Chen, S. (2008) Study of a two-stage growth of DHA-producing marine algae Schizochytrium limacinum SR21 with shifting dissolved oxygen level. Appl Microbiol Biotechnol 81, 1141-1148.
9. Jiang, H., Zirkle, R., Metz, J.G., Braun, L., Richter, L., Van Lanen, S.G. and Shen, B. (2008) The role of tandem acyl carrier protein domains in polyunsaturated fatty acid biosynthesis. J Am Chem Soc 130, 6336-6337.
10. La Guardia, M., Giammanco, S., Di Majo, D., Tabacchi, G., Tripoli, E. and Giammanco, M. (2005) Omega 3 fatty acids: biological activity and effects on human health. Panminerva Med 47, 245-257.
11. Metz, J.G., Roessler, P., Facciotti, D., Levering, C., Dittrich, F., Lassner, M., Valentine, R., Lardizabal, K. et al. (2001) Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 293, 290-293.
12. Morita, N., Tanaka, M. and Okuyama, H. (2000) Biosynthesis of fatty acids in the docosahexaenoic acid-producing bacterium Moritella marina strain MP-1. Biochem Soc Trans 28, 943-945.
13. Okuyama, H., Orikasa, Y., Nishida, T., Watanabe, K. and Morita, N. (2007) Bacterial genes responsible for the biosynthesis of eicosapentaenoic and docosahexaenoic acids and their heterologous expression. Appl Environ Microbiol 73, 665-670.
14. Orikasa, Y., Yamada, A., Yu, R., Ito, Y., Nishida, T., Yumoto, I., Watanabe, K. and Okuyama, H. (2004) Characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. strain SCRC-2738. Cell Mol Biol (Noisy-le-grand) 50, 625-630.
15. Orikasa, Y., Nishida, T., Hase, A., Watanabe, K., Morita, N. and Okuyama, H. (2006a) A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1. FEBS Lett 580, 4423-4429.
16. Orikasa, Y., Nishida, T., Yamada, A., Yu, R., Watanabe, K., Hase, A., Morita, N. and Okuyama, H. (2006b) Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli. Biotechnol Lett 28, 1841-1847.
17. Orikasa, Y., Tanaka, M., Sugihara, S., Hori, R., Nishida, T., Ueno, A., Morita, N., Yano, Y. et al. (2009) pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis. FEMS Microbiol Lett 295, 170-176.
18. Siddiqui, R.A., Harvey, K. and Stillwell, W. (2008) Anticancer properties of oxidation products of docosahexaenoic acid. Chem Phys Lipids 153, 47-56.
19. Sijtsma, L. and De Swaaf, M.E. (2004) Biotechnological production and applications of the omega-3 polyunsaturated fatty acid docosahexaenoic acid. Appl Microbiol Biotechnol 64, 146-153.
20. Sugihara, S., Orikasa, Y. and Okuyama, H. (2008) An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid. Biotechnol Lett 30, 411-414.
21. Swinbanks, D. (1993) Fish-oil miracle additive brings benefits to some. Nature 364, 180.
22. Tanaka, M., Ueno, K., Kawasaki, K., Yumoto, I., Ohgiya, S., Hoshino, T., Ishizaki, K., Okuyama, H. et al. (1999) Isolated of clustered genes that are notably homologous to the eicosapentaenoic acid biosynthesis gene cluster from the docosahexaenoic acid-producing bacterium Vibrio marinus strain MP-1. Biotechnol Lett 21, 939-945.
23. Yazawa, K. (1996) Production of eicosapentaenoic acid from marine bacteria. Lipids 31(Suppl), S297-S300.