FRAP analysis of membrane-associated proteins: Lateral diffusion and membrane-cytoplasmic exchange

Biophysical Journal

Volumn 99, Issue 8, 2010, Pages 2443-2452

  Goehring, Nathan W ^a   Chowdhury, Debanjan ^b,c   Hyman, Anthony A ^a   Grill, Stephan W ^a,b  

^a MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^b MAX PLANCK INSTITUT FÜR PHYSIK KOMPLEXER SYSTEME   (Germany)

^c INDIAN INSTITUTE OF TECHNOLOGY KANPUR   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CAENORHABDITIS ELEGANS;

EID: 78049324117     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2010.08.033     Document Type: Article

References (21)

1. Rabut, G., and J. Ellenberg. 2005. Photobleaching techniques to study mobility and molecular dynamics of proteins in live cells: FRAP, iFRAP and FLIP. In Live Cell Imaging: A Laboratory Manual, Vol.1. R. D. Goldman, and D. L. Spector, editors. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 101-127.
2. Sprague, B. L., and J. G. McNally. 2005. FRAP analysis of binding: Proper and fitting. Trends Cell Biol. 15:84-91.
3. Poo, M., and R. A. Cone. 1974. Lateral diffusion of rhodopsin in the photoreceptor membrane. Nature. 247:438-441.
4. Liebman, P. A., and G. Entine. 1974. Lateral diffusion of visual pigment in photoreceptor disk membranes. Science. 185:457-459.
5. Axelrod, D., D. E. Koppel, ⋯, W. W. Webb. 1976. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J. 16:1055-1069.
6. McNally, J. G. 2008. Quantitative FRAP in analysis of molecular binding dynamics in vivo. Methods Cell Biol. 85:329-351.
7. Soumpasis, D. M. 1983. Theoretical analysis of fluorescence photobleaching recovery experiments. Biophys. J. 41:95-97.
8. Ellenberg, J., E. D. Siggia, ⋯, J. Lippincott-Schwartz. 1997. Nuclear membrane dynamics and reassembly in living cells: Targeting of an inner nuclear membrane protein in interphase and mitosis. J. Cell Biol. 138:1193-1206.
9. Coscoy, S., F. Waharte, ⋯, F. Amblard. 2002. Molecular analysis of microscopic ezrin dynamics by two-photon FRAP. Proc. Natl. Acad. Sci. USA. 99:12813-12818.
10. Oancea, E., M. N. Teruel,., T. Meyer. 1998. Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. J. Cell Biol. 140:485-498.
11. Hammond, G. R. V., Y. Sim,., R. F. Irvine. 2009. Reversible binding and rapid diffusion of proteins in complex with inositol lipids serves to coordinate free movement with spatial information. J. Cell Biol. 184:297-308.
12. Sprague, B. L., R. L. Pego, ⋯, J. G. McNally. 2004. Analysis of binding reactions by fluorescence recovery after photobleaching. Biophys. J. 86:3473-3495.
13. Weiss, M. 2004. Challenges and artifacts in quantitative photobleaching experiments. Traffic. 5:662-671.
14. Seiffert, S., and W. Oppermann. 2005. Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope. J. Microsc. 220:20-30.
15. Hauser, G. I., S. Seiffert, and W. Oppermann. 2008. Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope. Part II. Multiple diffusion processes. J. Microsc. 230: 353-362.
16. Beaudouin, J., F. Mora-Bermúdez, ⋯, J. Ellenberg. 2006. Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins. Biophys. J. 90:1878-1894.
17. Sprague, B. L., F. Müller,., J. G. McNally. 2006. Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching. Biophys. J. 91:1169-1191.
18. Gradshteyn, I. S., and I. M. Ryzhik. 2007. Table of Integrals, Series and Products, 7th Ed. Academic Press, New York.
19. Audhya, A., F. Hyndman,., K. Oegema. 2005. A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans. J. Cell Biol. 171:267-279.
20. Petrásek, Z., C. Hoege, ⋯, P. Schwille. 2008. Characterization of protein dynamics in asymmetric cell division by scanning fluorescence correlation spectroscopy. Biophys. J. 95:5476-5486.
21. Brough, D., F. Bhatti, and R. F. Irvine. 2005. Mobility of proteins associated with the plasma membrane by interaction with inositol lipids. J. Cell Sci. 118:3019-3025.