Expression of inulinase gene in the oleaginous yeast Yarrowia lipolytica and single cell oil production from inulin-containingmaterials

Metabolic Engineering

Volumn 12, Issue 6, 2010, Pages 510-517

  Zhao, Chun Hai ^a,b   Cui, Wei ^a   Liu, Xiao Yan ^a   Chi, Zhen Ming ^a   Madzak, Catherine ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

^b Shandong Binzhou Vocational College   (China)

Author keywords

Exo inulinase; Gene expression; Oleaginous yeast; Single cell oil; Yarrowia lipolytica

Indexed keywords

CELL DRY WEIGHT; CULTIVATION CONDITIONS; HIS-TAG; INULINASE; JERUSALEM ARTICHOKE; JERUSALEM ARTICHOKE TUBERS; KLUYVEROMYCES MARXIANUS; OLEAGINOUS YEASTS; SINGLE CELL OIL; TRANSFORMANT CELLS; YARROWIA LIPOLYTICA; YEAST CELL;

CULTIVATION; CYTOLOGY; FATTY ACIDS; FERMENTATION; GENE ENCODING; GENE EXPRESSION; GROWTH KINETICS; SUGAR (SUCROSE); YEAST;

CELLS;

FATTY ACID; INULIN; INULINASE; OIL;

ARTICLE; CELL GROWTH; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; KLUYVEROMYCES MARXIANUS; LIPID COMPOSITION; MOLECULAR CLONING; PRIORITY JOURNAL; YARROWIA LIPOLYTICA;

BIOENGINEERING; CARBOHYDRATES; CLONING, MOLECULAR; CULTURE MEDIA; DNA, FUNGAL; FATTY ACIDS; FERMENTATION; GENETIC VECTORS; GLYCOSIDE HYDROLASES; HELIANTHUS; INULIN; KLUYVEROMYCES; NITROGEN; OILS; PLANT EXTRACTS; PLANT ROOTS; PLASMIDS; TRANSFORMATION, GENETIC; URACIL; YARROWIA;

HELIANTHUS TUBEROSUS; KLUYVEROMYCES MARXIANUS; YARROWIA LIPOLYTICA;

EID: 78049417511     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2010.09.001     Document Type: Article

References (28)

1. Beopoulos A., Mrozova Z., Thevenieau F., Le Dall M.T., Hapala I., Papanikolaou S., Chardot T., Nicaud J.M. Control of lipid accumulation in the yeast Yarrowia lipolytica. Appl. Environ. Microbiol. 2008, 74:7779-7789.
2. Chi Z.M., Liu J., Zhang W. Trehalose accumulation from soluble starch by Saccharomycopsis fibuligera sdu. Enzyme Microb. Technol. 2001, 38:240-246.
3. Chi Z.M., Chi Z., Zhang T., Liu G.L., Yue L.X. Inulinase expressing microorganisms and applications of inulinases. Appl. Microbiol. Biotechnol. 2009, 82:211-220.
4. Folch J., Lees M., Slane-Stanley J.A. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957, 226:497-509.
5. Gong F., Sheng J., Chi Z.M., Li J. Inulinase production by a marine yeast Pichia guilliermondii and inulin hydrolysis by the crude inulinase. J. Ind. Microbiol. Biotechnol. 2007, 34:179-185.
6. Hassan M., Blanc P.J., Granger L.M., Pareilleux A., Goma G. Influence of nitrogen and iron limitations on lipid production by Cryptococcus curvatus grown in batch and fed-batch culture. Proc. Biochem. 1996, 31:355-361.
7. Helwani Z., Othman M.R., Aziz N., Fernando W.J.N., Kim J. Technologies for production of biodiesel focusing on green catalytic techniques: a review. Fuel Proc. Technol. 2009, 90:1502-1514.
8. Jolivalt C., Madzak C., Brault A., Caminade E., Malosse C., Mougin C. Expression of laccase lllb from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications. Appl. Microbiol. Biotechnol. 2005, 66:450-456.
9. Li Q., Du W., Liu D. Perspectives of microbial oils for biodiesel production. Appl. Microbiol. Biotechnol. 2007, 80:749-756.
10. Li M., Liu G.L., Chi Z., Chi Z.M. Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TJY15a. Biomass Bioenergy 2010, 4:101-107.
11. Liu X.Y., Chi Z., Liu G.L., Wang F., Madzak C., Chi Z.M. Inulin hydrolysis and citric acid production from inulin using the surface-engineered Yarrowia lipolytica displaying inulinase. Metab. Eng. 2010, 12:469-476.
12. Madzak C., Gaillardin C., Beckerich J.M. Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. J. Biotechnol. 2004, 109:63-68.
13. Meng X., Yang J., Xu X., Zhang L., Nie Q., Xian M. Biodiesel production from oleaginous microorganisms. Renew. Energy 2009, 34:1-5.
14. Nicaud J.M., Madzak C., vanden Broek P., Gysler C., Duboc P., Niederberger P., Gaillardin C. Protein expression and secretion in the yeast Yarrowia lipolytica. FEMS Yeast Res. 2002, 2:371-379.
15. Papanikolaou S., Chevalot I., Komaitis M., Marc I., Aggelis G. Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures. Appl. Microbiol. Biotechnol. 2002, 58:308-312.
16. Papanikolaou S., Aggelis G. Selective uptake of fatty acids by the yeast Yarrowia lipolytica. Eur. J. Lipid Sci. Technol. 2003, 105:651-655.
17. Papanikolaou S., Komaitis M., Aggelis G. Single cell oil (SCO) production by Mortierella isabellina grown on high-sugar content media. Bioresour. Technol. 2004, 95:287-291.
18. Papanikolaou S., Chevalot I., Galiotou-Panayotou M., Komaitis M., Marc I., Aggelis G. Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica. Electron. J. Biotechnol. 2007, 10:1-11.
19. Papanikolaou S., Aggelis G. Biotechnological valorization of biodiesel derived glycerol waste through production of single cell oil and citric acid by Yarrowia lipolytica. Lipid Technol. 2009, 21:83-87.
20. Rouwenhorst R.J., Hensing M., Verbakel J., Scheffers W.A., Van Dijken J.P. Structure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556. Appl. Environ. Microbiol. 1990, 56:3337-3345.
21. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, Beijing, pp. 367-370 (Chinese translating ed.). second ed.
22. Spiro R.G. Analysis of sugars found in glycoproteins. Methods Enzymol. 1966, 8:3-26.
23. Wang F., Yue L.X., Wang L., Madzak C., Li J., Wang X.H., Chi Z.M. Genetic modification of the marine-derived yeast Yarrowia lipolytica with high-protein content using a GPI-anchor-fusion expression system. Biotechnol. Prog. 2009, 25:1297-1303.
24. Xuan J.M., Fournier P., Gaillardin C. Cloning of the LYS5 gene encoding saccharopine dehydrogenase. Curr. Genet. 1988, 14:15-21.
25. Yue L.X., Chi Z.M., Wang L., Liu J., Madzak C., Li J. Construction of a new plasmid for surface display on cells of Yarrowia lipolytica. J. Microbiol. Methods 2008, 72:116-123.
26. Zhang T., Gong F., Chi Z., Liu G.L., Chi Z.M., Sheng J., Li J., Wang X.H. Cloning and characterization of the inulinase gene from a marine yeast Pichia guilliermondii and its expression in Pichia pastoris. Antonie van Leeuwenhoek 2009, 95:13-22.
27. Zhang T., Chi Z.M., Sheng J. A highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a potentially useful for single-cell protein production and its nutritive components. Mar. Biotechnol. 2009, 11:280-286.
28. Zhao C.H., Zhang T., Li M., Chi Z.M. Single cell oil production from hydrolysates of inulin and extract of tubers of Jerusalem artichoke by Rhodotorula mucilaginosa TJY15a. Proc. Biochem. 2010, 45:1121-1126.