Fluorescent whole-mount method for visualizing three-dimensional relationships in intact and regenerating adult newt spinal cords

Developmental Dynamics

Volumn 239, Issue 11, 2010, Pages 3048-3057

  Zukor, Katherine A ^a   Kent, David T ^a   Odelberg, Shannon J ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

Confocal imaging; Immunohistofluorescence; Newt; Spinal cord regeneration; Whole mount

Indexed keywords

ANTIBODY; FLUORESCENT DYE; GLUTARALDEHYDE; PARAFORMALDEHYDE; PERIODATE LYSINE PARAFORMALDEHYDE; STREPTAVIDIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; BLEACHING; BONE DEMINERALIZATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EMBEDDING; NEWT; NONHUMAN; PRIORITY JOURNAL; SPINAL CORD; THREE DIMENSIONAL IMAGING; TISSUE REGENERATION;

ANIMALS; FLUORESCENT ANTIBODY TECHNIQUE; MICROSCOPY, CONFOCAL; REGENERATION; SALAMANDRIDAE; SPINAL CORD;

SALAMANDRIDAE;

EID: 77958609701     PISSN: 10588388     EISSN: 10970177     Source Type: Journal    
DOI: 10.1002/dvdy.22441     Document Type: Article

References (16)

1. Becker T, Becker CG. 2001. Regenerating descending axons preferentially reroute to the gray matter in the presence of a general macrophage/microglial reaction caudal to a spinal transection in adult zebrafish. J Comp Neurol 433: 131-147.
2. Becker T, Wullimann MF, Becker CG, Bernhardt RR, Schachner M. 1997. Axonal regrowth after spinal cord transection in adult zebrafish. J Comp Neurol 377: 577-595.
3. Bhairi SM, Mohan CM. 2007. Calbiochem detergents: a guide to the properties and uses of detergents in biology and biochemistry. In: EMD Biosciences, San Diego, CA.
4. Brockes JP. 1997. Amphibian limb regeneration: rebuilding a complex structure. Science 276: 81-87.
5. Davis BM, Duffy MT, Simpson SB Jr. 1989. Bulbospinal and intraspinal connections in normal and regenerated salamander spinal cord. Exp Neurol 103: 41-51.
6. Davis BM, Ayers JL, Koran L, Carlson J, Anderson MC, Simpson SB Jr. 1990. Time course of salamander spinal cord regeneration and recovery of swimming: HRP retrograde pathway tracing and kinematic analysis. Exp Neurol 108: 198-213.
7. Eguchi G, Abe SI, Watanabe K. 1974. Differentiation of lens-like structures from newt iris epithelial cells in vitro. Proc Natl Acad Sci U S A 71: 5052-5056.
8. Ghosh S, Thorogood P, Ferretti P. 1994. Regenerative capability of upper and lower jaws in the newt. Int J Dev Biol 38: 479-490.
9. Iten LE, Bryant SV. 1976. Stages of tail regeneration in the adult newt, Notophthalmus viridescens. J Exp Zool 196: 283-292.
10. Kardon G. 1998. Muscle and tendon morphogenesis in the avian hind limb. Development 125: 4019-4032.
11. Klymkowsky MW, Hanken J. 1991. Whole-mount staining of Xenopus and other vertebrates. Methods Cell Biol 36: 419-441.
12. McLean IW, Nakane PK. 1974. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem 22: 1077-1083.
13. Mitashov VI. 1996. Mechanisms of retina regeneration in urodeles. Int J Dev Biol 40: 833-844.
14. Oberpriller JO, Oberpriller JC. 1974. Response of the adult newt ventricle to injury. J Exp Zool 187: 249-253.
15. Piatt J. 1955. Regeneration of the spinal cord in the salamander. J Exp Zool 129: 177.
16. Rogers S. 1999. Cell biological applications of fluorescence microscopy. In: University of Illinois at Urbana-Champaign, Urbana, IL: Imaging Technology Group, Beckman Institute for Advanced Science and Technology.