One-step split GFP staining for sensitive protein detection and localization in mammalian cells

BioTechniques

Volumn 49, Issue 4, 2010, Pages 727-736

  Kaddoum, Lara ^a,c   Magdeleine, Eddy ^a,c   Waldo, Geoffrey S ^d   Joly, Etienne ^a,c   Cabantous, Stéphanie ^b,c  

^a INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^b INSTITUT CLAUDIUS REGAUD   (France)

^c UNIVERSITÉ DE TOULOUSE   (France)

^d LOS ALAMOS NATIONAL LABORATORY   (United States)

Author keywords

Epitope tagging; Green fluorescent protein; Intracellular staining; Protein fragment complementation; Protein localization; Split GFP

Indexed keywords

AMINO ACID; GREEN FLUORESCENT PROTEIN; PROTEIN TAG;

ARTICLE; CELLULAR DISTRIBUTION; EMBRYO; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MAMMAL CELL; MICROSCOPY; PROTEIN DETERMINATION; SIGNAL NOISE RATIO; SIGNAL TRANSDUCTION; STAINING;

ANIMALS; CELL LINE, TUMOR; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; INTRACELLULAR SPACE; MICE; MICROSCOPY, FLUORESCENCE; RECOMBINANT FUSION PROTEINS;

MAMMALIA;

EID: 78349239240     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113512     Document Type: Article

References (20)

1. Tsien, R.Y. 1998. The green fluorescent protein. Annu. Rev. Biochem. 67:509-544.
2. Hanson, D.A. and S.F. Ziegler. 2004. Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule. J. Negat. Results Biomed. 3:2.
3. Brizzard, B. 2008. Epitope tagging. BioTechniques 44:693-695.
4. Krenacs, T., L. Krenacs, and M. Raffeld. 1999. Multiple antigen immunostaining procedures. Methods Mol. Biol. 115:223-233.
5. Evan, G.I., G.K. Lewis, G. Ramsay, and J.M. Bishop. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5:3610-3616.
6. Cabantous, S., J.D. Pedelacq, B.L. Mark, C. Naranjo, T.C. Terwilliger, and G.S. Waldo. 2005. Recent advances in GFP folding reporter and split-GFP solubility reporter technologies. Application to improving the folding and solubility of recalcitrant proteins from Mycobacterium tuberculosis. J. Struct. Funct. Genomics 6:113-119.
7. Cabantous, S. and G.S. Waldo. 2006. In vivo and in vitro protein solubility assays using split GFP. Nat. Methods 3:845-854.
8. Mnatzakanian, G.N., H. Lohi, I. Munteanu, S.E. Alfred, T. Yamada, P.J. MacLeod, J.R. Jones, S.W. Scherer, et al. 2004. A previously unidentified MECP2 open reading frame defines a new protein isoform relevant to Rett syndrome. Nat. Genet. 36:339-341.
9. Choy, E., V.K. Chiu, J. Silletti, M. Feoktistov, T. Morimoto, D. Michaelson, I.E. Ivanov, and M.R. Philips. 1999. Endomembrane trafficking of ras: the CAAX motif targets proteins to the ER and Golgi. Cell 98:69-80.
10. Skene, P.J., R.S. Il l ingwor th, S. Webb, A.R. Kerr, K.D. James, D.J. Turner, R. Andrews, and A.P. Bird. 2010. Neuronal MeCP2 is expressed at near histone-octamer levels and globally alters the chromatin state. Mol. Cell 37:457-468.
11. Young, J.I., E.P. Hong, J.C. Castle, J. Crespo- Barreto, A.B. Bowman, M.F. Rose, D. Kang, R. Richman, et al. 2005. Regulation of RNA splicing by the methylation-dependent transcriptional repressor methyl-CpG binding protein 2. Proc. Natl. Acad. Sci. USA 102:17551-17558.
12. Willumsen, B.M., A. Christensen, N.L. Hubbert, A.G. Papageorge, and D.R. Lowy. 1984. The p21 ras C-terminus is required for transformation and membrane association. Nature 310:583-586.
13. Wang, T. and P.K. Donahoe. 2004. The immunophilin FKBP12: a molecular guardian of the TGF-beta family type I receptors. Front. Biosci. 9:619-631.
14. Marchi, M., A. Guarda, A. Bergo, N. Landsberger, C. Kilstrup-Nielsen, G.M. Ratto, and M. Costa. 2007. Spatio-temporal dynamics and localization of MeCP2 and pathological mutants in living cells. Epigenetics 2:187-197.
15. Akbarian, S., R.Z. Chen, J. Gribnau, T.P. Rasmussen, H. Fong, R. Jaenisch, and E.G. Jones. 2001. Expression pattern of the Rett syndrome gene MeCP2 in primate prefrontal cortex. Neurobiol. Dis. 8:784-791.
16. De Angelis, D.A., G. Miesenbock, B.V. Zemelman, and J.E. Rothman. 1998. PRIM: proximity imaging of green fluorescent protein-tagged polypeptides. Proc. Natl. Acad. Sci. USA 95:12312-12316.
17. Zheng, H., J. McKay, and J.E. Buss. 2007. H-Ras does not need COP I- or COP II-dependent vesicular transport to reach the plasma membrane. J. Biol. Chem. 282:25760-25768.
18. Van Engelenburg, S.B. and A.E. Palmer. 2010. Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors. Nat. Methods 7:325-330.
19. Shevtsova, Z., J.M. Malik, U. Michel, U. Scholl, M. Bahr, and S. Kugler. 2006. Evaluation of epitope tags for protein detection after in vivo CNS gene transfer. Eur. J. Neurosci. 23:1961-1969.
20. Lobbestael, E., V. Reumers, A. Ibrahimi,K. Paesen, I. Thiry, R. Gijsbers, C. Van den Haute, Z. Debyser, et al. 2010. Immunohistochemical detection of transgene expression in the brain using small epitope tags. BMC Biotechnol. 10:16.