Fixation and preparation of developing tissues from Aedes aegypti

Cold Spring Harbor Protocols

Volumn 5, Issue 10, 2010, Pages

  Clemons, Anthony ^a,b   Haugen, Morgan ^a   Flannery, Ellen ^b   Kast, Kristopher ^b   Jacowski, Caitlin ^a,b   Severson, David ^b   Duman Scheel, Molly ^a,b  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

AEDES AEGYPTI; ANIMAL TISSUE; ARTHROPOD LARVA; ARTICLE; CONTROLLED STUDY; CULTURE TECHNIQUE; DEVELOPMENTAL STAGE; DISSECTION; EGG LAYING; EMBRYO; EMBRYO DEVELOPMENT; GENE EXPRESSION REGULATION; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; INSECT DEVELOPMENT; NONHUMAN; PROTEIN EXPRESSION; PUPA (LIFE CYCLE STAGE); TISSUE FIXATION; TISSUE PREPARATION;

AEDES AEGYPTI;

EID: 77958094466     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5508     Document Type: Article

References (8)

1. Clemons A, Mori A, Haugen M, Severson D, Duman-Scheel M. 2010a. Culturing and egg collection of Aedes aegypti. Cold Spring Harb Protoc (this issue). doi:10.1101/pdb.prot5507.
2. Clemons A, Flannery E, Kast K, Severson D, Duman-Scheel M. 2010b. Immunohistochemical analysis of protein expression during Aedes aegypti development. Cold Spring Harb Protoc (this issue). doi:10.1101/pdb.prot5510.
3. Clemons A, Haugen M, Flannery E, Tomchaney M, Kast K, Jacowski C, Le C, Mori A, Simanton Holland W, Sarro J et al. 2010c. Aedes aegypti: An emerging model for vector mosquito development. Cold Spring Harb Protoc (this issue). doi:10.1101/pdb.emo141.
4. Duman-Scheel M, Clark SM, Grunow ET, Hasley AO, Hill B, Simanton WL. 2007. Delayed onset of midline netrin expression in Artemia franciscana coincides with commissural axon growth and provides evidence for homology of midline cells in distantly related arthropods. Evol Dev 9: 131-140.
5. Haugen M, Tomchaney M, Kast K, Flannery E, Clemons A, Jacowski C, Simanton Holland W, Le C, Severson D, Duman-Scheel M. 2010. Whole-mount in situ hybridization for analysis of gene expression during Aedes aegypti development. Cold Spring Harb Protoc (this issue). doi:10.1101/pdb.prot5509.
6. Patel NH. 1994. Imaging neuronal subsets and other cell types in whole-mount Drosophila embryos and larvae using antibody probes. Methods Cell Biol 44: 445-487.
7. Patel NH, Kornberg TB, Goodman CS. 1989. Expression of engrailed during segmentation in grasshopper and crayfish. Development 107: 201-212.
8. Simanton W, Clark S, Clemons A, Jacowski C, Farrell-Van Zomeren A, Beach P, Browne WE, Duman-Scheel M. 2009. Conservation of arthropod midline netrin accumulation revealed with a cross-reactive antibody provides evidence for midline cell homology. Evol Dev 11: 260-268.