BchJ and BchM interact in a 1 1 ratio with the magnesium chelatase BchH subunit of Rhodobacter capsulatus

FEBS Journal

Volumn 277, Issue 22, 2010, Pages 4709-4721

  Sawicki, Artur ^a   Willows, Robert D ^a  

^a MACQUARIE UNIVERSITY   (Australia)

Author keywords

Bacteriochlorophyll biosynthesis; BchJ; BchM; Magnesium chelatase; O methyltransferase

Indexed keywords

METHYLTRANSFERASE; PROTOPORPHYRIN; S ADENOSYLMETHIONINE;

ARTICLE; EQUILIBRIUM CONSTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN INTERACTION; RHODOBACTER CAPSULATUS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; LYASES; MAGNESIUM; METHYLTRANSFERASES; MOLECULAR STRUCTURE; POLYSORBATES; PROTEIN SUBUNITS; PROTOPORPHYRINS; RHODOBACTER CAPSULATUS; SURFACE-ACTIVE AGENTS;

CYANOBACTERIA; PHOTOBACTERIA; RHODOBACTER; RHODOBACTER CAPSULATUS;

EID: 78049316320     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2010.07877.x     Document Type: Article

References (50)

1. Chew AG & Bryant DA (2007) Chlorophyll biosynthesis in bacteria: the origins of structural and functional diversity. Annu Rev Microbiol 61, 113-129.
2. Willows RD & Kriegel AM (2009) Biosynthesis of bacteriochlorophyll in purple bacteria. In The Purple Phototropic Bacteria (Hunter CN, Daldal F, Thurnauer MC & Beatty JT eds), pp 57-79. Springer Science, Dordrecht.
3. Walker C & Willows R (1997) Mechanism and regulation of Mg-chelatase. Biochem J 327, 321-333.
4. Willows RD & Hansson M (2003) Mechanism, structure and regulation of magnesium chelatase. In The Porphyrin Handbook II (Kadish KM, Smith K & Guilard R eds), pp 1-48. Academic Press, San Diego, CA, USA.
5. Viney J, Davison P, Hunter C & Reid J (2007) Direct measurement of metal-ion chelation in the active site of the AAA+ ATPase magnesium chelatase. Biochemistry 46, 12788-12794.
6. Bollivar D, Kadish KM, Smith K & Guilard R (2003) Regulatory mechanisms of eukaryotic tetrapyrrole biosynthesis. In The Porphyrin Handbook II (Kadish KM, Smith K & Guilard R eds), pp. 49-70. Academic Press, San Diego, CA, USA.
7. Rissler H, Collakova E, DellaPenna D, Whelan J & Pogson B (2002) Chlorophyll biosynthesis. Expression of a second chl I gene of magnesium chelatase in Arabidopsis supports only limited chlorophyll synthesis. Plant Physiol 128, 770-779.
8. Apchelimov AA, Soldatova OP, Ezhova TA, Grimm B & Shestakov SV (2007) The analysis of the ChlI 1 and ChlI 2 genes using acifluorfen-resistant mutant of Arabidopsis thaliana. Planta 225, 935-943.
9. Kobayashi K, Mochizuki N, Yoshimura N, Motohashi K, Hisabori T & Masuda T (2008) Functional analysis of Arabidopsis thaliana isoforms of the Mg-chelatase CHLI subunit. Photochem Photobiol Sci 7, 1188-1195.
10. Huang YS & Li HM (2009) Arabidopsis CHLI2 can substitute for CHLI1. Plant Physiol 150, 636-645.
11. Johnson ET & Schmidt-Dannert C (2008) Characterization of three homologs of the large subunit of the magnesium chelatase from Chlorobaculum tepidum and interaction with the magnesium protoporphyrin IX methyltransferase. J Biol Chem 283, 27776-27784.
12. Lundqvist J, Elmlund H, Wulff RP, Berglund L, Elmlund D, Emanuelsson C, Hebert H, Willows RD, Hansson M, Lindahl M et al. (2010) ATP-induced conformational dynamics in the AAA+ motor unit of magnesium chelatase. Structure 18, 354-365.
13. Willows R, Hansson A, Birch D, Al-Karadaghi S & Hansson M (2004) EM single particle analysis of the ATP-dependent BchI complex of magnesium chelatase: an AAA+ hexamer. J Struct Biol 146, 227-233.
14. Axelsson E, Lundqvist J, Sawicki A, Nilsson S, Schroder I, Al-Karadaghi S, Willows R & Hansson M (2006) Recessiveness and dominance in barley mutants deficient in Mg-chelatase subunit D, an AAA protein involved in chlorophyll biosynthesis. Plant Cell 18, 3606-3616.
15. Sirijovski N, Lundqvist J, Rosenback M, Elmlund H, Al-Karadaghi S, Willows RD & Hansson M (2008) Substrate-binding model of the chlorophyll biosynthetic magnesium chelatase BchH subunit. J Biol Chem 283, 31294-31302.
16. Karger GA, Reid JD & Hunter CN (2001) Characterization of the binding of deuteroporphyrin IX to the magnesium chelatase H subunit and spectroscopic properties of the complex. Biochemistry 40, 9291-9299.
17. Willows RD, Gibson LCD, Kanangara CG, Hunter CN & Von WD (1996) Three separate proteins constitute the magnesium chelatase of Rhodobacter sphaeroides. Eur J Biochem 235, 438-443.
18. Sawicki A & Willows RD (2008) Kinetic analyses of the magnesium chelatase provide insights into the mechanism, structure, and formation of the complex. J Biol Chem 283, 31294-31302.
19. Jensen PE, Gibson LC & Hunter CN (1999) ATPase activity associated with the magnesium-protoporphyrin IX chelatase enzyme of Synechocystis PCC6803: evidence for ATP hydrolysis during Mg2+ insertion, and the MgATP-dependent interaction of the ChlI and ChlD subunits. Biochem J 339, 127-134.
20. Larkin RM, Alonso JM, Ecker JR & Chory J (2003) GUN4, a regulator of chlorophyll synthesis and intracellular signaling. Science 299, 902-906.
21. Davison P, Schubert H, Reid J, Iorg C, Heroux A, Hill C & Hunter C (2005) Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis. Biochemistry 44, 7603-7612.
22. Verdecia M, Larkin R, Ferrer J, Riek R, Chory J & Noel J (2005) Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding. PLoS Biol 3, e151.
23. Adhikari ND, Orler R, Chory J, Froehlich JE & Larkin RM (2009) Porphyrins promote the association of GENOMES UNCOUPLED 4 and a Mg-chelatase subunit with chloroplast membranes. J Biol Chem 284, 24783-24796.
24. Nakayama M, Masuda T & Takamiya K-I (1998) Cloning and expression of the soybean Ch1H gene encoding a subunit of Mg-chelatase and localization of the Mg2+ concentration-dependent Ch1H protein within the chloroplast. Plant Cell Physiol 39, 275.
25. Luo M, Weinstein J & Walker C (1999) Magnesium chelatase subunit D from pea: characterization of the cDNA, heterologous expression of an enzymatically active protein and immunoassay of the native protein. Plant Mol Biol 41, 721-731.
26. Suzuki JY & Bauer CE (1995) Altered monovinyl and divinyl protochlorophyllide pools in bchJ mutants of Rhodobacter capsulatus. Possible monovinyl substrate discrimination of light-independent protochlorophyllide reductase. J Biol Chem 270, 3732-3740.
27. Hinchigeri S & Richards W (1982) The reaction mechanism of S-adenosyl-L-methionine:magnesium protoporphyrin methyltransferase from Euglena gracilis. Photosynthetica 16, 554-560.
28. Hinchigeri S, Nelson D & Richards W (1984) The purification and reaction mechanism of S-adenosyl-L-methionine:magnesium protoporphyrin methyltransferase from Rhodopseudomonas sphaeroides. Photosynthetica 18, 168-178.
29. Yee WC, Eglsaer SJ & Richards WR (1989) Confirmation of a ping-pong mechanism for S-adenosyl-L-methionine:magnesium protoporphyrin methyltransferase of etiolated wheat by an exchange reaction. Biochem Biophys Res Commun 162, 483-490.
30. Shepherd M, Reid JD & Hunter CN (2003) Purification and kinetic characterization of the magnesium protoporphyrin IX methyltransferase from Synechocystis PCC6803. Biochem J 371, 351-360.
31. Shepherd M & Hunter CN (2004) Transient kinetics of the reaction catalysed by magnesium protoporphyrin IX methyltransferase. Biochem J 382, 1009-1013.
32. Sawicki A & Willows R (2007) S-adenosyl-L-methionine:magnesium-protoporphyrin IX O-methyltransferase from Rhodobacter capsulatus: mechanistic insights and stimulation with phospholipids. Biochem J 406, 469-478.
33. Bollivar DW (2003) Intermediate steps in chlorophyll biosynthesis: methylation and cyclization. Porphyrin Handbook 13, 49-69.
34. Dorgan KM, Wooderchak WL, Wynn DP, Karschner EL, Alfaro JF, Cui Y, Zhou ZS & Hevel JM (2006) An enzyme-coupled continuous spectrophotometric assay for S-adenosylmethionine-dependent methyltransferases. Anal Biochem 350, 249-255.
35. McLean S & Hunter CN (2009) An enzyme-coupled continuous spectrophotometric assay for magnesium protoporphyrin IX methyltransferases. Anal Biochem 394, 223-228.
36. Van Wilder V, De Brouwer V, Loizeau K, Gambonnet B, Albrieux C, Van Der Straeten D, Lambert WE, Douce R, Block MA, Rebeille F et al. (2009) C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is dependent on the folate status. New Phytol 182, 137-145.
37. Gorchein A (1972) Magnesium protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Studies with whole cells. Biochem J 127, 97-106.
38. Shepherd M, McLean S & Hunter C (2005) Kinetic basis for linking the first two enzymes of chlorophyll biosynthesis. FEBS J 272, 4532-4539.
39. Hinchigeri SB, Hundle B & Richards WR (1997) Demonstration that the BchH protein of Rhodobacter capsulatus activates S-adenosyl-l-methionine:magnesium protoporphyrin IX methyltransferase. FEBS Lett 407, 337-342.
40. Alawady A & Grimm B (2005) Tobacco Mg protoporphyrin IX methyltransferase is involved in inverse activation of Mg porphyrin and protoheme synthesis. Plant J 41, 282-290.
41. Alawady A, Reski R, Yaronskaya E & Grimm B (2005) Cloning and expression of the tobacco CHLM sequence encoding Mg protoporphyrin IX methyltransferase and its interaction with Mg chelatase. Plant Mol Biol 57, 679-691.
42. Shelnutt JA, Song X-Z, Ma J-G, Jia S-L, Jentzen W & Medforth CJ (1998) Nonplanar porphyrins and their significance in proteins. Chem Soc Rev 27, 31-42.
43. Kawamura-Konishi Y, Kihara H & Suzuki H (1988) Reconstitution of myoglobin from apoprotein and heme, monitored by stopped-flow absorption, fluorescence and circular dichroism. Eur J Biochem 170, 589-595.
44. Jensen P, Gibson L, Shephard F, Smith V & Hunter C (1999) Introduction of a new branch point in tetrapyrrole biosynthesis in Escherichia coli by co-expression of genes encoding the chlorophyll-specific enzymes magnesium chelatase and magnesium protoporphyrin methyltransferase. FEBS Lett 455, 349-354.
45. Neugebauer JM (1990) Detergents: an overview. Methods Enzymol 182, 239-253.
46. Aizawa H (2009) Morphology of polysorbate 80 (Tween 80) micelles in aqueous 1, 4-dioxane solutions. J Appl Crystallogr 42, 592-596.
47. De Campo L, Yaghmur A, Garti N, Leser ME, Folmer B & Glatter O (2004) Five-component food-grade microemulsions: structural characterization by SANS. J Colloid Interface Sci 274, 251-267.
48. Alberti M, Burke DH, Hearst JE, Blankenship RE, Madigan MT & Bauer CE (1995) Structure and sequence of the photosynthetic gene cluster. In Anoxygenic Photosynthetic Bacteria (Blankenship RE, Madigan MT & Bauer CE eds), pp 1083-1106. Kluwer Academic Publishers, Dordrecht, The Netherlands.
49. Willows R & Beale S (1998) Heterologous expression of the Rhodobacter capsulatus BchI, -D, and -H genes that encode magnesium chelatase subunits and characterization of the reconstituted enzyme. J Biol Chem 273, 34206-34213.
50. Patton C, Thompson S & Epel D (2004) Some precautions in using chelators to buffer metals in biological solutions. Cell Calcium 35, 427-431.