Efficiency of the new sub-2μm core-shell (Kinetex™) column in practice, applied for small and large molecule separation

Journal of Pharmaceutical and Biomedical Analysis

Volumn 54, Issue 3, 2011, Pages 482-490

  Fekete, Szabolcs ^a   Ganzler, Katalin ^a   Fekete, Jeno ^b  

^a GEDEON RICHTER LTD   (Hungary)

^b BUDAPEST UNIVERSITY OF TECHNOLOGY AND ECONOMICS   (Hungary)

Author keywords

Column efficiency; Core shell; Kinetex; Kinetic plots; Sub 2 m particles

Indexed keywords

ESTRADIOL; POLYPEPTIDE;

ARTICLE; CALCULATION; COLUMN CHROMATOGRAPHY; FLOW RATE; KINETICS; MOLECULAR SIZE; MOLECULAR WEIGHT; PERMEABILITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; SEPARATION TECHNIQUE;

CHROMATOGRAPHY, LIQUID; IVERMECTIN; MOLECULAR WEIGHT; PARTICLE SIZE; PEPTIDES; PERMEABILITY; POROSITY; PROTEINS; SILICON DIOXIDE; TEMPERATURE;

EID: 78149495855     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jpba.2010.09.021     Document Type: Article

References (50)

1. Swartz M.E., Murphy B., Sievers D. UPLC: expanding the limits of HPLC. GIT Lab. J. 2004, 8:43-45.
2. Swartz M.E. UPLC: an introduction and review. J. Liq. Chromatogr. 2005, 28:1253-1263.
3. Phillips D.J., Capparella M., Neue U.D., El Fallah Z. A new small particle packing for faster analysis with high resolution. J. Pharm. Biomed. Anal. 1997, 15:1389-1395.
4. Gerber F., Krummen M., Potgeter H., Roth A., Siffrin C., Spoendlin C. Practical aspects of fast reversed-phase high-performance liquid chromatography using 3μm particle packed columns and monolithic columns in pharmaceutical development and production working under current good manufacturing practice. J. Chromatogr. A 2004, 1036:127-133.
5. Knox J.H., Saleem M. Kinetic conditions for optimum speed and resolution in column chromatography. J. Chromatogr. Sci. 1969, 7:614-622.
6. MacNair J.E., Lewis K.C., Jorgenson J.W. Ultrahigh-pressure reversed-phase liquid chromatography in packed capillary columns. Anal. Chem. 1997, 69:983-989.
7. MacNair J.E., Patel K.D., Jorgenson J.W. Ultrahigh-pressure reversed-phase capillary chromatography. Anal. Chem. 1999, 71:700-708.
8. Wu N., Lippert J.A., Lee M.L. Practical aspects of ultrahigh pressure capillary liquid chromatography. J. Chromatogr. A 2001, 911:1-12.
9. Blackwell J.A., Carr P.W. A chromatographic study of the Lewis acid-base chemistry of zirconia surfaces. J. Liq. Chromatogr. 1991, 14:2875-2889.
10. Zhu C., Goodall D.M., Wren S.A.C. Elevated temperature HPLC: principles and applications to small molecules and biomolecules. LCGC N. Am. 2005, 23:1-9.
11. Claessens H.A., van Straten M.A. Reduction of Analysis Times in HPLC at Elevated Column Temperatures 2004, Eindhoven University of Technology.
12. Jackson P.T., Carr P.W. Improving reversed-phase liquid chromatography. ChemTech 1988, 28:29-37.
13. Guillarme D., Heinisch S. Detection modes with high temperature liquid chromatography - a review. Sep. Purif. Rev. 2005, 34:181-216.
14. Hjertén S., Liao J.L., Zhang R. High-performance chromatofocusing of proteins on agarose columns. II. Deformed non-porous 12-15μm beads. J. Chromatogr. 1989, 473:273-275.
15. Wang Q.C., Hosoya K., Svec F., Frechet J.M. Polymeric porogens used in the preparation of novel monodispersed macroporous polymeric separation media for high-performance liquid chromatography. J. Anal. Chem. 1992, 64:1232-1238.
16. Gusev I., Huang X., Horvath C. Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography. J. Chromatogr. A 1999, 885:273-290.
17. Minakuchi H., Nagayama H., Soga N., Ishizuka N., Tanaka N. Effect of domain size on the performance of octadecylsilylated continuous porous silica columns in reversed-phase liquid chromatography. J. Chromatogr. A 1998, 797:121-131.
18. Oberacher H., Premstaller A., Huber C.G. Characterization of some physical and chromatographic properties of monolithic poly(styrene-co-divinylbenzene) columns. J. Chromatogr. A 2004, 1030:201-208.
19. Ikegami T., Dicks E., Kobayashi H., Morisaka H., Tokuda D., Cabrera K., Tanaka N. How to utilize the true performance of monolithic silica columns. J. Sep. Sci. 2004, 27:1292-1302.
20. Horvath C., Preiss B.A., Lipsky S.R. Fast liquid chromatography: an investigation of operating parameters and the separation of nucleotides on pellicular ion exchangers. Anal. Chem. 1967, 39:1422-1428.
21. Horvath C., Lipsky S.R.J. Rapid analysis of ribonucleosides and bases at the picomole level using pellicular cation exchange resin in narrow bore columns. Anal. Chem. 1969, 41:1227-1234.
22. Kirkland J.J. Controlled surface porosity supports for high speed gas and liquid chromatography. Anal. Chem. 1969, 41:218-220.
23. Kirkland J.J., Truszkowski F.A., Dilks C.H., Engel G.S. Superficially porous silica microspheres for fast high-performance liquid chromatography of macromolecules. J. Chromatogr. A 2000, 890:3-13.
24. Gritti F., Guiochon G. Comparative study of the performance of columns packed with several new fine silica particles: would the external roughness of the particles affect column properties?. J. Chromatogr. A 2007, 1166:30-46.
25. Fekete S., Fekete J., Ganzler K. Shell and small particles; evaluation of new column technology. J. Pharm. Biomed. Anal. 2009, 49:64-71.
26. 18 particles in gradient elution chromatography. J. Chromatogr. A 2010, 1217:1604-1615.
27. 18. J. Chromatogr. A 2010, 1217:1589-1603.
28. Gritti F., Leonardis I., Abia J., Guiochon G. Physical properties and structure of fine core-shell particles used as packing materials for chromatography: relationships between particle characteristics and column performance. J. Chromatogr. A 2010, 1217:3819-3843.
29. Gritti F., Sanchez C.A., Farkas T., Goiochon G. Achieving the full performance of highly efficient columns by optimizing conventional benchmark high-performance liquid chromatography instruments. J. Chromatogr. A 2010, 1217:3000-3012.
30. Oláh E., Fekete S., Fekete J., Ganzler K. Comparative study of new shell-type, sub-2 μm fully porous and monolith stationary phases, focusing on mass-transfer resistance. J. Chromatogr. A 2010, 1217:3642-3653.
31. Poppe H. Some reflections on speed and efficiency of modern chromatographic methods. J. Chromatogr. A 1997, 778:3-21.
32. Desmet G., Cabooter D., Gzil P., Verelst H., Mangelings D., Heyden Y.V., Clicq D. Future of high pressure liquid chromatography: do we need porosity or do we need pressure?. J. Chromatogr. A 2006, 1130:158-166.
33. van Deemter J.J., Zuiderweg F.J., Klinkenberg A. Longitudinal diffusion resistance to mass transfer as causes of nonideality in chromatography. Chem. Eng. Sci. 1956, 5:271-289.
34. Giddings J.C. Dynamics of Chromatography Part 1 1965, Marcel Dekker, New York.
35. Giddings J.C. Comparison of theoretical limit of separating speed in gas and liquid chromatography. Anal. Chem. 1965, 37:60-63.
36. Desmet G., Clicq D., Gzil P. Geometry-independent plate height representation methods for the direct comparison of the kinetic performance of LC supports with a different size or morphology. Anal. Chem. 2005, 77:4058-4070.
37. Chen H., Horváth C. High-speed high-performance liquid chromatography of peptides and proteins. J. Chromatogr. A 1995, 705:3-20.
38. Wilke C.R., Chang P. Correlation of diffusion coefficients in dilute solutions. AIChE J. 1955, 264-270.
39. Poling B., Prausnitz J., O'Connel J. The Properties of Gases and Liquids 2001, McGraw-Hill, New York, NY. 5th ed.
40. Gritti F., Fellinger A., Guiochon G. Influence of the errors made in the measurement of the extra-column volume on the accuracies of estimates of the column efficiency and the mass transfer kinetics parameters. J. Chromatogr. A 2006, 1136:57-72.
41. Taylor G. Dispersion of soluble matter in solvent flowing slowly through a tube. Proc. R. Soc. 1953, A186:317-319.
42. Felinger A. Data Analysis, Signal Processing in Chromatography 1998, Elsevier, Amsterdam.
43. Fountain K.J., Neue U.D., Grumbach E.S., Diehl D.M. Effects of extra-column band spreading, liquid chromatography system operating pressure, and column temperature on the performance of sub-2-μm porous particles. J. Chromatogr. A 2009, 1216:5979-5988.
44. Jerkovich A.D., LoBrutto R., Vivilecchia R.V. The use of acquity UPLC™ in pharmaceutical development. LC-GC NA 2005, 15-21.
45. Knox J.H. Band dispersion in chromatography-a universal expression for the contribution from the mobile zone. J. Chromatogr. A 2002, 960:7-18.
46. Kaczmarski K., Guiochon G. Modeling of chromatographic separation using pellicular adsorbent. Anal. Chem. 2007, 79:4648-4656.
47. Bristow P.A., Knox J.H. Standardization of test conditions for high performance liquid chromatography columns. Chromatographia 1977, 10:279-289.
48. Stout R.W., DeStefano J.J., Snyder L.R. High-performance liquid chromatographic column efficiency as a function of particle composition and geometry and capacity factor. J. Chromatogr. 1983, 282:263-272.
49. Knox J.H., Scott H.P. B and C terms in the Van Deemter equation for liquid chromatography. J. Chromatogr. 1983, 282:297-313.
50. Brunauer S., Emmet P., Teller E. Adsorption of gases in multimolecular layers. J. Am. Chem. Soc. 1938, 60:309-319.