IC-Tagging and Protein Relocation to ARV muNS Inclusions: A Method to Study Protein-Protein Interactions in the Cytoplasm or Nucleus of Living Cells

PLoS ONE

Volumn 5, Issue 11, 2010, Pages

  Brandariz Nuñez, Alberto ^a   Menaya Vargas, Rebeca ^a   Benavente, Javier ^a   Martinez Costas, Jose ^a  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

LIGAND; PROTEIN MUNS; PROTEIN P53; UNCLASSIFIED DRUG; VIRUS PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PEPTIDE FRAGMENT;

ANIMAL CELL; ARTICLE; AVIAN ORTHOREOVIRUS; CELL INCLUSION; CELL NUCLEUS; CHIMERA; CONTROLLED STUDY; CYTOPLASM; FIBROBLAST CULTURE; GENETIC TRANSFECTION; NONHUMAN; PROTEIN PROTEIN INTERACTION; ANIMAL; CELL CULTURE; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; CHICK EMBRYO; CYTOLOGY; FIBROBLAST; FLUORESCENCE MICROSCOPY; GENETICS; METABOLISM; METHODOLOGY; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN TRANSPORT; VIRUS INCLUSION;

AVES; AVIAN ORTHOREOVIRUS; EUKARYOTA; PRIMATES;

ANIMALS; CELL NUCLEUS; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; CHICK EMBRYO; COS CELLS; CYTOPLASM; FIBROBLASTS; GREEN FLUORESCENT PROTEINS; INCLUSION BODIES, VIRAL; MICROSCOPY, FLUORESCENCE; ORTHOREOVIRUS, AVIAN; PEPTIDE FRAGMENTS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; TUMOR SUPPRESSOR PROTEIN P53; VIRAL NONSTRUCTURAL PROTEINS;

EID: 78249289421     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0013785     Document Type: Article

References (25)

1. Novoa RR, Calderita G, Arranz R, Fontana J, Granzow H, et al. (2005) Virus factories: associations of cell organelles for viral replication and morphogenesis. Biol Cell 97: 147-172.
2. Netherton C, Moffat K, Brooks E, Wileman T (2007) A guide to viral inclusions, membrane rearrangements, factories, and viroplasm produced during virus replication. Adv Virus Res 70: 101-182.
3. Attoui H, Billoir F, Biagini P, de Micco P, de Lamballerie X (2000) Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. J Gen Virol 81: 1507-1515.
4. Mertens P (2004) The dsRNA viruses. Virus Res 101: 3-13.
5. Jones RC (2000) Avian reovirus infections. Rev Sci Tech 19: 614-625.
6. van der Heide L (2000) The history of avian reovirus. Avian Dis 44: 638-641.
7. Zhang X, Tang J, Walker SB, O'Hara D, Nibert ML, et al. (2005) Structure of avian Orthoreovirus virion by electron cryo-microscopy and image reconstruction. Virology 343: 25-35.
8. Benavente J, Martinez-Costas J (2006) Early steps in avian reovirus morphogenesis. Curr Top Microbiol Immunol 309: 67-85.
9. Benavente J, Martinez-Costas J (2007) Avian reovirus: structure and biology. Virus Res 123: 105-119.
10. Costas-Iglesias C, Martinez-Costas J, Bodelon G, Benavente J (2005) The second open reading frame of the avian reovirus S1 gene encodes a transcriptiondependent and CRM1-independent nucleocytoplasmic shuttling protein. J Virol 79: 2141-2150.
11. Vazquez-Iglesias L, Lostale-Seijo I, Martinez-Costas J, Benavente J (2009) Avian reovirus sigmaA localizes to the nucleolus and enters the nucleus by a nonclassical energy- and carrier-independent pathway. J Virol 83: 10163-10175.
12. Touris-Otero F, Martinez-Costas J, Vakharia VN, Benavente J (2004) Avian reovirus non-structural protein microNS forms viroplasm-like inclusions and recruits protein sigmaNS to these structures. Virology 319: 94-106.
13. Touris-Otero F, Cortez-San Martin M, Martinez-Costas J, Benavente J (2004) Avian reovirus morphogenesis occurs within viral factories and begins with the selective recruitment of sigmaNS and lambdaA to microNS inclusions. J Mol Biol 341: 361-374.
14. Brandariz-Nuñez A, Menaya-Vargas R, Benavente J, Martinez-Costas J (2010) Avian reovirus muNS protein forms homo-oligomeric inclusions in a microtubule-independent fashion, which involves specific regions of its Cterminal domain. J Virol 84: 4289-4301.
15. Ali SH, DeCaprio JA (2001) Cellular transformation by SV40 large T antigen: interaction with host proteins. Semin Cancer Biol 11: 15-23.
16. Gluzman Y (1981) SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182.
17. Kalderon D, Roberts BL, Richardson WD, Smith AE (1984) A short amino acid sequence able to specify nuclear location. Cell 39: 499-509.
18. Fujimuro M, Sawada H, Yokosawa H (1994) Production and characterisation of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins. FEBS Lett 349: 173-180.
19. Guan H, Kiss-Toth E (2008) Advanced technologies for studies on protein interactomes. Adv Biochem Eng Biotechnol 110: 1-24.
20. Lievens S, Lemmens I, Tavernier T (2009) Mammaliam two-hybrids come of age. Trends Biochem Sci 34: 579-588.
21. Miller CL, Arnold MM, Broering TJ, Eichwald C, Kim J, et al. (2007) Virusderived platforms for visualizing protein associations inside cells. Mol Cell Proteomics 6: 1027-1038.
22. Schmitz AM, Morrison MF, Agunwamba AO, Nibert ML, Lesser CF (2009) Protein interaction platforms: visualization of interacting proteins in yeast. Nature Methods 6: 500-502.
23. Martinez-Costas J, Varela R, Benavente J (1995) Endogenous enzymatic activities of the avian reovirus S1133: identification of the viral capping enzyme. Virology 206: 1017-1026.
24. Zalvide J, DeCaprio JA (1995) Role of pRb-related proteins in simian virus 40 large-T-antigen-mediated transformation. Mol Cell Biol 10: 5800-5810.
25. Brandariz-Nuñez J, Menaya-Vargas R, Benavente J, Martinez-Costas J (2010) A versatile molecular tagging method that targets proteins to avian reovirus protein muNS inclusions. Use in Protein Immobilization and Purification. PLoS ONE. In press.