New ELISA approach based on coiled-coil interactions

Journal of Immunological Methods

Volumn 362, Issue 1-2, 2010, Pages 161-167

  Liberelle, Benoît ^a,b   Bartholin, Laurence ^a   Boucher, Cyril ^a   Murschel, Frédéric ^a   Jolicoeur, Mario ^a,b   Durocher, Yves ^c   Merzouki, Abderrazzak ^b   De Crescenzo, Gregory ^a,b  

^a Bio P Research Unit   (Canada)

^b ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

^c NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

E K coiled coil interactions; EGF; ELISA test

Indexed keywords

EPIDERMAL GROWTH FACTOR; POLYHISTIDINE TAG; PROTEIN ANTIBODY; RECOMBINANT PROTEIN;

ANTIBODY AFFINITY; ANTIBODY DETECTION; ANTIBODY SPECIFICITY; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; MOLECULAR INTERACTION; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; SENSITIVITY ANALYSIS;

ENZYME-LINKED IMMUNOSORBENT ASSAY; EPIDERMAL GROWTH FACTOR; HUMANS; PROTEIN STRUCTURE, SECONDARY; SENSITIVITY AND SPECIFICITY;

EID: 77958498880     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.09.027     Document Type: Article

References (19)

1. Boucher C., St-Laurent G., Loignon M., Jolicoeur M., De Crescenzo G., Durocher Y. The bioactivity and receptor affinity of recombinant tagged EGF designed for tissue engineering applications is defined by the nature and position of the tags. Tissue Eng. A 2008, 14:2069.
2. Boucher C., Liberelle B., Jolicoeur M., Durocher Y., De Crescenzo G. Epidermal growth factor tethered through coiled-coil interactions induces cell surface receptor phosphorylation. Bioconjugate Chem. 2009, 20:1569.
3. Boucher C., St-Laurent G., Jolicoeur M., De Crescenzo G., Durocher Y. Protein detection by Western blot via coiled-coil interactions. Anal. Biochem. 2010, 399:138.
4. Burkhard P., Stetefeld J., Strelkov S.V. Coiled coils: a highly versatile protein folding motif. Trends Cell Biol. 2001, 11:82.
5. Chao H., Bautista D.L., Litowski J., Irvin R.T., Hodges R.S. Use of a heterodimeric coiled-coil system for biosensor application and affinity purification. J. Chromatogr. B. Biomed. Sci. App. 1998, 715:307.
6. Crick F.H.C. The packing of alpha-helices: simple coiled coils. Acta Crystallogr. 1953, 6:689.
7. De Crescenzo G., Litowski J.R., Hodges R.S., O'Connor-McCourt M.D. Real-time monitoring of the interactions of two-stranded de novo designed coiled-coils: Effect of chain length on the kinetic and thermodynamic constants of binding. Biochemistry (Mosc). 2003, 42:1754.
8. De Crescenzo G., Pham P.L., Durocher Y., O'Connor-McCourt M.D. Transforming growth factor-beta (TGF-beta) binding to the extracellular domain of the type II TGF-beta receptor: receptor capture on a biosensor surface using a new coiled-coil capture system demonstrates that avidity contributes significantly to high affinity binding. J. Mol. Biol. 2003, 328:1173.
9. De Crescenzo G., Pham P.L., Durocher Y., Chao H., O'Connor-McCourt M.D. Enhancement of the antagonistic potency of transforming growth factor-beta receptor extracellular domains by coiled coil-induced homo- and heterodimerization. J. Biol. Chem. 2004, 279:26013.
10. Le P.U., Lenferink A.E., Pinard M., Baardsnes J., Massie B., O'Connor-McCourt M.D. Escherichia coli expression and refolding of E/K-coil-tagged EGF generates fully bioactive EGF for diverse applications. Protein Expr. Purif. 2009, 64:108.
11. Lupas A. Coiled coils: new structures and new functions. Trends Biochem. Sci. 1996, 21:375.
12. Mewes H.W., Frishman D., Gruber C., Geier B., Haase D., Kaps A., Lemcke K., Mannhaupt G., Pfeiffer F., Schuller C., Stocker S., Weil B. MIPS: a database for genomes and protein sequences. Nucleic Acids Res. 2000, 28:37.
13. Nakaji-Hirabayashi T., Kato K., Iwata H. Surface-Anchoring of Spontaneously Dimerized Epidermal Growth Factor for Highly Selective Expansion of Neural Stem Cells. Bioconjugate Chem. 2009, 20:102.
14. Noirclerc-Savoye M., Le Gouellec A., Morlot C., Dideberg O., Vernet T., Zapun A. In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae. Mol. Microbiol. 2005, 55:413.
15. Parry D.A., Fraser R.D., Squire J.M. Fifty years of coiled-coils and alpha-helical bundles: a close relationship between sequence and structure. J. Struct. Biol. 2008, 163:258.
16. Pham P.L., Perret S., Cass B., Carpentier E., St-Laurent G., Bisson L., Kamen A., Durocher Y. Transient gene expression in HEK293 cells: peptone addition posttransfection improves recombinant protein synthesis. Biotechnol. Bioeng. 2005, 90:332.
17. Tripet B., Yu L., Bautista D.L., Wong W.Y., Irvin R.T., Hodges R.S. Engineering a de novo-designed coiled-coil heterodimerization domain off the rapid detection, purification and characterization of recombinantly expressed peptides and proteins. Protein Eng. 1996, 9:1029.
18. Tripet B., De Crescenzo G., Grothe S., O'Connor-McCourt M., Hodges R.S. Kinetic analysis of the interactions between troponin C (TnC) and troponin I (TnI) binding peptides: evidence for separate binding sites for the "structural" N-terminus and the "regulatory" C-terminus of TnI on TnC. J. Mol. Recognit. 2003, 16:37.
19. Walshaw J., Woolfson D.N. Socket: a program for identifying and analysing coiled-coil motifs within protein structures. J. Mol. Biol. 2001, 307:1427.