Inhibitory actions of mibefradil on steroidogenesis in mouse Leydig cells: Involvement of Ca 2+ entry via the T-type Ca 2+ channel

Asian Journal of Andrology

Volumn 12, Issue 6, 2010, Pages 807-813

  Lee, Jae Ho ^a   Kim, Jong Uk ^b   Kim, Changhoon ^c   Min, Churl K ^b  

^a BK21 Plus NBM Global Research Center for Regenerative Medicine   (South Korea)

^b Ajou University   (South Korea)

^c EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Leydig cells; mibefradil; StAR; steroidogenesis; T type Ca2+ channel

Indexed keywords

CALCIUM; CALCIUM CHANNEL T TYPE; CHORIONIC GONADOTROPIN; MESSENGER RNA; MIBEFRADIL; STEROIDOGENIC ACUTE REGULATORY PROTEIN; TESTOSTERONE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CALCIUM CURRENT; CONTROLLED STUDY; DOSE RESPONSE; EXTRACELLULAR CALCIUM; HORMONE SYNTHESIS; IN VITRO STUDY; LEYDIG CELL; MALE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PATCH CLAMP; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; RADIOIMMUNOASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEROIDOGENESIS; TESTOSTERONE RELEASE;

ANIMALS; CALCIUM; CALCIUM CHANNEL BLOCKERS; CALCIUM CHANNELS, T-TYPE; CHORIONIC GONADOTROPIN; LEYDIG CELLS; MALE; MIBEFRADIL; MICE; MICE, INBRED ICR; PHOSPHOPROTEINS; STEROIDS;

EID: 78049519068     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1038/aja.2010.51     Document Type: Article

References (35)

1. Dufau ML. Endocrine regulation and communicating functions of the Leydig cell. Ann Rev Physiol 1988;50:483-508.
2. Saez JM. Leydig cells: endocrine, paracrine, and autocrine regulation. Endocr Rev 1994;15:574-626.
3. Chen YC, Nagpal ML, Stocco DM, Lin T. Effects of genistein, resveratrol, and quercetin on steroidogenesis and proliferation of MA-10 mouse Leydig tumor cells. J Endocrinol 2007;192:527-37.
4. Medelson C, Dufau ML, Catt KJ. Gonadotropin binding and stimulation of cAMP and testosterone production in isolated Leydig cells. J Biol Chem 1975;250:8818-23.
5. 2+ by lutropin (LH), luliberin (LHRH) agonist and cyclic AMP. Biochem J 1986;236:45-51.
6. Tomic M, Dufau ML, Catt KJ, Stojilkovic SS. Calcium signalling in single rat Leydig cells. Endocrinology 1995;136:3422-29.
7. Steele GL, Leung PC. Intragonadal signalling mechanisms in the control of steroid hormone production. J Steroid Biochem Mol Biol 1992;41:515-22.
8. Li LH, Wine RN, Miller DS, Reece JM, Smith M, et al. Protection against methoxyacetic-acid-induced spermatocyte apoptosis with calcium channel blockers in cultured rat seminiferous tubules: possible mechanisms. Toxicol Appl Pharmacol 1997;144:105-19.
9. 2+ channels and the acrosome reaction: which channels are present and what do they do? Hum Reprod 1999;14:873-9.
10. Berridge MJ, Lipp P, Bootman MD. The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol 2000;1:11-21.
11. Rommerts FF, Lyng FM, von Ledebur E, Quinlan L, Jones GR, et al. Calcium confusion-is the variability in calcium response by Sertoli cells to specifc hormones meaningful or simply redundant? J Endocrinol 2000;167:1-5.
12. Rossato M, Nogara A, Merico M, Ferlin A, Garolla A, et al. Store-operated calcium influx and stimulation of steroidogenesis in rat Leydig cells: role of Ca (2+)-activated K (+) channels. Endocrinology 2001;142:3865-72.
13. 2+ current. J Biol Chem 2003;278:33730-7.
14. Bergh JJ, Xu Y, Farach-Carson MC. Osteoprotegerin expression and secretion are regulated by calcium influx through the L-type voltage-sensitive calcium channel. Endocrinology 2004;145:426-36.
15. Yamaguchi M. Role of regucalcin in maintaining cell homeostasis and function. Int J Mol Med 2005;15:371-89.
16. Hille B. Calcium channels. In: Hille B, ed. Ionic Channels of Excitable Membranes, 2nd edn. Sunderland, MA: Sinauer; 1992. p83.
17. 2+-sensing receptor. J Physiol 1998;513:399-410.
18. Kawa K. Existence of calcium channels and intercellular coupling in the testosterone-secreting cells of the mouse. J Physiol 1987;393:647-66.
19. 2+ current of mouse spermatogenic cells. Mol Pharmacol 1998;53:1104-11.
20. Costa RR, Varanda WA. Intracellular calcium changes in mice Leydig cells are dependent on calcium entry through T-type calcium channels. J Physiol 2007;585:339-49.
21. Lalevee N, Pluciennik F, Joffre M. Voltage-dependent calcium current with properties of T-type current in Sertoli cells from immature rat testis in primary cultures. Biol Reprod 1997;56:680-7.
22. De Paoli P, Cerbai E, Koidl B, Kirchengast M, Sartiani L, et al. Selectivity of different calcium antagonists on T-and L-type calcium currents in guinea-pig ventricular myocytes. Pharmacol Res 2002;46:491-7.
23. Leuranguer V, Mangoni ME, Nargeot J, Richard S. Inhibition of T-type and L-type calcium channels by mibefradil: physiologic and pharmacologic bases of cardiovascular effects. J Cardiovasc Pharmacol 2001;37:649-61.
24. Lin H, Wang SW, Wang RY, Wang PS. Stimulatory effect of lactate on testosterone production by rat Leydig cells. J Cell Biochem 2001;83:147-54.
25. Ge R, Hardy MP. Protein kinase C increases 11β-hydroxyasteroid dehydrogenase oxidation and inhibits reduction in rat Leydig cells. J Androl 2002;23:135-43.
26. Hamill OP, Marty E, Neher E, Sakman B, Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pfügers Arch 1981;391:85-100.
27. 2+ on the testosterone production of mouse primary Leydig cell culture. J Toxicol Environ Health 1998;55:213-24.
28. Manna PR, Pakarinen P, El-Hefnawy T, Huhtaniemi IT. Functional assessment of the calcium messenger system in cultured mouse Leydig tumor cells: regulation of human chorionic gonadotropin-induced expression of the steroidogenic acute regulatory protein. Endocrinology 1999;140:1739-51.
29. Stocco DM. StAR protein and the regulation of steroid hormone biosynthesis. Annu Rev Physiol 2001;63:193-213.
30. 2+ currents in freshly dispersed smooth muscle cells from the human proximal urethra. J Physiol 2003;550:753-64.
31. Manna PR, Huhtaniemi IT, Stocco DM. Detection of hCG responsive expression of the steroidogenic acute regulatory protein in mouse Leydig cells. Biol Proc Online 2004;6:83-93.
32. Kumar S, Blumberg DL, Canas JA, Maddaiah VT. Human chorionic gonadotropin (hCG) increases cytosolic free calcium in adult rat Leydig cells. Cell Calcium 1994;15:349-55.
33. Wũrthner JU, Kistler M, Kratzmeier M, Mukhopadhyay AK. LH/hCG-receptor is coupled to both adenylate cyclase and protein kinase C signaling pathways in isolated mouse Leydig cells. Endocrine 1995;3:579-84.
34. Pandey AK, Li W, Yin X, Stocco DM, Grammas P, et al. Blocking L-type calcium channels reduced the threshold of cAMP-induced steroidogenic acute regulatory gene expression in MA-10 mouse Leydig cells. J Endocrinol 2010;204:67-74.
35. Dyson MT, Kowalewski MP, Manna PR, Stocco DM. The differential regulation of steroidogenic acute regulatory protein-mediated steroidogenesis by type I and type II PKA in MA-10 cells. Mol Cell Endocrinol 2009;300:94-103.