Heterologous expression and extracellular secretion of cellulolytic enzymes by zymomonas mobilis

Applied and Environmental Microbiology

Volumn 76, Issue 19, 2010, Pages 6360-6369

  Linger, Jeffrey G ^a   Adney, William S ^b   Darzins, Al ^a,c  

^a National Bioenergy Center   (United States)

^b Chemical and Biosciences Center   (United States)

^c NATIONAL RENEWABLE ENERGY LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDOTHERMUS CELLULOLYTICUS; BIOFUEL PRODUCTION; CARBOXY-METHYL CELLULOSE; CELL PROTEINS; CELLULOLYTIC ENZYME; CONSOLIDATED BIOPROCESSING; EXTRACELLULAR ACTIVITY; EXTRACELLULAR SECRETION; HETEROLOGOUS EXPRESSION; INTRACELLULAR POOL; LIGNOCELLULOSIC BIOMASS; MONOMERIC SUGARS; PERIPLASMIC SPACE; SUBCELLULAR LOCALIZATIONS; ZYMOMONAS MOBILIS;

BAST FIBERS; CATALYSTS; ENZYME ACTIVITY; ETHANOL; FERMENTATION; LAKES; SUGAR (SUCROSE);

ENZYMES;

BACTERIAL DNA; CARBOXYMETHYLCELLULOSE; CELLULASE; HYBRID PROTEIN; SIGNAL PEPTIDE;

BACTERIUM; BIOFUEL; CELLULOSE; ENZYME ACTIVITY; ETHANOL; FERMENTATION; LIGNIN; MICROBIAL COMMUNITY; PROTEIN; SECRETION; SUGAR;

ACTINOMYCETALES; ARTICLE; BIOTECHNOLOGY; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN TRANSPORT; SECRETION; ZYMOMONAS;

ACTINOMYCETALES; BIOTECHNOLOGY; CARBOXYMETHYLCELLULOSE SODIUM; CELLULASES; DNA, BACTERIAL; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; ZYMOMONAS;

BAST FIBERS; CATALYSTS; CELLS; ENZYMES; ETHANOL; FERMENTATION; LAKES; MONOMERS; SUGAR;

ACIDOTHERMUS CELLULOLYTICUS; ZYMOMONAS MOBILIS;

EID: 78049292737     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00230-10     Document Type: Article

References (48)

1. Aldrich, H. C., L. McDowell, M. F. Barbosa, L. P. Yomano, R. K. Scopes, and L. O. Ingram. 1992. Immunocytochemical localization of glycolytic and fermentative enzymes in Zymomonas mobilis. J. Bacteriol. 174:4504-4508.
2. Arfman, N., V. Worrell, and L. O. Ingram. 1992. Use of the tac promoter and lacIq for the controlled expression of Zymomonas mobilis fermentative genes in Escherichia coli and Zymomonas mobilis. J. Bacteriol. 174:7370-7378.
3. Arnold, K., L. Bordoli, J. Kopp, and T. Schwede. 2006. The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195-201.
4. Bendtsen, J. D., H. Nielsen, G. von Heijne, and S. Brunak. 2004. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340:783-795.
5. Brestic-Goachet, N., P. Gunasekaran, B. Cami, and J. Baratti. 1990. Transfer and expression of a Bacillus licheniformis alpha-amylase gene in Zymomonas mobilis. Arch. Microbiol. 153:219-225.
6. Brestic-Goachet, N., P. Gunasekaran, B. Cami, and J. C. Baratti. 1989. Transfer and expression of an Erwinia chrysanthemi cellulase gene in Zymomonas mobilis. J. Gen. Microbiol. 135:893-902.
7. Byun, M. O. K., J. B. Kaper, and L. O. Ingram. 1986. Construction of a new vector for the expression of foreign genes in Zymomonas mobilis. J. Ind. Microbiol. 1:9-15.
8. Carere, C. R., R. Sparling, N. Cicek, and D. B. Levin. 2008. Third generation biofuels via direct cellulose fermentation. Int. J. Mol. Sci. 9:1342-1360.
9. Carey, V. C., S. K. Walia, and L. O. Ingram. 1983. Expression of a lactose transposon (Tn951) in Zymomonas mobilis. Appl. Environ. Microbiol. 46: 1163-1168.
10. Deanda, K., M. Zhang, C. Eddy, and S. Picataggio. 1996. Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering. Appl. Environ. Microbiol. 62:4465-4470.
11. Ding, S. Y., W. S. Adney, T. B. Vinzant, M. E. Himmel, and S. R. Decker. June 2006. Thermal tolerant cellulase from Acidothermus cellulolyticus. U.S. patent 7,059,993.
12. Franden, M. A., P. T. Pienkos, and M. Zhang. 2009. Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis. J. Biotechnol. 144:259-267.
13. Himmel, M. E., S. Y. Ding, D. K. Johnson, W. S. Adney, M. R. Nimlos, J. W. Brady, and T. D. Foust. 2007. Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315:804-807.
14. Kumar, R., S. Singh, and O. V. Singh. 2008. Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. J. Ind. Microbiol. Biotechnol. 35:377-391.
15. Lee, J. H., R. J. Pagan, and P. L. Rogers. 1983. Continuous simultaneous saccharification and fermentation of starch using Zymomonas mobilis. Biotechnol. Bioeng. 25:659-669.
16. Lee, K. J., M. Lefebvre, D. E. Tribe, and P. L. Rogers. 1980. High productivity ethanol fermentations with Zymomonas mobilis using continuous cell recycle. Biotechnol. Lett. 2:487-492.
17. Lee, K. J., M. L. Skotnicki, D. E. Tribe, and P. L. Rogers. 1980. Kinetic studies on a highly productive strain of Zymomonas mobilis. Biotechnol. Lett. 2:339-344.
18. Lee, K. J., D. E. Tribe, and P. L. Rogers. 1979. Ethanol production by Zymomonas mobilis in continuous culture at high glucose concentrations. Biotechnol. Lett. 1:421-426.
19. Lee, P. A., D. Tullman-Ercek, and G. Georgiou. 2006. The bacterial twinarginine translocation pathway. Annu. Rev. Microbiol. 60:373-395.
20. Lejeune, A., D. E. Eveleigh, and C. Colson. 1988. Expression of an endoglucanase gene of Pseudomonas fluorescens var. cellulosa in Zymomonas mobilis. FEMS Microbiol. Lett. 49:363-366.
21. Lynd, L. R. 1996. Overview and evaluation of fuel ethanol from cellulosic biomass: technology, economics, the environment, and policy. Annu. Rev. Energy Environ. 21:403-465.
22. Lynd, L. R., W. H. van Zyl, J. E. McBride, and M. Laser. 2005. Consolidated bioprocessing of cellulosic biomass: an update. Curr. Opin. Biotechnol. 16: 577-583.
23. Lynd, L. R., P. J. Weimer, W. H. van Zyl, and I. S. Pretorius. 2002. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66:506-577.
24. Makrides, S. C. 1996. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60:512-538.
25. McMillan, J. D., M. M. Newman, D. W. Templeton, and A. Mohagheghi. 1999. Simultaneous saccharification and cofermentation of dilute-acid pretreated yellow poplar hardwood to ethanol using xylose-fermenting Zymomonas mobilis. Appl. Biochem. Biotechnol. 77-79:649-665.
26. Mejia, J. P., M. E. Burnett, H. An, W. O. Barnell, K. F. Keshav, T. Conway, and L. O. Ingram. 1992. Coordination of expression of Zymomonas mobilis glycolytic and fermentative enzymes: a simple hypothesis based on mRNA stability. J. Bacteriol. 174:6438-6443.
27. Mergulhao, F. J., D. K. Summers, and G. A. Monteiro. 2005. Recombinant protein secretion in Escherichia coli. Biotechnol. Adv. 23:177-202.
28. Misawa, N., T. Okamoto, and K. Nakamura. 1988. Expression of a cellulase gene in Zymomonas mobilis. J. Biotechnol. 7:167-178.
29. Missiakas, D., and S. Raina. 1997. Protein folding in the bacterial periplasm. J. Bacteriol. 179:2465-2471.
30. Omullan, P. J., T. Chase, and D. E. Eveleigh. 1992. Purification and some properties of extracellular invertase-B from Zymomonas mobilis. Appl. Microbiol. Biotechnol. 38:341-346.
31. Rajnish, K. N., G. M. K. Choudhary, and P. Gunasekaran. 2008. Functional characterization of a putative endoglucanase gene in the genome of Zymomonas mobilis. Biotechnol. Lett. 30:1461-1467.
32. Rogers, P. L., Y. J. Jeon, K. J. Lee, and H. G. Lawford. 2007. Zymomonas mobilis for fuel ethanol and higher value products. Adv. Biochem. Eng. Biotechnol. 108:263-288.
33. Rogers, P. L., K. J. Lee, and D. E. Tribe. 1980. High productivity ethanol fermentations with Zymomonas mobilis. Process Biochem. 15:7-11.
34. Rogers, P. L., K. J. Lee, and D. E. Tribe. 1979. Kinetics of alcohol production by Zymomonas mobilis at high sugar concentrations. Biotechnol. Lett. 1:165-170.
35. Sandkvist, M. 2001. Biology of type II secretion. Mol. Microbiol. 40:271-283.
36. Skotnicki, M. L., K. J. Lee, D. E. Tribe, and P. L. Rogers. 1981. Comparison of ethanol production by different Zymomonas strains. Appl. Environ. Microbiol. 41:889-893.
37. Spangler, D. J., and G. H. Emert. 1986. Simultaneous saccharification fermentation with Zymomonas mobilis. Biotechnol. Bioeng. 28:115-118.
38. Swings, J., and J. Deley. 1977. Biology of Zymomonas. Bacteriol. Rev. 41: 1-46.
39. Taylor, L. E., II, B. Henrissat, P. M. Coutinho, N. A. Ekborg, S. W. Hutcheson, and R. M. Weiner. 2006. Complete cellulase system in the marine bacterium Saccharophagus degradans strain 2-40T. J. Bacteriol. 188: 3849-3861.
40. Thomas, S. R., R. A. Laymon, and M. E. Himmel. July 1996. Gene coding for the E1 endoglucanase. U.S. patent 5,536,655.
41. Villalobos, A., J. E. Ness, C. Gustafsson, J. Minshull, and S. Govindarajan. 2006. Gene Designer: a synthetic biology tool for constructing artificial DNA segments. BMC Bioinformatics 7:285.
42. Weiss, A., C. Klein, B. Woodman, K. Sathasivam, M. Bibel, E. Regulier, G. P. Bates, and P. Paganetti. 2008. Sensitive biochemical aggregate detection reveals aggregation onset before symptom development in cellular and murine models of Huntington's disease. J. Neurochem. 104:846-858.
43. Yablonsky, M. D., A. E. Goodman, N. Stevnsborg, O. G. Delima, J. O. F. Demorais, H. G. Lawford, P. L. Rogers, and D. E. Eveleigh. 1988. Zymomonas mobilis CP4: a clarification of strains via plasmid profiles. J. Biotechnol. 9:71-79.
44. Yanase, H., J. Kurii, and K. Tonomura. 1986. Construction of a promotercloning vector in Zymomonas mobilis. Agric. Biol. Chem. 50:2959-2961.
45. Yanase, H., K. Nozaki, and K. Okamoto. 2005. Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis. Biotechnol. Lett. 27:259-263.
46. Yoon, K. H., S. H. Park, and M. Y. Pack. 1988. Transfer of Bacillus subtilis endo-β-1,4-glucanase gene into Zymomonas anaerobia. Biotechnol. Lett. 10:213-216.
47. Zhang, M., C. Eddy, K. Deanda, M. Finkestein, and S. Picataggio. 1995. Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis. Science 267:240-243.
48. Zhang, M., M. A. Franden, M. Newman, J. Mcmillan, M. Finkelstein, and S. Picataggio. 1995. Promising ethanologens for xylose fermentation. Appl. Biochem. Biotechnol. 51-52:527-536.