Simplified screening method for detecting oseltamivir resistant pandemic influenza A (H1N1) 2009 virus by a RT-PCR/restriction fragment length polymorphism assay

Journal of Virological Methods

Volumn 170, Issue 1-2, 2010, Pages 165-168

  Nukiwa, Nao ^a   Suzuki, Akira ^a   Furuse, Yuki ^a   Shimabukuro, Kozue ^a   Odagiri, Takashi ^a   Khandaker, Irona ^a   Oshitani, Hitoshi ^a  

^a TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

H275Y; Influenza; Oseltamivir; Pandemic; Resistance; RT PCR RFLP

Indexed keywords

HISTIDINE; SIALIDASE; TYROSINE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; INFLUENZA VIRUS A H1N1; NONHUMAN; PANDEMIC INFLUENZA VIRUS A H1N1 2009; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCREENING; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS MUTATION; VIRUS RESISTANCE; VIRUS TRANSMISSION;

AMINO ACID SUBSTITUTION; ANTIVIRAL AGENTS; DRUG RESISTANCE, VIRAL; HUMANS; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA, HUMAN; MICROBIAL SENSITIVITY TESTS; NEURAMINIDASE; OSELTAMIVIR; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

EID: 78249289654     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.09.005     Document Type: Article

References (30)

1. Carr M.J., Sayre N., Duffy M., Connell J., Hall W.W. Rapid molecular detection of the H275Y oseltamivir resistance gene mutation in circulating influenza A (H1N1) viruses. J. Virol. Methods 2008, 153:257-262.
2. Centers for Disease Control Prevention Outbreak of swine-origin influenza A (H1N1) virus infection-Mexico, March-April 2009. Morb. Mortal. Wkly. Rep. 2009, 58:467-470.
3. Center for Disease Control Prevention Update: drug susceptibility of swine-origin influenza A (H1N1) viruses, April 2009. Morb. Mortal. Wkly. Rep. 2009, 58:433-435.
4. Centers for Disease Control Prevention Patients hospitalized with 2009 pandemic influenza A (H1N1)-New York City, May 2009. Morb. Mortal. Wkly. Rep. 2010, 58:1436-1440.
5. Deyde V.M., Okomo-Adhiambo M., Sheu T.G., Wallis T.R., Fry A., Dharan N., Klimov A.I., Gubareva L.V. Pyrosequencing as a tool to detect molecular markers of resistance to neuraminidase inhibitors in seasonal influenza A viruses. Antiviral Res. 2009, 81:16-24.
6. Deyde V.M., Sheu T.G., Trujillo A.A., Okomo-Adhiambo M., Garten R., Klimov A.I., Gubareva L.V. Detection of molecular markers of drug resistance in 2009 pandemic influenza A (H1N1) viruses by pyrosequencing. Antimicrob. Agents Chemother. 2009, 54:1102-1110.
7. Gubareva L.V., Webster R.G., Hayden F.G. Detection of influenza virus resistance to neuraminidase inhibitors by an enzyme inhibition assay. Antiviral Res. 2002, 53:47-61.
8. Guo L., Garten R.J., Foust A.S., Sessions W.M., Okomo-Adhiambo M., Gubareva L.V., Klimov A.I., Xu X. Rapid identification of oseltamivir-resistant influenza A(H1N1) viruses with H274Y mutation by RT-PCR/restriction fragment length polymorphism assay. Antiviral Res. 2009, 82:29-33.
9. Hayden F.G., Cote K.M., Douglas R.G. Plaque inhibition assay for drug susceptibility testing of influenza viruses. Antimicrob. Agents Chemother. 1980, 17:865-870.
10. Hoffmann E., Stech J., Guan Y., Webster R.G., Perez D.R. Universal primer set for the full-length amplification of all influenza A viruses. Arch. Virol. 2001, 146:2275-2289.
11. Hurt A.C., Holien J.K., Parker M.W., Barr I.G. Oseltamivir resistance and the H274Y neuraminidase mutation in seasonal, pandemic and highly pathogenic influenza viruses. Drugs 2009, 69:2523-2531.
12. Jain S., Kamimoto L., Bramley A.M., Schmitz A.M., Benoit S.R., Louie J., Sugerman D.E., Druckenmiller J.K., Ritger K.A., Chugh R., Jasuja S., Deutscher M., Chen S., Walker J.D., Duchin J.S., Lett S., Soliva S., Wells E.V., Swerdlow D., Uyeki T.M., Fiore A.E., Olsen S.J., Fry A.M., Bridges C.B., Finelli L. Hospitalized patients with 2009 H1N1 influenza in the United States, April-June 2009. N. Engl. J. Med. 2009, 361:1935-1944.
13. Jamieson D.J., Honein M.A., Rasmussen S.A., Williams J.L., Swerdlow D.L., Biggerstaff M.S., Lindstrom S., Louie J.K., Christ C.M., Bohm S.R., Fonseca V.P., Ritger K.A., Kuhles D.J., Eggers P., Bruce H., Davidson H.A., Lutterloh E., Harris M.L., Burke C., Cocoros N., Finelli L., MacFarlane K.F., Shu B., Olsen S.J. H1N1 2009 influenza virus infection during pregnancy in the USA. Lancet 2009, 374:451-458.
14. Lackenby A., Hungnes O., Dudman S.G., Meijer A., Paget W.J., Hay A.J., Zambon M.C. Emergence of resistance to oseltamivir among influenza A(H1N1) viruses in Europe. Euro Surveill 2008, 13. pii 8026.
15. Le Q.M., Wertheim H.F., Tran N.D., van Doorn H.R., Nguyen T.H., Horby P. A community cluster of oseltamivir-resistant cases of 2009 H1N1 influenza. N. Engl. J. Med. 2010, 362:86-87.
16. Louie J.K., Acosta M., Jamieson D.J., Honein M.A. Severe 2009 H1N1 influenza in pregnant and postpartum women in California. N. Engl. J. Med. 2010, 362:27-35.
17. National Institute of Technology Evaluation (NITE), National Institute of Infectious Diseases (NIID) NITE/NIID Protocol for Sequencing Influenza A (H1N1) SWL Viral Genome Segments version 1.2 2009, http://www.bio.nite.go.jp/ngac/flu_sequence_protocol.pdf (accessed 7.07.10).
18. Saito R., Oshitani H., Masuda H., Suzuki H. Detection of amantadine-resistant influenza A virus strains in nursing homes by PCR-restriction fragment length polymorphism analysis with nasopharyngeal swabs. J. Clin. Microbiol. 2002, 40:84-88.
19. Sheu T.G., Deyde V.M., Okomo-Adhiambo M., Garten R.J., Xu X., Bright R.A., Butler E.N., Wallis T.R., Klimov A.I., Gubareva L.V. Surveillance for neuraminidase inhibitor resistance among human influenza A and B viruses circulating worldwide from 2004 to 2008. Antimicrob. Agents Chemother. 2008, 52:3284-3292.
20. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24:1596-1599.
21. Tisdale M. Monitoring of viral susceptibility: new challenges with the development of influenza NA inhibitors. Rev. Med. Virol. 2000, 10:45-55.
22. van der Vries E., Jonges M., Herfst S., Maaskant J., Van der Linden A., Guldemeester J., Aron G.I., Bestebroer T.M., Koopmans M., Meijer A., Fouchier R.A., Osterhaus A.D., Boucher C.A., Schutten M. Evaluation of a rapid molecular algorithm for detection of pandemic influenza A (H1N1) 2009 virus and screening for a key oseltamivir resistance (H275Y) substitution in neuraminidase. J. Clin. Virol. 2009, 47:34-37.
23. Wetherall N.T., Trivedi T., Zeller J., Hodges-Savola C., McKimm-Breschkin J.L., Zambon M., Hayden F.G. Evaluation of neuraminidase enzyme assays using different substrates to measure susceptibility of influenza virus clinical isolates to neuraminidase inhibitors: report of the neuraminidase inhibitor susceptibility network. J. Clin. Microbiol. 2003, 41:742-750.
24. World Health Organization What is Phase 6?-Updated 11 June 2009 2009, http://www.who.int/csr/disease/swineflu/frequently_asked_questions/levels_pandemic_alert/en/ (accessed 1.04.10).
25. World Health Organization Pandemic (H1N1) 2009-Update 94 2009, http://www.who.int/csr/don/2010_04_01/en/index.html (accessed 1.04.10).
26. World Health Organization Pandemic (H1N1) 2009 Antiviral Drug Resistance-22 December 2009 2009, http://www.who.int/csr/disease/swineflu/frequently_asked_questions/antivirals/resistance/en/index.html (accessed 1.04.10).
27. World Health Organization Influenza A(H1N1) NA-H274 Detailed Pyrosequencing Protocol for Antiviral Susceptibility Testing: 13 May 2009 2009, http://www.who.int/csr/resources/publications/swineflu/NA_DetailedPyrosequencing_20090513.pdf (accessed 1.04.10).
28. World Health Organization Antiviral Drugs for Pandemic (H1N1) 2009 Definitions and Use-22 December 2009 2009, http://www.who.int/csr/disease/swineflu/frequently_asked_questions/antivirals/definitions_use/en/index.html (accessed 1.04.10).
29. World Health Organization Pandemic (H1N1)2009-Update 94. Weekly Virological Surveillance Update 2010, http://www.who.int/csr/disease/swineflu/laboratory1_04_2010/en/index.html (accessed 1.04.10).
30. World Health Organization Weekly Update on Oseltamivir Resistance to Pandemic Influenza A (H1N1) 2009 Viruses 2010, http://www.who.int/csr/disease/swineflu/Oseltamivirresistant20100312.pdf (accessed 1.04.10).