Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule

Journal of Negative Results in BioMedicine

Volumn 3, Issue , 2004, Pages

  Hanson, Dennis A ^a   Ziegler, Steven F ^b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b BENAROYA RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GRANULYSIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL GRANULE; CELLULAR DISTRIBUTION; COMPARATIVE STUDY; CYTOTOXIC T LYMPHOCYTE; LYMPHOCYTE ACTIVATION; LYMPHOCYTE MIGRATION; LYMPHOCYTE PROLIFERATION; NATURAL KILLER CELL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; SECRETORY CELL;

ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE; ARTIFACTS; GREEN FLUORESCENT PROTEINS; HUMANS; KILLER CELLS, NATURAL; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS;

EID: 5444244364     PISSN: 14775751     EISSN: None     Source Type: Journal    
DOI: 10.1186/1477-5751-3-2     Document Type: Article

References (7)

1. Patterson GH, Knobel SM, Sharif WD, Kain SR, Piston DW: Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys J 1997, 73:2782-2790.
2. Kaether C, Salm T, Glombik M, Almers W, Gerdes HH: Targeting of green fluorescent protein to neuroendocrine secretory granules: a new tool for real time studies of regulated protein secretion. Eur J Cell Biol 1997, 74:133-142.
3. Russell JH, Ley TJ: Lymphocyte-mediated Cytotoxicity. Ann Rev Immunol 2002, 20:323-370.
4. Page LJ, Darmon AJ, Uellner R, Griffiths GM: L is for lytic granules: lysosomes that kill. Biochim Biophys Acta 1998, 1401:146-156.
5. Pena SV, Hanson DA, Carr BA, Goralski TJ, Krensky AM: Processing, subcellular localization, and function of 519 (granulysin), a human late T cell activation molecule with homology to small, lytic, granule proteins. J Immunol 1997, 158:2680-2688.
6. Hanson DA, Kaspar AA, Poulain FR, Krensky AM: Biosynthesis of granulysin, a novel cytolytic molecule. Mol Immunol 1999, 36:413-422.
7. Yodoi J, Teshigawara K, Nikaido T, Fukui K, Noma T, Honjo T, Takigawa M, Sasaki M, Minato N, Tsudo M: TCGF (IL 2)-receptor inducing factor(s). I. Regulation of IL 2 receptor on a natural killer-like cell line (YT cells). J Immunol 1985, 134:1623-1630.