Methods to quantify sex steroid hormones in bone: Applications to the study of androgen ablation and administration

American Journal of Physiology - Endocrinology and Metabolism

Volumn 299, Issue 5, 2010, Pages

  Yarrow, Joshua F ^a,b   Conover, Christine F ^a   Lipinska, Judyta A ^a   Santillana, Cesar A ^a   Wronski, Thomas J ^b   Borst, Stephen E ^a,b  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

Dihydrotestosterone; Estradiol; Estrogen; Intracrine; Testosterone

Indexed keywords

ANDROGEN; ANDROSTANOLONE; ESTRADIOL; SEX HORMONE; TESTOSTERONE; TESTOSTERONE ENANTATE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BONE; HORMONE BLOOD LEVEL; IMMUNOASSAY; MALE; MUSCLE LEVEL; NONHUMAN; ORCHIECTOMY; PRIORITY JOURNAL; RAT; TIBIA; ANIMAL; CHEMISTRY; COMPARATIVE STUDY; COMPUTER ASSISTED TOMOGRAPHY; FISCHER 344 RAT; GERMFREE ANIMAL; PILOT STUDY; ULTRASTRUCTURE; VALIDATION STUDY;

ANIMALS; BONE AND BONES; DIHYDROTESTOSTERONE; ESTRADIOL; MALE; PILOT PROJECTS; RATS; RATS, INBRED F344; SPECIFIC PATHOGEN-FREE ORGANISMS; TESTOSTERONE; TOMOGRAPHY, X-RAY COMPUTED;

EID: 78349270871     PISSN: 01931849     EISSN: 15221555     Source Type: Journal    
DOI: 10.1152/ajpendo.00384.2010     Document Type: Article

References (28)

1. Amory JK, Anawalt BD, Matsumoto AM, Page ST, Bremner WJ, Wang C, Swerdloff RS, Clark RV. The effect of 5alpha-reductase inhibition with dutasteride and finasteride on bone mineral density, serum lipoproteins, hemoglobin, prostate specific antigen and sexual function in healthy young men. J Urol 179: 2333-2338, 2008.
2. Amory JK, Watts NB, Easley KA, Sutton PR, Anawalt BD, Matsumoto AM, Bremner WJ, Tenover JL. Exogenous testosterone or testosterone with finasteride increases bone mineral density in older men with low serum testosterone. J Clin Endocrinol Metab 89: 503-510, 2004.
3. Bauer ER, Daxenberger A, Petri T, Sauerwein H, Meyer HH. Characterisation of the affinity of different anabolics and synthetic hormones to the human androgen receptor, human sex hormone binding globulin and to the bovine progestin receptor. APMIS 108: 838-846, 2000.
4. Borst SE, Conover CF, Carter CS, Gregory CM, Marzetti E, Leeuwenburgh C, Vandenborne K, Wronski TJ. Anabolic effects of testosterone are preserved during inhibition of 5α-reductase. Am J Physiol Endocrinol Metab 293: E507-E514, 2007.
5. Borst SE, Lee JH, Conover CF. Inhibition of 5α-reductase blocks prostate effects of testosterone without blocking anabolic effects. Am J Physiol Endocrinol Metab 288: E222-E227, 2005.
6. Carani C, Qin K, Simoni M, Faustini-Fustini M, Serpente S, Boyd J, Korach KS, Simpson ER. Effect of testosterone and estradiol in a man with aromatase deficiency. N Engl J Med 337: 91-95, 1997.
7. Compston JE. Sex steroids and bone. Physiol Rev 81: 419-447, 2001.
8. Eyre LJ, Bland R, Bujalska IJ, Sheppard MC, Stewart PM, Hewison M. Characterization of aromatase and 17 beta-hydroxysteroid dehydrogenase expression in rat osteoblastic cells. J Bone Miner Res 13: 996-1004, 1998.
9. Falahati-Nini A, Riggs BL, Atkinson EJ, O'Fallon WM, Eastell R, Khosla S. Relative contributions of testosterone and estrogen in regulating bone resorption and formation in normal elderly men. J Clin Invest 106: 1553-1560, 2000.
10. Gennari L, Nuti R, Bilezikian JP. Aromatase activity and bone homeostasis in men. J Clin Endocrinol Metab 89: 5898-5907, 2004.
11. Labrie F, Luu-The V, Lin SX, Simard J, Labrie C. Role of 17 beta-hydroxysteroid dehydrogenases in sex steroid formation in peripheral intracrine tissues. Trends Endocrinol Metab 11: 421-427, 2000.
12. Lindberg MK, Alatalo SL, Halleen JM, Mohan S, Gustafsson JA, Ohlsson C. Estrogen receptor specificity in the regulation of the skeleton in female mice. J Endocrinol 171: 229-236, 2001.
13. Matsumoto AM, Tenover L, McClung M, Mobley D, Geller J, Sullivan M, Grayhack J, Wessells H, Kadmon D, Flanagan M, Zhang GK, Schmidt J, Taylor AM, Lee M, Waldstreicher J. The long-term effect of specific type II 5alpha-reductase inhibition with finasteride on bone mineral density in men: results of a 4-year placebo controlled trial. J Urol 167: 2105-2108, 2002.
14. Muir M, Romalo G, Wolf L, Elger W, Schweikert HU. Estrone sulfate is a major source of local estrogen formation in human bone. J Clin Endocrinol Metab 89: 4685-4692, 2004.
15. Murata Y, Robertson KM, Jones ME, Simpson ER. Effect of estrogen deficiency in the male: the ArKO mouse model. Mol Cell Endocrinol 193: 7-12, 2002.
16. Ohlsson C, Vandenput L. The role of estrogens for male bone health. Eur J Endocrinol 160: 883-889, 2009.
17. Oz OK, Zerwekh JE, Fisher C, Graves K, Nanu L, Millsaps R, Simpson ER. Bone has a sexually dimorphic response to aromatase deficiency. J Bone Miner Res 15: 507-514, 2000.
18. Rochira V, Zirilli L, Madeo B, Aranda C, Caffagni G, Fabre B, Montangero VE, Roldan EJ, Maffei L, Carani C. Skeletal effects of long-term estrogen and testosterone replacement treatment in a man with congenital aromatase deficiency: evidences of a priming effect of estrogen for sex steroids action on bone. Bone 40: 1662-1668, 2007.
19. Sillat T, Pöllänen R, Lopes JR, Porola P, Ma G, Korhonen M, Konttinen YT. Intracrine androgenic apparatus in human bone marrow stromal cells. J Cell Mol Med 13: 3296-3302, 2009.
20. van der Eerden BC, Löwik CW, Wit JM, Karperien M. Expression of estrogen receptors and enzymes involved in sex steroid metabolism in the rat tibia during sexual maturation. J Endocrinol 180: 457-467, 2004.
21. Van Der Eerden BC, Van De Ven J, Lowik CW, Wit JM, Karperien M. Sex steroid metabolism in the tibial growth plate of the rat. Endocrinology 143: 4048-4055, 2002.
22. Vandenput L, Ohlsson C. Estrogens as regulators of bone health in men. Nat Rev Endocrinol 5: 437-443, 2009.
23. Vanderschueren D, Boonen S, Ederveen AG, de Coster R, Van Herck E, Moermans K, Vandenput L, Verstuyf A, Bouillon R. Skeletal effects of estrogen deficiency as induced by an aromatase inhibitor in an aged male rat model. Bone 27: 611-617, 2000.
24. Vanderschueren D, Van Herck E, De Coster R, Bouillon R. Aromatization of androgens is important for skeletal maintenance of aged male rats. Calcif Tissue Int 59: 179-183, 1996.
25. Vanderschueren D, Vandenput L, Boonen S, Lindberg MK, Bouillon R, Ohlsson C. Androgens and bone. Endocr Rev 25: 389-425, 2004.
26. Vidal O, Lindberg MK, Hollberg K, Baylink DJ, Andersson G, Lubahn DB, Mohan S, Gustafsson JA, Ohlsson C. Estrogen receptor specificity in the regulation of skeletal growth and maturation in male mice. Proc Natl Acad Sci USA 97: 5474-5479, 2000.
27. Wronski TJ, Cintron M, Doherty AL, Dann LM. Estrogen treatment prevents osteopenia and depresses bone turnover in ovariectomized rats. Endocrinology 123: 681-686, 1988.
28. Yarrow JF, Conover CF, Purandare AV, Bhakta AM, Zheng N, Conrad B, Altman MK, Franz SE, Wronski TJ, Borst SE. Supraphysiological testosterone enanthate administration prevents bone loss and augments bone strength in gonadectomized male and female rats. Am J Physiol Endocrinol Metab 295: E1213-E1222, 2008.