Messenger RNA quantification after fluorescence-activated cell sorting using in situ hybridization

Cytometry Part A

Volumn 77, Issue 11, 2010, Pages 1032-1037

  Yamada, Hiroya ^a   Maruo, Rie ^a   Watanabe, Mikio ^a   Hidaka, Yoh ^a   Iwatani, Yoshinori ^a   Takano, Toru ^a  

^a OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Cancer stem cell; FACS mQ; Gene expression profile; In situ hybridization; Stem cell

Indexed keywords

COMPLEMENTARY RNA; IODIDE PEROXIDASE; MESSENGER RNA; THYROGLOBULIN;

ANIMAL; ARTICLE; CANCER STEM CELL; CELL SEPARATION; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE DOSAGE; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PATHOLOGY; RAT; THYROID GLAND; THYROID TUMOR; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; CELL SEPARATION; FLOW CYTOMETRY; GENE DOSAGE; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; IODIDE PEROXIDASE; NEOPLASTIC STEM CELLS; RATS; RNA, COMPLEMENTARY; RNA, MESSENGER; THYROGLOBULIN; THYROID GLAND; THYROID NEOPLASMS;

EID: 77958534282     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20973     Document Type: Article

References (23)

1. Schroeder T. Imaging stem-cell-driven regeneration in mammals. Nature 2008; 453: 345-351.
2. Reya T, Morrison SJ, Clarke MF, Weissman IL. Stem cells, cancer, and cancer stem cells. Nature 2001; 414: 105-111.
3. Takano T. Fetal cell carcinogenesis of the thyroid: Theory and practice. Semin Cancer Biol 2007; 17: 233-240.
4. Takano T, Amino N. Fetal cell carcinogenesis: A new hypothesis for better understanding of thyroid carcinoma. Thyroid 2005; 15: 432-438.
5. O'Brien CA, Pollett A, Gallinger S, Dick JE. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature 2007; 445: 106-110.
6. Ricci-Vitiani L, Lombardi DG, Pilozzi E, Biffoni M, Todaro M, Peschle C, De Maria R. Identification and expansion of human colon-cancer-initiating cells. Nature 2007; 445: 111-115.
7. Klonisch T, Hoang-Vu C, Hombach-Klonisch S. Thyroid stem cells and cancer. Thyroid 2009; 19: 1303-1315.
8. Li C, Heidt DG, Dalerba P, Burant CF, Zhang L, Adsay V, Wicha M, Clarke MF, Simeone DM. Identification of pancreatic cancer stem cells. Cancer Res 2007; 67: 1030-1037.
9. Singh SK, Clarke ID, Terasaki M, Bonn VE, Hawkins C, Squire J, Dirks PB. Identification of a cancer stem cell in human brain tumors. Cancer Res 2003; 63: 5821-5828.
10. Zuba-Surma EK, Kucia M, Ratajczak J, Ratajczak MZ. "Small stem cells" in adult tissues: Very small embryonic-like stem cells stand up! Cytometry Part A 2009; 75A: 4-13.
11. Yoshizaki T, Inaji M, Kouike H, Shimazaki T, Sawamoto K, Ando K, Date I, Kobayashi K, Suhara T, Uchiyama Y, Okano H. Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells. Neurosci Lett 2004; 363: 33-37.
12. Belloc F, Lacombe F, Dumain P, Mergny JL, Lopez F, Bernard P, Reiffers J, Boisseau MR. Flow cytometric estimation of poly(A)+ RNA by fluorescent in situ hybridization. Cytometry 1993; 14: 339-343.
13. Borzi RM, Piacentini A, Monaco MC, Lisignoli G, Degrassi A, Cattini L, Santi S, Facchini A. A fluorescent in situ hybridization method in flow cytometry to detect HIV-1 specific RNA. J Immunol Methods 1996; 193: 167-176.
14. Bayer JA, Bauman JG. Flow cytometric detection of beta-globin mRNA in murine haemopoietic tissues using fluorescent in situ hybridization. Cytometry 1990; 11: 132-143.
15. Robertson KL, Thach DC. LNA flow-FISH: A flow cytometry-fluorescence in situ hybridization method to detect messenger RNA using locked nucleic acid probes. Anal Biochem 2009; 390: 109-114.
16. Takano T, Miyauchi A, Yokozawa T, Matsuzuka F, Maeda I, Kuma K, Amino N. Preoperative diagnosis of thyroid papillary and anaplastic carcinomas by real-time quantitative reverse transcription-polymerase chain reaction of oncofetal fibronectin messenger RNA. Cancer Res 1999; 59: 4542-4545.
17. Takano T, Hasegawa Y, Matsuzuka F, Miyauchi A, Yoshida H, Higashiyama T, Kuma K, Amino N. Gene expression profiles in thyroid carcinomas. Br J Cancer 2000; 83: 1495-1502.
18. Oselin K, Mrozikiewicz PM, Pahkla R, Roots I. Quantitative determination of the human MRP1 and MRP2 mRNA expression in FACS-sorted peripheral blood CD4+. CD8+, CD19+, and CD56+ cells. Eur J Haematol 2003; 71: 119-123.
19. Zito G, Richiusa P, Bommarito A, Carissimi E, Russo L, Coppola A, Zerilli M, Rodolico V, Criscimanna A, Amato M, Pizzolanti G, Galluzzo A, Giordano C. In vitro identification and characterization of CD133 (pos) cancer stem-like cells in anaplastic thyroid carcinoma cell lines. PLoS One 2008; 3: e3544.
20. Yamada H, Maruo R, Watanabe M, Hidaka Y, Iwatani Y, Takano T. Messenger RNA quantification after fluorescence activated cell sorting using intracellular antigens. Biochem Biophys Res Commun 2010; 397: 425-428.
21. Kerstens HM, Poddighe PJ, Hanselaar AG. A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine. J Histochem Cytochem 1995; 43: 347-352.
22. Silahtaroglu AN, Nolting D, Dyrskjot L, Berezikov E, Moller M, Tommerup N, Kauppinen S. Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification. Nat Protoc 2007; 2: 2520-2528.
23. Suzuki K, Lavaroni S, Mori A, Ohta M, Saito J, Pietrarelli M, Singer DS, Kimura S, Katoh R, Kawaoi A, Kohn LD. Autoregulation of thyroid-specific gene transcription by thyroglobulin. Proc Natl Acad Sci USA 1998; 95: 8251-8256.