Simultaneous one-tube full-length amplification of the NA, NP, M, and NS genes of influenza A viruses for reverse genetics

Journal of Virological Methods

Volumn 159, Issue 2, 2009, Pages 308-310

  Kreibich, Anne ^a   Stech, Jürgen ^a   Mettenleiter, Thomas C ^a   Stech, Olga ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

Influenza A virus; Neuraminidase; PCR; Reverse genetics; Universal cloning

Indexed keywords

PRIMER DNA; SIALIDASE;

AMPLICON; ARTICLE; DNA FLANKING REGION; GENE AMPLIFICATION; GENOME SIZE; INFLUENZA VIRUS A; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIRAL GENETICS; VIRUS GENE; VIRUS GENOME;

DNA PRIMERS; INFLUENZA A VIRUS; NEURAMINIDASE; POLYMERASE CHAIN REACTION; RNA-BINDING PROTEINS; VIRAL CORE PROTEINS; VIRAL MATRIX PROTEINS; VIRAL NONSTRUCTURAL PROTEINS; VIRAL PROTEINS;

INFLUENZA A VIRUS;

EID: 67349203996     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.04.020     Document Type: Article

References (11)

1. Adeyefa C.A., Quayle K., and McCauley J.W. A rapid method for the analysis of influenza virus genes: application to the reassortment of equine influenza virus genes. Virus Res 32 (1994) 391-399
2. de Wit E., Spronken M.I., Vervaet G., Rimmelzwaan G.F., Osterhaus A.D., and Fouchier R.A. A reverse-genetics system for Influenza A virus using T7 RNA polymerase. J Gen Virol 88 (2007) 1281-1287
3. Fodor E., Devenish L., Engelhardt O.G., Palese P., Brownlee G.G., and Garcia-Sastre A. Rescue of influenza A virus from recombinant DNA. J Virol 73 (1999) 9679-9682
4. Gabriel G., Dauber B., Wolff T., Planz O., Klenk H.D., and Stech J. The viral polymerase mediates adaptation of an avian influenza virus to a mammalian host. Proc Natl Acad Sci U S A 102 (2005) 18590-18595
5. Hoffmann E., Neumann G., Kawaoka Y., Hobom G., and Webster R.G. A DNA transfection system for generation of influenza A virus from eight plasmids. Proc Natl Acad Sci U S A 97 (2000) 6108-6113
6. Hoffmann E., Stech J., Guan Y., Webster R.G., and Perez D.R. Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol 146 (2001) 2275-2289
7. Massin P., Rodrigues P., Marasescu M., van der Werf S., and Naffakh N. Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza A viruses in avian cells. J Virol 79 (2005) 13811-13816
8. Nayak D.P. Defective interfering influenza viruses. Annu Rev Microbiol 34 (1980) 619-644
9. Neumann G., Watanabe T., Ito H., Watanabe S., Goto H., Gao P., Hughes M., Perez D.R., Donis R., Hoffmann E., Hobom G., and Kawaoka Y. Generation of influenza A viruses entirely from cloned cDNAs. Proc Natl Acad Sci U S A 96 (1999) 9345-9350
10. Skehel J.J., and Hay A.J. Nucleotide sequences at the 5' termini of influenza virus RNAs and their transcripts. Nucleic Acids Res 5 (1978) 1207-1219
11. Stech J., Stech O., Herwig A., Altmeppen H., Hundt J., Gohrbandt S., Kreibich A., Weber S., Klenk H.D., and Mettenleiter T.C. Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification. Nucleic Acids Res 36 (2008) e139