Degenerate primers for PCR amplification and sequencing of the avian influenza A neuraminidase gene

Journal of Virological Methods

Volumn 170, Issue 1-2, 2010, Pages 94-98

  Orozovic, Goran ^a   Latorre Margalef, Neus ^a   Wahlgren, John ^b,c   Muradrasoli, Shaman ^d   Olsen, Björn ^e  

^a LINNAEUS UNIVERSITY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c SWEDISH INSTITUTE FOR INFECTIOUS DISEASE CONTROL   (Sweden)

^d SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^e UPPSALA UNIVERSITY   (Sweden)

Author keywords

Degenerate primers; Influenza A virus; Neuraminidase; PCR; Sequencing

Indexed keywords

COMPLEMENTARY DNA; PRIMER DNA; SIALIDASE;

ARTICLE; AVIAN INFLUENZA; AVIAN INFLUENZA VIRUS; CONTROLLED STUDY; GENE AMPLIFICATION; GENE SEQUENCE; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; VIRUS DETECTION; VIRUS IDENTIFICATION; VIRUS ISOLATION; VIRUS STRAIN;

ANIMALS; BASE SEQUENCE; DNA PRIMERS; DNA, COMPLEMENTARY; DUCKS; INFLUENZA A VIRUS; INFLUENZA IN BIRDS; NEURAMINIDASE; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, VIRAL; SEQUENCE ANALYSIS, RNA;

AVES; INFLUENZA A VIRUS;

EID: 78249257293     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.09.006     Document Type: Article

References (14)

1. Alvarez A, Brunck M., Boyd V., Lai R., Virtue E., Chen W., Bletchly C., Heine H., Barnard R. A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus. Virology Journal 2008, 5:77-177.
2. Chang H.K., Park J.H., Song M.S., Oh T.K., Kim S.Y., Kim C.J., Kim H., Sung M.H., Han H.S., Hahn Y.S., Choi Y.K. Development of multiplex RT-PCR assays for rapid detection and subtyping of influenza type A viruses from clinical specimens. Journal of Microbiology and Biotechnology 2008, 18:1164-1169.
3. Fouchier R.A.M., Munster V., Wallensten A. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from Black-Headed Gulls. Journal of Virology 2005, 79:2814-2823.
4. Hoffmann E., Krauss S., Perez D., Webby R., Webster R.G. Eight-plasmid system for rapid generation of influenza virus vaccines. Vaccine 2002, 20:3165-3170.
5. Hoffmann E., Stech J., Guan Y., Webster R.G., Perez D.R. Universal primer set for the full-length amplification of all influenza A viruses. Archives of Virology 2001, 146:2275-2289.
6. Jindal N., Chander Y., de Abin M., Sreevatsan S., Stallknecht D., Halvorson D.A., Goyal S.M. Amplification of four genes of influenza A viruses using a degenerate primer set in a one step RT-PCR method. Journal of Virological Methods 2009, 160:163-166.
7. Karlsson M., Wallensten A., Lundkvist A., Olsen B., Brytting M. A real-time PCR assay for the monitoring of influenza a virus in wild birds. Journal of Virological Methods 2007, 144:27-31.
8. Kreibich A., Stech J., Mettenleiter T.C., Stech O. Simultaneous one-tube full-length amplification of the NA, NP, M, and NS genes of influenza A viruses for reverse genetics. Journal of Virological Methods 2009, 159:308-310.
9. Latorre-Margalef N, Gunnarsson G., Munster V.J., Fouchier R.A.M., Osterhaus A., Elmberg J., Olsen B., Wallensten A., Haemig P.D., Fransson T., Brudin L., Waldenstrom J. Effects of influenza A virus infection on migrating mallard ducks. Proceedings of the Royal Society B-Biological Sciences 2009, 276:1029-1036.
10. Lee C.W., Saif Y.A. Avian influenza virus. Comparative Immunology Microbiology and Infectious Diseases 2009, 32:301-310.
11. Olsen B., Munster V.J., Wallensten A., Waldenstrom J., Osterhaus A., Fouchier R.A.M. Global patterns of influenza A virus in wild birds. Science 2006, 312:384-388.
12. Qiu B.F., Liu W.J., Peng D.X., Hu S.L., Tang Y.H., Liu X.F. A reverse transcription-PCR for subtyping of the neuraminidase of avian influenza viruses. Journal of Virological Methods 2009, 155:193-198.
13. Suarez D.L., Das A., Ellis E. Review of rapid molecular diagnostic tools for avian influenza virus. 6th International Symposium on Avian Influenza 2006, 201-208.
14. Wallensten A., Munster V.J., Latorre-Margalef N., Brytting M., Elmberg J., Fouchier R.A.M., Fransson T., Haemig P.D., Karlsson M., Lundkvist A., Osterhaus A., Stervander M., Waldenstrom J., Olsen B. Surveillance of influenza A virus in migratory waterfowl in northern Europe. Emerging Infectious Diseases 2007, 13:404-411.