On-column refolding purification of DT389-hIL13 recombinant protein expressed in Escherichia coli

Protein Expression and Purification

Volumn 75, Issue 1, 2011, Pages 83-88

  Sun, Weimin ^a   Dai, Xinxian ^a   Zheng, Yanpeng ^a   Wang, Jianwei ^b   Hou, Lingling ^a   Du, Juan ^a   Hu, Hong Gang ^a  

^a BEIJING JIAOTONG UNIVERSITY   (China)

^b INSTITUTE OF PATHOGEN BIOLOGY   (China)

Author keywords

Inclusion bodies; On column refolding; pH gradient; Size exclusion chromatography; Urea gradient

Indexed keywords

INTERLEUKIN 13; RECOMBINANT PROTEIN;

ARTICLE; CELL INCLUSION; CELL SURVIVAL; CHEMISTRY; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; GLIOMA; HUMAN; ISOLATION AND PURIFICATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN REFOLDING; TUMOR CELL LINE; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL LINE, TUMOR; CELL SURVIVAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; GLIOMA; HUMANS; INCLUSION BODIES; INTERLEUKIN-13; PROTEIN REFOLDING; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 78049290398     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.09.009     Document Type: Article

References (25)

1. D. Caput, P. Laurent, M. Kaghad, J.M. Lelias, S. Lefort, N. Vita, and P. Ferrara Cloning and characterization of a specific interleukin (IL)-13 binding protein structurally related to the IL-5 receptor alpha chain J. Biol. Chem. 271 1996 16921 16926
2. W. Debinski, D.M. Gibo, S.W. Hulet, J.R. Connor, and G.Y. Gillespie Receptor for interleukin 13 is a marker and therapeutic target for human high-grade gliomas Clin. Cancer Res. 5 1999 985 990 (Pubitemid 29233211)
3. W. Debinski, N.I. Obiri, S.K. Powers, I. Pastan, and R.K. Puri Human glioma cells overexpress receptors for interleukin 13 and are extremely sensitive to a novel chimeric protein composed of interleukin 13 and Pseudomonas exotoxin Clin. Cancer Res. 1 1995 1253 1258
4. H.Z. Tuo, J.W. Wang, D.X. Wang, J.M. Li, J. Ouyang, and T. Hong Construction and expression of the fusion protein DT389-hIL-13 and its cytotoxicity to glioma cell lines Zhonghua Yi Xue Za Zhi 84 2004 1024 1028
5. S. Misawa, and I. Kumagai Refolding of therapeutic proteins produced in Escherichia coli as inclusion bodies Biopolymers 51 1999 297 307
6. H. Lilie, E. Schwarz, and R. Rudolph Advances in refolding of proteins produced in E. Coli Curr. Opin. Biotechnol. 9 1998 497 501
7. J. Chen, Y. Liu, X. Li, Y. Wang, H. Ding, G. Ma, and Z. Su Cooperative effects of urea and l-arginine on protein refolding Protein Exp. Purif. 66 2009 82 90
8. Z. Gu, M. Weidenhaupt, N. Ivanova, M. Pavlov, B. Xu, Z.-G. Su, and J.-C. Janson Chromatographic methods for the isolation of and refolding of proteins from, Escherichia coli inclusion bodies Protein Exp. Purif. 25 2002 174 179
9. Y. Chen, and S.S.J. Leong Adsorptive refolding of a highly disulfide-bonded inclusion body protein using anion-exchange chromatography J. Chromatogr. A 1216 2009 4877 4886
10. M. Ersoy, S. Matic, and S.R. Schmidt On-column refolding of corticotropin-releasing factor receptor 1 extracellular domain by size exclusion chromatography Biomed. Chromatogr. 23 2009 1121 1128
11. A.K. Srivastava, Y. Sharma, and K.V. Chary Overexpression on-column refolding and isotopic labeling of Hahellin from Hahella chejuensis, a putative member of the betagamma-crystallin superfamily Protein Exp. Purif. 58 2008 269 274
12. M. Li, Z.-G. Su, and J.-C. Janson In vitro protein refolding by chromatographic procedures Protein Exp. Purif. 33 2004 1 10
13. A. Jungbauer, W. Kaar, and R. Schlegl Folding and refolding of proteins in chromatographic beds Curr. Opin. Biotechnol. 15 2004 487 494
14. F. Wang, Y. Liu, J. Li, G. Ma, and Z. Su On-column refolding of consensus interferon at high concentration with guanidine-hydrochloride and polyethylene glycol gradients J. Chromatogr. A 1115 2006 72 80
15. J.Q. Guo, Q.M. Li, J.Y. Zhou, G.P. Zhang, Y.Y. Yang, G.X. Xing, D. Zhao, S.Y. You, and C.Y. Zhang Efficient recovery of the functional IP10-scFv fusion protein from inclusion bodies with an on-column refolding system Protein Exp. Purif. 45 2006 168 174
16. B. Batas, and J.B. Chaudhuri Protein refolding at high concentration using size-exclusion chromatography Biotechnol. Bioeng. 50 1996 16 23
17. X. Fan, D. Xu, B. Lu, J. Xia, and D. Wei Improving the refolding of NTA protein by urea gradient and arginine gradient size-exclusion chromatography J. Biochem. Biophys. Methods 70 2008 1130 1138
18. Z. Gu, Z. Su, and J.C. Janson Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding J. Chromatogr. A 918 2001 311 318
19. Z. Gu, M. Weidenhaupt, N. Ivanova, M. Pavlov, B. Xu, Z.G. Su, and J.C. Janson Chromatographic methods for the isolation of and refolding of proteins from, Escherichia coli inclusion bodies Protein Exp. Purif. 25 2002 174 179
20. Y. Maeda, H. Koga, H. Yamada, T. Ueda, and T. Imoto Effective renaturation of reduced lysozyme by gentle removal of urea Protein Eng. 8 1995 201 205
21. F. Dolder A method for pH-dependent selective reduction of disulfide bonds in human immunoglobulin M using heterocyclic compounds Biochim. Biophys. Acta-Protein Struct. 207 1970 286 296
22. T. Harada, E. Kurimoto, K. Tokuhiro, O. Asami, T. Sakai, D. Nohara, and K. Kato Disulfide bond formation in refolding of thermophilic fungal protein disulfide isomerase J. Biosci. Bioeng. 91 2001 596 598
23. D. Mohanraj, and S. Ramakrishnan Cytotoxic effects of ricin without an interchain disulfide bond: genetic modification and chemical crosslinking studies Biochim. Biophys. Acta-Gen. Subj. 1243 1995 399 406
24. G.-Z. Wang, X.-Y. Dong, and Y. Sun The role of disulfide bond formation in the conformational folding kinetics of denatured/reduced lysozyme Biochem. Eng. J. 46 2009 7 11
25. K.J. Woycechowsky, and R.T. Raines Native disulfide bond formation in proteins Curr. Opin. Chem. Biol. 4 2000 533 539