Possible misinterpretation of the mode of action of therapeutic antibodies in vitro: Homotypic adhesion and flow cytometry result in artefactual direct cell death

Blood

Volumn 116, Issue 17, 2010, Pages 3372-3373

  Golay, Josée ^a   Bologna, Luca ^a   André, Pierre Alain ^b   Buchegger, Franz ^b   Mach, Jean Pierre ^c   Boumsell, Laurence ^d,e   Introna, Martino ^a  

^a OSPEDALI RIUNITI   (Italy)

^b LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^c UNIVERSITY OF LAUSANNE   (Switzerland)

^d INSTITUT COCHIN   (France)

^e MAT Biopharma   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALEMTUZUMAB; ANTIBODY; BK 267 ANTIGEN; CD20 B1 ANTIBODY; HLA ANTIGEN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY GA101; TOSITUMOMAB; TRASTUZUMAB; UNCLASSIFIED DRUG;

CANCER CELL CULTURE; CELL AGGREGATION; CELL DEATH; CELL POPULATION; CHRONIC LYMPHATIC LEUKEMIA; CONTROLLED STUDY; DRUG MECHANISM; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; LETTER; LYMPHOMA; PRIORITY JOURNAL;

EID: 78049394216     PISSN: 00064971     EISSN: 15280020     Source Type: Journal    
DOI: 10.1182/blood-2010-06-289736     Document Type: Letter

References (5)

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2. Mössner E, Brünker P, Moser S, et al. Increasing the efficacy of CD20 antibody therapy through the engineering of a new type II anti-CD20 antibody with enhanced direct and immune effector cell-mediated B-cell cytotoxicity. Blood. 2010;115(22):4393-4402.
3. Alduaij W, Potluri S, Ivanov A, et al. New generation anti-CD20 antibody (GA101) evokes homotypic adhesion and actin-dependent, lysosome-mediated cell death in B-cell lymphoma [abstract]. Blood (ASH Annual Meeting Abstracts). 2009;114(22):302a. Abstract 725.
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5. Beers SA, French RR, Chan HT, et al. Antigenic modulation limits the efficacy of anti-CD20 antibodies: implications for antibody selection. Blood. 2010; 115(25):5191-5201.