Molecular cloning of the cellobiose dehydrogenase gene from Trametes versicolor and expression in Pichia pastoris

Enzyme and Microbial Technology

Volumn 34, Issue 1, 2004, Pages 55-63

  Stapleton, P C ^a   O'Brien, M M ^b   O'Callaghan, J ^a   Dobson, A D W ^a  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

Cellobiose dehydrogenase; Heterologous expression; Trametes versicolor; White rot fungus

Indexed keywords

CATALYST ACTIVITY; CLONING; ENZYME KINETICS; GENES; MOLECULAR WEIGHT; PH EFFECTS; THERMODYNAMIC STABILITY;

SUBSTRATE AFFINITY;

ENZYMES;

CELLOBIOSE QUINONE OXIDOREDUCTASE; CYTOCHROME C; GLYCOSIDASE; LACTOSE; METHANOL;

ACIDITY; ALKALINITY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; HETEROLOGOUS EXPRESSION; MOLECULAR CLONING; MOLECULAR MECHANICS; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION; SPECIES COMPARISON; TEMPERATURE DEPENDENCE; TRAMETES VERSICOLOR; WILD TYPE;

FUNGI; PICHIA; PICHIA PASTORIS; TRAMETES; TRAMETES VERSICOLOR;

EID: 0345096537     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2003.08.006     Document Type: Article

References (42)

1. Cameron M.D., Aust S.D. Cellobiose dehydrogenase-an extracellular fungal flavocytochrome. Enz. Microb. Technol. 28:2001;129-138.
2. Roy B.P., Dumonceaux T., Koukoulas A.A., Archibald F.S. Purification and characterization of cellobiose dehydrogenase from the white rot fungus Trametes versicolor. Appl. Environ. Microbiol. 62:1996;4417-4427.
3. Li B., Nagalla S.R., Renganathan V. Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium. Appl. Environ. Microbiol. 62:1996;1329-1335.
4. Coudray M.R., Canevascini G., Meier H. Characterization of a cellobiose dehydrogenase in the celluloytic fungus Sporotrichum (Chrysosporium) thermophile. Biochem. J. 203:1982;277-284.
5. Schmidhalter D.R., Canevascini G. Isolation and characterization of the cellobiose dehydrogenase from the brown-rot fungus Coniophora puteana (Schum ex Fr) Karst. Arch. Biochem. Biophys. 300:1993;559-563.
6. Henriksson G., Johansson G., Pettersson G. A critical review of cellobiose dehydrogenases. J. Biotechnol. 78:2000a;93-113.
7. Bao W., Usha S.N., Renganathan V. Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white rot fungus Phanerochaete chrysosporium. Arch. Biochem. Biophys. 300:1993;705-713.
8. Li B., Nagalla S.R., Renganathan V. Cellobiose dehydrogenase from Phanerochaete chrysosporium is encoded by two allelic variants. Appl. Environ. Microbiol. 63:1997;796-799.
9. Henriksson G., Zhang L., Li J., Ljungquist P., Reitberger T., Pettersson G.et al. Is cellobiose dehydrogenase from Phanerochaete chrysosporium a lignin degrading enzyme? Biochim. Biophys. Acta. 1480:2000;83-91.
10. Henriksson G., Johansson G., Pettersson G. Is cellobiose oxidase from Phanerochate chrsosporium a one-electron reductase? Biochim. Biophys. Acta. 1144:1993;184-190.
11. Ayers A., Ayers S., Eriksson K.-E.L. Cellobiose oxidase purification and partial characterization of a hemoprotein from Sporotrichum pulverulentum. Eur. J. Biochem. 90:1978;171-181.
12. Samejima M., Ohkubo T., Igarashi K., Isogai A., Kuga S., Sugiyama J.et al. The behaviour of Phanerochaete chrysosporium cellobiose dehydrogenase on adsorption to crystalline and amorphous celluloses. Biotechnol. Appl. Biochem. 25:1997;135-141.
13. Henriksson G., Salumets A., Divne C., Pettersson G. Studies of cellulose binding by cellobiose dehydrogenase and a comparison with cellobiohydrolase I. Biochem. J. 324:1997;833-838.
14. Subramaniam S.S., Nagalla S.R., Renganathan V. Cloning and characterization of a thermostable cellobiose dehydrogenase from Sporotrichum thermophile. Arch. Biochem. Biophys. 365:1999;223-230.
15. Beguin P., Aubert J.P. The biological degradation of cellulose. FEMS Microbiol. Rev. 13:1994;25-58.
16. Henriksson G., Ander P., Pettersson B., Pettersson G. Cellobiose dehydrogenase (cellobiose oxidase) from Phanerochaete chrysosporium as a wood-degrading enzyme. Studies on cellulose, xylan and synthetic lignin. Appl. Microbiol. Biotechnol. 42:1995;790-796.
17. Igarashi K., Samejima M., Eriksson K.-E.L. Cellobiose dehydrogenase enhances Phanerochaete chrysosporium cellobiohydrolase I activity by relieving product inhibition. Eur. J. Biochem. 253:1998;101-106.
18. Tuor U., Wariishi H., Schoemaker H.E., Gold M.H. Oxidation of phenolic arylglycerol beta-aryl ether lignin model compounds by manganese peroxidase from Phanerochaete chrysosporium - oxidative cleavage of an alpha-carbonyl model-compound. Biochemistry. 31:1992;4986-4995.
19. Hilden L., Johansson G., Pettersson G., Li J., Ljungquist P., Henriksson G. Do the extracellular enzymes cellobiose dehydrogenase and manganese peroxidase form a pathway in lignin biodegradation? FEBS Lett. 477:2000;79-83.
20. Temp U., Eggert C. Novel interaction between laccase and cellobiose dehydrogenase during pigment synthesis in the white rot fungus Pycnoporus cinnabarinus. Appl. Environ. Microbiol. 65:1999;389-395.
21. Dumonceaux T., Bartholomew K., Valeanu L., Charles T., Archibald F. Cellobiose dehydrogenase is essential for wood invasion and nonessential for kraft pulp delignification by Trametes versicolor. Enz. Microbial. Tech. 29:2001;478-489.
22. Dumonceaux T.J., Bartholomew K.A., Charles T.C., Moukha S.M., Archibald F.S. Cloning and sequencing of a gene encoding cellobiose dehydrogenase from Trametes versicolor. Gene. 210:1998;211-219.
23. Mouka S.M., Dumonceaux T.J., Record E., Archibald F.S. Cloning and analysis of Pycnoporus cinnabarinus cellobiose dehydrogenase. Gene. 234:1999;23-33.
24. Igarashi K., Verhagen M.F., Samejima M., Schulein M., Eriksson K.-E.L., Nishino T. Cellobiose dehydrogenase from the fungi Phanerochaete chrysosporium and Humicola insolens. A flavohemoprotein from Humicola insolens contains 6-hydroxy-FAD as the dominant active cofactor. J. Biol. Chem. 274:1999;3338-3344.
25. Hallberg B.M., Bergfors T., Backbro K., Pettersson G., Henriksson G., Divne C.A. A new scaffold for binding haem in the cytochrome domain of the extracellular flavocytochrome cellobiose dehydrogenase. Struct. Fold. Design. 8:2000;79-88.
26. Yoshida M., Ohira T., Igarasdhi K., Nagasawa H., Aida K., Hallberg B.M. et al. Production and characterization of recombinant Phanerochaete chrysosporium cellobiose dehydrogenase in the methylotrophic yeast Pichia pastoris. Biosci. Biotechnol. Biochem. 65:2001;2050-2057.
27. Collins P.J., O'Brien M.M., Dobson A.D.W. Cloning and characterization of a cDNA encoding a novel extracellular peroxidase from Trametes versicolor. Appl. Environ. Microbiol. 65:1999;1343-1347.
28. Invitrogen. Multi-copy Pichia expression kit manual, version C. Invitrogen Corporation, Carlsbad, CA, 1998.
29. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1990;403-410.
30. Higgins D.G., Sharp P.M. Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS. 5:1989;151-153.
31. Ausubel FM, Brent R, Kingston E, Moore DD, Seidman JG, Smith JA, et al. Current protocols in molecular biology. New York, NY: Wiley; 1996.
32. O'Callaghan J., O'Brien M.M., McClean K., Dobson A.D.W. Optimization of the expression of a Trametes versicolor laccase gene in Pichia pastoris. J. Ind. Microbiol. Biotechnol. 29:2002;55-59.
33. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
34. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76:1979;4350-4354.
35. Wood J.D., Wood P.M. Evidence that cellobiose:quinone oxidoreductase from Phanerochaete chrysosporium is a breakdown product of cellobiose oxidase. Biochim. Biophys. Acta. 1119:1992;90-96.
36. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
37. Li B., Rotsaert F.A.J., Gold M.H., Renganathan V. Homologous expression of recombinant cellobiose dehydrogenase in Phanerochaete chrysosporium. Biochem. Biophys. Res. Commun. 270:2000;141-146.
38. Cereghino G.P.L., Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24:2000;45-66.
39. Jönnson L., Saloheimo M., Penttil M. Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris. Curr. Genet. 32:1997;425-430.
40. Otterbein L., Record E., Longhi S., Asther M., Moukha S. Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus I-937 and expression in Pichia pastoris. Eur. J. Biochem. 267:2000;1619-1625.
41. Soden D.M., O'Callaghan J., Dobson A.D.W. Molecular cloning of a laccase isozyme gene from Pleurotus sajor-caju and expression in the heterologous Pichia pastoris host. Microbiology. 148:2002;4003-4014.
42. Baminger U., Subramaniam S.S., Renganathan V., Haltrich D. Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii. Appl. Environ. Microbiol. 64:1998;1766-1774.