Compound ice-binding site of an antifreeze protein revealed by mutagenesis and fluorescent tagging

Biochemistry

Volumn 49, Issue 42, 2010, Pages 9063-9071

  Garnham, Christopher P ^a   Natarajan, Aditya ^a   Middleton, Adam J ^a   Kuiper, Mike J ^b   Braslavsky, Ido ^c   Davies, Peter L ^a  

^a QUEEN S UNIVERSITY   (Canada)

^b Victorian Parnership for Advanced Computing VPAC   (Australia)

^c OHIO UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; BINDING SITES; BIOCHEMISTRY; FLUORESCENCE; FREEZING; PRISMS; PROTEINS; SEAWATER;

ANTIFREEZE ACTIVITIES; ANTIFREEZE PROTEIN; BINDING SURFACE; FLUORESCENT TAGGING; FREEZING POINT; FUSION PROTEINS; GLOBULAR PROTEINS; GREEN FLUORESCENT PROTEIN; ICE CRYSTALS; ICE GROWTH; ISOFORMS; MULTIGENE FAMILIES; PRISM PLANES; PYRAMIDAL PLANES;

ICE;

ANTIFREEZE PROTEIN; GREEN FLUORESCENT PROTEIN; ICE; ISOPROTEIN; FISH PROTEIN; FLUORESCENT DYE; HYBRID PROTEIN; PROTEIN BINDING;

ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; FLUORESCENCE ANALYSIS; PRIORITY JOURNAL; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PERCIFORMES; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ANTIFREEZE PROTEINS, TYPE III; BINDING SITES; CLONING, MOLECULAR; FISH PROTEINS; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; ICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PERCIFORMES; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 77958093545     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi100516e     Document Type: Article

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