Characterization of pseudomonas aeruginosa in burn patients using pcr restriction fragment length polymorphism and random amplified polymorphic dna analysis

Iranian Journal of Medical Sciences

Volumn 35, Issue 3, 2010, Pages 236-241

  Salimi, Hassan ^a   Owlia, Parviz ^b   Yakhchali, Bagher ^a   Lari, Abdolaziz Rastegar ^c  

^a NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

^b SHAHED UNIVERSITY   (Iran)

^c IRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Burn; Pseudomonas aeruginosa; Rapd; Rflp

Indexed keywords

AGAROSE; RESTRICTION ENDONUCLEASE; RNA 16S;

ADOLESCENT; AGAR GEL ELECTROPHORESIS; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL STRAIN; BACTERIAL TRANSMISSION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BURN; CONTROLLED STUDY; FEMALE; GENETIC VARIABILITY; HUMAN; INTERMETHOD COMPARISON; IRAN; MALE; MOLECULAR EPIDEMIOLOGY; MOLECULAR TYPING; NONHUMAN; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; RANDOM AMPLIFIED POLYMORPHIC DNA; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

EID: 78249276185     PISSN: 02530716     EISSN: 17353688     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Church D, Elsayed S, Reid O, et al. Burn wound infections. Clin Microbiol Rev 2006; 19: 403-34.
2. Polavarapu N, Ogilvie MP, Panthaki ZJ. Microbiology of burn wound infections. J Craniofac Surg 2008; 19: 899-902.
3. Rastegar Lari A, Bahrami Honar H, Alaghehbandan R. Pseudomonas infections in Tohid Burn Center, Iran. Burns 1998; 24: 637-41.
4. Ahangarzadeh-Rezaee M, Behzadiyan Nejad Q, Owlia P, et al. In vitro activity of imipenem and ceftazidime against mucoid and non-mucoid strains of Pseudomonas aeruginosa isolated from patients in Iran. Arch Iran Med 2002; 5: 251-4.
5. Behzadiyan-Nejad Q, Souri E, Owlia P. In vitro effects of sub-inhibitory concentrations of gentamicin on P. aeruginosa alginate production. Pharm Pharmacol Commun 1998; 4: 489-91.
6. Rezaee MA, Behzadiyan-Nejad Q, Pirayeh SN, et al. Higher aminoglycoside resistance in mucoid Pseudomonas aeruginosa than in non-mucoid strains. Arch Iran Med 2002; 5: 108-10.
7. Patrick R, Murray RP, Baron EJ, Jorgensen J, Landry ML: Manual of Clinical Microbiology. 9th ed. Washington, DC, ASM, 2007.
8. Gürtler V, Mayall BC. Genomic approaches to typing, taxonomy and evolution of bacterial isolates. Int J Syst Evol Microbiol 2001; 51: 3-16.
9. comparative study of different PCR-based DNA fingerprinting techniques for typing of the Acinetobacter calcoaceticus-A. baumannii complex. J Med Microbiol 1996; 44:482-9.
10. Ranjbar R, Owlia P, Saderi H, et al. Isolation of clinical strains of Pseudomonas aeruginosa Harboring Different Plasmids. Pak J Biol Sci 2007; 10: 3020-2.
11. Sambrook J. Molecular cloning: A laboratory manual. 3rd ed. New York, NY, Cold Spring Harbor Laboratory, 2001.
12. Rosen S, Skaletsky HJ. Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S, editors. Bioinformatics Methods and Protocols: Methods in Molecular Biology.Totowa, NJ, Humana Press; 2000. p. 365-86.
13. Puente Redondo VA, Navas Méndez J, García del Blanco N, et al. Typing of Haemophilus parasuis strains by PCR RFLP analysis of the tbpA gene. Vet Microbiol 2003; 92: 253-62.
14. Flemming L, Rawlings D, Chenia H. Phenotypic and molecular characterisation of fish-borne Flavobacterium johnsoniae-like isolates from aquaculture systems in South Africa. Res Microbiol 2007; 158: 18-30.
15. Mahenthiralingam E, Campbell ME, Foster J, et al. Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol 1996; 34: 1129-35.
16. Renders N, Römling Y, Verbrugh H, et al. Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed-field gel electrophoresis of DNA macrorestriction fragments. J Clin Microbiol 1996; 34: 3190-5.
17. Eftekhar F, Khodadad A, Henry D, Speert D.P. Isolation and genetic fingerprinting of Pseudomonas aeruginosa from Iranian patients with cystic fibrosis using RAPD PCR. Iranian Journal of Biotechnology 2003; 1: 95-100.
18. Bertrand X, Thouverez M, Talon D, et al. Endemicity, molecular diversity and colonisation routes of Pseudomonas aeruginosa in intensive care units. Intensive Care Med 2001; 27: 1263-8.