Genetic modification of the marine-derived yeast yarrowia lipolytica with high-protein content using a GPI-anchor-fusion expression system

Biotechnology Progress

Volumn 25, Issue 5, 2009, Pages 1297-1303

  Wang, Fang ^a   Yue, Lixi ^a   Wang, Lin ^a   Li, Jing ^a   Wang, Xianghong ^a   Chi, Zhenming ^a   Madzak, Catherine ^b  

^a OCEAN UNIVERSITY OF CHINA   (China)

^b CNRS   (France)

Author keywords

Genetic modification; Marine yeasts; Single cell protein; Surface display; Y. lipolytica SWJ 1b

Indexed keywords

GENETIC MODIFICATION; MARINE YEASTS; SINGLE CELL PROTEIN; SURFACE DISPLAY; Y. LIPOLYTICA SWJ-1B;

BIOCHEMISTRY; FATTY ACIDS; GENE ENCODING; GENETIC ENGINEERING; PROTEINS; YEAST;

CELL MEMBRANES;

AMINO ACID; ENHANCED GREEN FLUORESCENT PROTEIN; FATTY ACID; FUNGAL PROTEIN; GLYCOSYLPHOSPHATIDYLINOSITOL; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; URACIL;

ARTICLE; CYTOLOGY; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; YARROWIA;

AMINO ACIDS; CLONING, MOLECULAR; FATTY ACIDS; FUNGAL PROTEINS; GLYCOSYLPHOSPHATIDYLINOSITOLS; GREEN FLUORESCENT PROTEINS; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; URACIL; YARROWIA;

YARROWIA LIPOLYTICA;

EID: 70350161418     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.235     Document Type: Article

References (20)

1. Gao LM, Chi ZM, Sheng J, Ni XM, Wang L. Single-cell protein production from Jerusalem artichoke extract by a recently isolated marine yeast Cryptococcus aureus G7a and its nutritive analysis. Appl Microbiol Biotechnol. 2007;77:825-832.
2. Ravindra AP. Value-added food: single cell protein. Biotechnol Adv. 2000;18:459-479.
3. Sheng J, Chi ZM, Li J, Gao LM, Gong F. 2007. Inulinase production by the marine yeast Cryptococcus aureus G7a and inulin hydrolysis by the crude inulinase. Proc Biochem. 2007;42: 805-811. (Pubitemid 46528340)
4. Maharajh D, Roth R, Lalloo R, Simpson C, Mitra R, Görgens J, Ramchuran S. Multi-copy expression and fed-batch production of Rhodotorula araucariae epoxide hydrolase in Yarrowia lipolytica. Appl Microbiol Biotechnol. 2008;79:235-244.
5. Chi ZM, Liu J, Ji JR, Meng ZL. Enhanced conversion of soluble starch to trehalose by a mutant of Saccharomycopsis fibuligera sdu. J Biotechnol. 2003;102:135-141.
6. Rhishipal R, Philip R. Selection of marine yeasts for the generation of single cell protein from prawn-shell. Bioresour Technol. 1998;65:255-266.
7. Brown MR, Barrett SM, Volkman JK, Nearhos SP, Nell JA. Biochemical composition of new yeasts and bacteria evaluated as food for bivalve aquaculture. Aquaculture 1996;143:341-360.
8. Zhang T, Chi ZM, Sheng J. A highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a potentially useful for single-cell protein production and its nutritive components. Mar Biotechnol. 2009;11:280-286.
9. Oswal N, Sarma PM, Zinjarde SS, Pant A. Palm oil mill effluent treatment by a tropical marine yeast. Bioresour Technol. 2002;85:35-37.
10. Madzak C, Gaillardin C, Beckerich JM. Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. J Biotechnol. 2004;109:63-81.
11. Zhu KL, Chi ZM, Li J, Zhang FL, Li MJ, Yasoda HN, Wu LF. The surface display of haemolysin from Vibrio harveyi on yeast cells and their potential applications as live vaccine in marine fish. Vaccine 2006;24:6046-6052.
12. Yue LX, Chi ZM, Wang L, Liu J, Madzak C, Li J, Wang XH. Construction of a new plasmid for surface display on cells of Yarrowia lipolytica. J Microbiol Methods. 2008;78:116-123.
13. Ni XM, Yue LX, Chi ZM, Li J, Wang XH, Madzak C. Alkaline protease gene cloning from the marine yeast Aureobasidium pullulans HN2-3 and the protease surface display on Yarrowia lipolytica for bioactive peptide production. Mar Biotechnol. 2009;11:81-89.
14. Chi ZM, Liu J, Zhang W. Trehalose accumulation from soluble starch by Saccharomycopsis fibuligera sdu. Enzyme Microb Technol. 2001;28:240-245.
15. Strickland JDH, Parsons TR. Kjehldahl method with ninhydrin finish (low levels). In: Stevenson JC, editor. A Partical Handbook of Seawater Analysis. Bull: Fisheries Research Board of Canada; 1973:227-236.
16. Sambrook J, Fritsch EF, Maniatis T. Preparation and analysis of eukaryotic genomic DNA. In: Sambrook J, Fritscch EF, Maniatis T, editors. Molecular Cloning: A Laboratory Manual. Beijing: Cold Spring Harbor Laboratory Press; 1989:367-370 (Chinese translating ed.).
17. Chi ZM, Ma CL, Wang P, Li HF. Optimization of medium and cultivation conditions for alkaline protease production by the marine yeast Aureobasidium pullulans. Bioresour Technol. 2007;98:534-538.
18. Praphailong WM, Gestel MV, Fleet GH, Heard GM. Evaluation of the Biolog system for the identification of food and beverage yeasts. Lett Appl Microbiol. 1997;24:455-459.
19. Xuan JM, Fournier P, Gaillardin C. Cloning of the LYS5 gene encoding saccharopine dehydrogenase. Curr Genet. 1988;14:15-21.
20. Ratledge C. Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie. 2004;86:807-815.