An Instrument for Automated Purification of Nucleic Acids from Contaminated Forensic Samples

JALA - Journal of the Association for Laboratory Automation

Volumn 13, Issue 1, 2008, Pages 40-48

  Broemeling, David J ^a   Pel, Joel ^a   Gunn, Dylan C ^a   Mai, Laura ^a   Thompson, Jason D ^a   Poon, Hiron ^b   Marziali, Andre ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b ROYAL CANADIAN MOUNTED POLICE   (Canada)

Author keywords

DNA purification; extraction; forensic; sample preparation

Indexed keywords

CONCENTRATION (PROCESS); DNA; EXTRACTION; NUCLEIC ACIDS; SUBSTRATES;

AUTOMATED PURIFICATION; DRAG ALTERATION; FORENSIC SAMPLES; SYNCHRONOUS COEFFICIENT;

PURIFICATION;

DNA; DYE; NUCLEIC ACID;

ARTICLE; CLOTHING; COMPUTER INTERFACE; COMPUTER PROGRAM; CONCENTRATION (PARAMETERS); CONTAMINATION; CONTROLLED STUDY; CRIME; DNA EXTRACTION; DNA PURIFICATION; ENZYME MECHANISM; EQUIPMENT DESIGN; FORENSIC PATHOLOGY; LABORATORY AUTOMATION; NUCLEIC ACID ANALYSIS; POLYMERASE CHAIN REACTION; PROTEIN PURIFICATION; SHORT TANDEM REPEAT; SOIL ANALYSIS;

EID: 37549002440     PISSN: 15355535     EISSN: 15402452     Source Type: Journal    
DOI: 10.1016/j.jala.2007.10.008     Document Type: Article

References (10)

1. Improving Service Delivery: The Forensic Science Service. March 28, 2003. Report by the Comptroller and Auditor General, House of Commons. Available at: http://www.nao.gov.uk/publications/nao_reports/02-03/0203523.pdf.
2. Kontanis E.J. Evaluation of real-time PCR amplification efficiencies to detect PCR inhibitors. J. Forensic Sci. 51 (2006) 795-804
3. Wilson I.G. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63 (1997) 3741-3751
4. Lakay F.M., Botha A., and Prior B.A. Comparative analysis of environmental DNA extraction and purification methods from different humic acid-rich soils. J. Appl. Microbiol. 102 (2007) 265-273
5. Larkin A., and Harbison S.A. An improved method for STR analysis of bloodstained denim. Int. J. Legal Med. 112 (1999) 388-390
6. Del Rio S.A., Marino M.A., and Belgrader P. PCR-based human leukocyte antigen (HLA) DQa typing of bloodstained light and dark denim fabric. J. Forensic Sci. 41 (1996) 490-492
7. Eckhart L., Bach J., Ban J., and Tschachler E. Melanin binds reversibly to thermostable DNA polymerase and inhibits its activity. Biochem. Biophys. Res. Commun. 271 3 (2000) 726-730
8. Akane A., Matsubara K., Nakamura H., Takahashi S., and Kimura K. Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J. Forensic Sci. 39 (1994) 362-372
9. Marziali A., Pel J., Bizotto D., and Whitehead L.A. Novel electrophoresis mechanism based on synchronous alternating drag perturbation. Electrophoresis 26 (2005) 82-90
10. Personal Communication, Hiron Poon, Royal Canadian Mounted Police Forensic Laboratory Services, Biology Services, November 23, 2006.