Solubilization of Escherichia coli recombinant proteins from inclusion bodies

Cold Spring Harbor Protocols

Volumn 5, Issue 9, 2010, Pages

  Simpson, Richard J ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL CELL; CELL INCLUSION; CENTRIFUGATION; CYTOLYSIS; DISULFIDE BOND; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PRECIPITATION; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN RENATURATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SOLUBILIZATION;

ESCHERICHIA COLI;

EID: 77958099792     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5485     Document Type: Article

References (4)

1. Bollag DM, Rozycki MD, and Edelstein SJ, ed. 1996. Protein methods, 2nd ed. Wiley-Liss, New York.
2. Simpson RJ. 2010. Large-scale extraction of recombinant proteins from bacteria. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5484.
3. Weir MP, Sparks J, Chaplin AM. 1987. Micropreparative purification of recombinant human interleukin-2. J Chromatogr 396: 209-215.
4. Zhang J-G, Moritz RL, Reid GE, Ward LD, Simpson RJ. 1992. Purification and characterization of a recombinant murine interleukin-6: Isolation of N- and C-terminally truncated forms. Eur J Biochem 207: 903-913.