Efficient genomic DNA extraction from low target concentration bacterial cultures using SCODA DNA extraction technology

Cold Spring Harbor Protocols

Volumn 5, Issue 10, 2010, Pages

  So, Austin ^a   Pel, Joel ^a   Rajan, Sweta ^b   Marziali, Andre ^a  

^a Boreal Genomics Inc   (Canada)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOMASS; CONTROLLED STUDY; DNA EXTRACTION; DNA PURIFICATION; ESCHERICHIA COLI; GENE TECHNOLOGY; NONHUMAN; POPULATION ABUNDANCE; PULSED FIELD GEL ELECTROPHORESIS;

BACTERIA (MICROORGANISMS);

EID: 77958097658     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5506     Document Type: Article

References (6)

1. Broemeling DJ, Pel J, Gunn DC, Mai L, Thompson JD, Poon H, Marziali A. 2008. An instrument for automated purification of nucleic acids from contaminated forensic samples. JALA 13: 40-48.
2. Krsek M, Wellington EMH. 1999. Comparison of different methods for the isolation and purification of total community DNA from soil. J Microbiol Methods 39: 1-16.
3. Marziali A, Pel J, Bizzotto D, Whitehead LA. 2005. Novel electrophoresis mechanism based on synchronous alternating drag perturbation. Electrophoresis 26: 82-90.
4. Pel J, Broemeling D, Mai L, Poon HL, Tropini G, Warren RL, Holt RA, Marziali A. 2009. Nonlinear electrophoretic response yields a new parameter for separation of biomolecules. Proc Natl Acad Sci 106: 14796-14801.
5. Pel J, Rajan S, So A, Marziali A. 2010. Efficient genomic DNA extraction from low target concentration E. coli cultures using SCODA DNA extraction technology. Laboratory Focus. http://www.bioscienceworld.ca/January2010.
6. Shaban AM, Haroun BM, Ali MA, Elras MA. 2008. Comparison between conventional membrane filter and PCR methods for detection of coliform, E. coli and Salmonella in drinking water. J Appl Sci Res 4: 1769-1776.