Murine rVEGF164b, an inhibitory VEGF reduces VEGF-A-dependent endothelial proliferation and barrier dysfunction

Microcirculation

Volumn 17, Issue 7, 2010, Pages 536-547

  Cromer, Walter ^a   Jennings, Merilyn H ^b   Odaka, Yoshinubo ^a   Mathis, J Michael ^a   Alexander, J Steven ^b  

^a Department of Cellular Biology and Anatomy   (United States)

^b LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

Barrier; Endothelial; Inhibitory; Migration; Proliferation; VEGF A; VEGF164b

Indexed keywords

ACTIN; F ACTIN; PROTEIN ZO1; RECOMBINANT VASCULOTROPIN 164B; UNCLASSIFIED DRUG; VASCULOTROPIN A; VASCULOTROPIN INHIBITOR;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL PROLIFERATION; CYTOSKELETON; DRUG EFFECT; ENDOTHELIUM CELL; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ACTINS; ADENOVIRIDAE; AMINO ACID SEQUENCE; ANIMALS; CAPILLARY PERMEABILITY; CELL MOVEMENT; CELL PROLIFERATION; CELLS, CULTURED; COCULTURE TECHNIQUES; ELECTRIC IMPEDANCE; ENDOTHELIAL CELLS; GENETIC VECTORS; HUMANS; MEMBRANE PROTEINS; MICE; PHOSPHOPROTEINS; RECOMBINANT PROTEINS; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

MURINAE;

EID: 78149251195     PISSN: 10739688     EISSN: 15498719     Source Type: Journal    
DOI: 10.1111/j.1549-8719.2010.00047.x     Document Type: Article

References (30)

1. Albuquerque RJ, Hayashi T, Cho WG, Kleinman ME, Dridi S, Takeda A, Baffi JZ, Yamada K, Kaneko H, Green MG and others. Alternatively spliced vascular endothelial growth factor receptor-2 is an essential endogenous inhibitor of lymphatic vessel growth. Nat Med 15: 1023-1030, 2009.
2. Ambati BK, Patterson E, Jani P, Jenkins C, Higgins E, Singh N, Suthar T, Vira N, Smith K, Caldwell R. Soluble vascular endothelial growth factor receptor-1 contributes to the corneal antiangiogenic barrier. Br J Ophthalmol 91: 505-508, 2007.
3. Ando T, Jordan P, Joh T, Wang Y, Jennings MH, Houghton J, Alexander JS. Isolation and characterization of a novel mouse lymphatic endothelial cell line: SV-LEC. Lymphat Res Biol 3: 105- 115, 2005.
4. Appleton I, Brown NJ, Willis D, Colville-Nash PR, Alam C, Brown JR, Willoughby DA. The role of vascular endothelial growth factor in a murine chronic granulomatous tissue air pouch model of angiogenesis. J Pathol 180: 90-94, 1996.
5. Barkefors I, Le Jan S, Jakobsson L, Hejll E, Carlson G, Johansson H, Jarvius J, Park JW, Li Jeon N, Kreuger J. Endothelial cell migration in stable gradients of vascular endothelial growth factor A and fibroblast growth factor 2: effects on chemotaxis and chemokinesis. J Biol Chem 283: 13905-13912, 2008.
6. Bates DO, Cui TG, Doughty JM, Winkler M, Sugiono M, Shields JD, Peat D, Gillatt D, Harper SJ. VEGF165b, an inhibitory splice variant of vascular endothelial growth factor, is down-regulated in renal cell carcinoma. Cancer Res 62: 4123-4131, 2002.
7. Chen YH, Wu HL, Chen CK, Huang YH, Yang BC, Wu LW. Angiostatin antagonizes the action of VEGF-A in human endothelial cells via two distinct pathways. Biochem Biophys Res Commun 310: 804-810, 2003.
8. Conn G, Soderman DD, Schaeffer MT, Wile M, Hatcher VB, Thomas KA. Purification of a glycoprotein vascular endothelial cell mitogen from a rat glioma-derived cell line. Proc Natl Acad Sci USA 87: 1323-1327, 1990.
9. Glass CA, Harper SJ, Bates DO. The anti-angiogenic VEGF isoform VEGF165b transiently increases hydraulic conductivity, probably through VEGF receptor 1 in vivo. J Physiol 572: 243-257, 2006.
10. Iruela-Arispe ML, Porter P, Bornstein P, Sage EH. Thrombospondin-1, an inhibitor of angiogenesis, is regulated by progesterone in the human endometrium. J Clin Invest 97: 403-412, 1996.
11. Kevil CG, De Benedetti A, Payne DK, Coe LL, Laroux FS, Alexander JS. Translational regulation of vascular permeability factor by eukaryotic initiation factor 4E: implications for tumor angiogenesis. Int J Cancer 65: 785-790, 1996.
12. Li CX, Poznansky MJ. Characterization of the ZO-1 protein in endothelial and other cell lines. J Cell Sci 97: 231-237, 1990.
13. Magnussen ALCA, Harper S, Floege J, Bates D. VEGF165b is more potent at inhibiting endothelial cell migration than Pegabtanib and is cytoprotective for retinal pigmented epithelial cells. FASEB J 22: 746, 2008.
14. Muhlhauser J, Merrill MJ, Pili R, Maeda H, Bacic M, Bewig B, Passaniti A, Edwards NA, Crystal RG, Capogrossi MC. VEGF165 expressed by a replication-deficient recombinant adenovirus vector induces angiogenesis in vivo. Circ Res 77: 1077-1086, 1995.
15. Nowak DG, Woolard J, Amin EM, Konopatskaya O, Saleem MA, Churchill AJ, Ladomery MR, Harper SJ, Bates DO. Expression of proand anti-angiogenic isoforms of VEGF is differentially regulated by splicing and growth factors. J Cell Sci 121: 3487-3495, 2008.
16. Obinata M. Conditionally immortalized cell lines with differentiated functions established from temperature-sensitive T-antigen transgenic mice. Genes Cells 2: 235-244, 1997.
17. Olsson AK, Dimberg A, Kreuger J, Claesson-Welsh L. VEGF receptor signalling-in control of vascular function. Nat Rev Mol Cell Biol 7: 359-371, 2006.
18. Rennel E, Waine E, Guan H, Schuler Y, Leenders W, Woolard J, Sugiono M, Gillatt D, Kleinerman E, Bates D and others. The endogenous anti-angiogenic VEGF isoform, VEGF165b inhibits human tumour growth in mice. Br J Cancer 98: 1250-1257, 2008.
19. Robin A, Ten Brick DTC, Jeremy Martin L, Karl Moline V, Jeffery Bergman W, Andrea Cupp S. Vascular endothelial growth factor inhibitory isoform is regulated prior to ovulation. Nebraska Beef Cattle Rep 1: 113-115, 2007.
20. Rousseau S, Houle F, Kotanides H, Witte L, Waltenberger J, Landry J, Huot J. Vascular endothelial growth factor (VEGF)-driven actinbased motility is mediated by VEGFR2 and requires concerted activation of stress-activated protein kinase 2 (SAPK2/p38) and geldanamycin-sensitive phosphorylation of focal adhesion kinase. J Biol Chem 275: 10661-10672, 2000.
21. Scharpfenecker M, Fiedler U, Reiss Y, Augustin HG. The Tie-2 ligand angiopoietin-2 destabilizes quiescent endothelium through an internal autocrine loop mechanism. J Cell Sci 118: 771-780, 2005.
22. Scott A, Mellor H. VEGF receptor trafficking in angiogenesis. Biochem Soc Trans 37: 1184-1188, 2009.
23. Song B, Lu K, Zhang Y, Guo S, Han Y, Ma F, Li H. Angiogenesis in hypertrophic scar of rabbit ears and effect of extracellular protein with metalloprotease and thrombospondin 1 domains on hypertrophic scar. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 22: 70- 74, 2008.
24. Spyridopoulos I, Luedemann C, Chen D, Kearney M, Chen D, Murohara T, Principe N, Isner JM, Losordo DW. Divergence of angiogenic and vascular permeability signaling by VEGF: inhibition of protein kinase C suppresses VEGF-induced angiogenesis, but promotes VEGF-induced, NO-dependent vascular permeability. Arterioscler Thromb Vasc Biol 22: 901-906, 2002.
25. Usui T, Ishida S, Yamashiro K, Kaji Y, Poulaki V, Moore J, Moore T, Amano S, Horikawa Y, Dartt D, Golding M, Shima DT, Adamis AP. VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2. Invest Ophthalmol Vis Sci 45: 368-374, 2004.
26. Van Itallie CM, Fanning AS, Bridges A, Anderson JM. ZO-1 stabilizes the tight junction solute barrier through coupling to the perijunctional cytoskeleton. Mol Biol Cell 20: 3930-3940, 2009.
27. Varey AH, Rennel ES, Qiu Y, Bevan HS, Perrin RM, Raffy S, Dixon AR, Paraskeva C, Zaccheo O, Hassan AB and others. VEGF 165 b, an antiangiogenic VEGF-A isoform, binds and inhibits bevacizumab treatment in experimental colorectal carcinoma: balance of proand antiangiogenic VEGF-A isoforms has implications for therapy. Br J Cancer 98: 1366-1379, 2008.
28. Woolard J, Wang WY, Bevan HS, Qiu Y, Morbidelli L, PritchardJones RO, Cui TG, Sugiono M, Waine E, Perrin R; others. VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Res 64: 7822-7835, 2004.
29. Wright GL, Maroulakou IG, Eldridge J, Liby TL, Sridharan V, Tsichlis PN, Muise-Helmericks RC. VEGF stimulation of mitochondrial biogenesis: requirement of AKT3 kinase. FASEB J 22: 3264-3275, 2008.
30. Yang GY, Xu B, Hashimoto T, Huey M, Chaly T Jr, Wen R, Young WL. Induction of focal angiogenesis through adenoviral vector mediated vascular endothelial cell growth factor gene transfer in the mature mouse brain. Angiogenesis 6: 151-158, 2003.