Etoposide, cadmium chloride, benzo[a]pyrene, cyclophosphamide and colchicine tested in the in vitro mammalian cell micronucleus test (MNvit) in the presence and absence of cytokinesis block using L5178Y mouse lymphoma cells and 2-aminoanthracene tested in MNvit in the absence of cytokinesis block using TK6 cells at AstraZeneca UK, in support of OECD draft Test Guideline 487

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 702, Issue 2, 2010, Pages 163-170

  Fellows, Michael D ^a   O'Donovan, Michael R ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

Cytotoxicity; Genotoxicity; In vitro micronucleus

Indexed keywords

2 AMINOANTHRACENE; BENZO[A]PYRENE; CADMIUM CHLORIDE; COLCHICINE; CYCLOPHOSPHAMIDE; ETOPOSIDE;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL DEATH; CYTOKINESIS; CYTOSTASIS; DRUG SCREENING; GENOTOXICITY; LYMPHOMA CELL; MICRONUCLEUS; MICRONUCLEUS TEST; MOUSE; NONHUMAN; PRIORITY JOURNAL; RELATIVE CELL COUNT; RELATIVE INCREASE IN CELL COUNT; RELATIVE POPULATION DOUBLING;

ANIMALS; ANTHRACENES; BENZO(A)PYRENE; CADMIUM CHLORIDE; CELL COUNT; CELL LINE; CELL LINE, TUMOR; CYCLOPHOSPHAMIDE; CYTOKINESIS; CYTOTOXINS; ETOPOSIDE; GREAT BRITAIN; GUIDELINES AS TOPIC; HUMANS; LEUKEMIA L5178; MICE; MICRONUCLEUS TESTS; MUTAGENS;

MAMMALIA;

EID: 77958111830     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2009.09.003     Document Type: Article

References (7)

1. D. Kirkland, Evaluation of Different Cytotoxicity Measures for the In Vitro Micronucleus Test (MNVit): Introduction to the Collaborative Trial, this issue.
2. OECD (Draft), OECD Guideline for Testing of Chemicals. No. 487: In Vitro Mammalian Cell Micronucleus Test (MNvit), available: [http://www.oecd.org/document/55/0,3343, en_2649_34377_2349687_1_1_1_1,00.html].
3. +/- mouse lymphoma mutagenesis assay system. Mutat. Res. 1998, 84:169-181.
4. Lorge E., Hayashi M., Albertini S., Kirkland D. Comparison of different methods for an accurate assessment of cytotoxicity in the in vitro micronucleus test 1. Mutat. Res. 2008, 655:1-3.
5. Fellows M.D., O'Donovan M.R., Lorge E., Kirkland D. Comparison of different methods for an accurate assessment of cytotoxicity in the in vitro micronucleus test. II. Practical aspects with toxic agents. Mutat. Res. 2008, 655:4-21.
6. Fellows M.D., O'Donovan M.R. Cytotoxicity in cultured mammalian cells is a function of the method used to estimate it. Mutagenesis 2007, 22:275-280.
7. Elhajouji A., Cunha M., Kirsch-Volders M. Spindle poisons can induce polyploidy by mitotic slippage and micronucleate mononucleates in the cytokinesis-block assay. Mutagenes 1998, 13:193-198.