Bacterial colonization of colonic crypt mucous gel and disease activity in ulcerative colitis

Annals of Surgery

Volumn 252, Issue 5, 2010, Pages 869-874

  Rowan, Fiachra ^a   Docherty, Neil G ^b   Murphy, Madeline ^a   Murphy, T Brendan ^c   Coffey, J Calvin ^a   O'Connell, P Ronan ^a  

^a ST VINCENT S UNIVERSITY HOSPITAL   (Ireland)

^b TRINITY COLLEGE DUBLIN   (Ireland)

^c UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; AZATHIOPRINE; BACTERIAL RNA; CORTICOSTEROID DERIVATIVE; CYCLOSPORIN; INFLIXIMAB; MYCOPHENOLIC ACID; RIBOSOME RNA; RNA 16S;

ACUTE DISEASE; ADULT; AGED; ASCENDING COLON; BACTERIAL COLONIZATION; CLINICAL ARTICLE; COLON BIOPSY; COLON MUCOSA; CONFERENCE PAPER; CONTROLLED STUDY; COPY NUMBER VARIATION; CRYPT ASSOCIATED MUCOUS GEL; DISEASE ACTIVITY; FEMALE; GENE LOCATION; HUMAN; LASER CAPTURE MICRODISSECTION; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SIGMOID; TRANSVERSE COLON; ULCERATIVE COLITIS; COLON RESECTION; COLONIC CRYPT MUCOUS GEL; DESULFOVIBRIO DESULFURICANS; GEL; QUANTITATIVE ANALYSIS;

ADULT; AGED; ANALYSIS OF VARIANCE; AREA UNDER CURVE; CASE-CONTROL STUDIES; COLITIS, ULCERATIVE; DESULFOVIBRIO; DNA, BACTERIAL; FEMALE; HUMANS; INTESTINAL MUCOSA; LASERS; MALE; MICRODISSECTION; MIDDLE AGED; MUCUS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL; SENSITIVITY AND SPECIFICITY; STATISTICS, NONPARAMETRIC;

EID: 78349281542     PISSN: 00034932     EISSN: 15281140     Source Type: Journal    
DOI: 10.1097/SLA.0b013e3181fdc54c     Document Type: Conference Paper

References (24)

1. Cummings JH, Macfarlane GT, Macfarlane S. Intestinal bacteria and ulcerative colitis. Curr Issues Intest Microbiol. 2003;4(1):9-20.
2. Macfarlane S, Furrie E, Cummings JH, et al. Chemotaxonomic analysis of bacterial populations colonizing the rectal mucosa in patients with ulcerative colitis. Clin Infect Dis. 2004;38(12):1690-1699.
3. Gill SR, Pop M, Deboy RT, et al. Metagenomic analysis of the human distal gutmicrobiome. Science. 2006;312(5778):1355-1359.
4. Shanahan F. Host-flora interactions in inflammatory bowel disease. Inflamm Bowel Dis. 2004;10(suppl 1):S16-S24.
5. Macdonald TT, Monteleone G. Immunity inflammation, and allergy in the gut. Science. 2005;307(5717):1920-1925.
6. Swidsinski A, Loening-Baucke V, Lochs H, et al. Spatial organization ofbacte-rial flora in normal and inflamed intestine: a fluorescence in situ hybridization studyinmice. World J Gastroenterol. 2005;11(8):1131-1140.
7. Swidsinski A, Weber J, Loening-Baucke V, et al. Spatial organization and composition of the mucosal flora in patients with inflammatory bowel disease. J Clin Microbiol. 2005;43(7):3380-3389.
8. Wilson M. Bacterial biofilms and human disease. Sci Prog. 2001;84(pt 3): 235-254.
9. Hartley CL, Neumann CS, Richmond MH. Adhesion ofcommensal bacteria to the large intestine wall in humans. Infect Immun. 1979;23(1):128-132.
10. De Hertogh G, Aerssens J, de Hoogt R, et al. Validation of 16s DNA sequenc-ing in microdissected bowel biopsies from Crohn's disease patients to assess bacterialfloradiversity J Pathol. 2006;209(4):532-539.
11. Nadkarni MA, Martin FE, Jacques NA, et al. Determination ofbacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology. 2002;148(pt 1):257-266.
12. Fite A, Macfarlane GT, Cummings JH, et al. Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction. Gut. 2004;53(4):523-529.
13. Laird NM, Ware JH. Random-effects models for longitudinal data. Biometrics. 1982;38(4):963-974.
14. Cnaan A, Laird NM, Slasor P. Using the general linear mixed model to analyse unbalanced repeated measures and longitudinal data. Stat Med. 1997;16(20):2349-2380.
15. Swidsinski A, Loening-Baucke V, Theissig F, et al. Comparative study of the intestinal mucus barrier in normal and inflamed colon. Gut. 2007;56(3): 343-350.
16. Acinas SG, Marcelino LA, Klepac-Ceraj V, et al. Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons. J Bacteriol. 2004;186(9):2629-2635.
17. Hattori M, Taylor TD. The human intestinal microbiome: a new frontier of humanbiology DNA Res. 2009;16(1):1-12.
18. Johansson ME, Phillipson M, Petersson J, et al. The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria. Proc Natl Acad Sci USA. 2008;105(39):15064-15069.
19. Hopkins MJ, Macfarlane GT, Furrie E, et al. Characterisation ofintestinal bac-teria in infant stools using real-time PCR and northern hybridisation analyses. FEMS Microbiol Ecol. 2005;54(1):77-85.
20. Woodmansey EJ, McMurdo ME, Macfarlane GT, et al. Comparison of com-positions and metabolic activities of fecal microbiotas in young adults and in antibiotic-treated and non-antibiotic-treated elderly subjects. Appl Environ Microbiol. 2004;70(10):6113-6122.
21. Schultsz C, Van Den Berg FM, Ten Kate FW, et al. The intestinal mucus layer from patients with inflammatory bowel disease harbors high numbers of bacteria compared with controls. Gastroenterology. 1999;117(5):1089-1097.
22. Ott SJ, Musfeldt M, Ullmann U, et al. Quantification of intestinal bacte-rial populations by real-time PCR with a universal primer set and minor groove binder probes: a global approach to the enteric flora. J Clin Micro-biol. 2004;42(6):2566-2572.
23. Tsukada Y, Nakamura T, Iimura M, et al. Cytokine profile in colonic mucosa ofulcerative colitis correlates with disease activity and response to granulo-cytapheresis. Am J Gastroenterol. 2002;97(11):2820-2828.
24. Xavier RJ, Podolsky DK. Unravelling the pathogenesis ofinflammatory bowel disease. Nature. 2007;448(7152):427-434.