A novel human recombinant single-chain antibody targeting CD166/ALCAM inhibits cancer cell invasion in vitro and in vivo tumour growth

Cancer Immunology, Immunotherapy

Volumn 59, Issue 11, 2010, Pages 1665-1674

  Wiiger, Merete Thune ^a   Gehrken, Hege B ^a   Fodstad, Øystein ^a,b   Mælandsmo, Gunhild M ^a   Andersson, Yvonne ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

ALCAM Matrigel invasion; CD166; In vivo tumour growth; Single chain antibody

Indexed keywords

ACTIVATED LEUKOCYTE CELL ADHESION MOLECULE; ANTINEOPLASTIC AGENT; MATRIGEL; RECOMBINANT SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY 173; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIGEN BINDING; ANTIGEN RECOGNITION; ANTINEOPLASTIC ACTIVITY; ARTICLE; BONE MARROW CELL; BREAST CANCER; CANCER CELL CULTURE; CANCER CONTROL; CANCER INHIBITION; CANCER INVASION; CELL STRAIN HCT116; COLORECTAL CARCINOMA; CONTROLLED STUDY; DRUG PROTEIN BINDING; DRUG SCREENING; DRUG TARGETING; FROZEN SECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL;

ACTIVATED-LEUKOCYTE CELL ADHESION MOLECULE; ANIMALS; COLORECTAL NEOPLASMS; HUMANS; IMMUNOGLOBULIN FRAGMENTS; MICE; MICE, INBRED BALB C; MICE, NUDE; NEOPLASM INVASIVENESS; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES; SURVIVAL RATE;

EID: 77958046455     PISSN: 03407004     EISSN: 14320851     Source Type: Journal    
DOI: 10.1007/s00262-010-0892-3     Document Type: Article

References (37)

1. Nelson AL, Reichert JM (2009) Development trends for therapeutic antibody fragments. Nat Biotechnol 27:331-337
2. Reichert JM (2008) Monoclonal antibodies as innovative therapeutics. Curr Pharm Biotechnol 9:423-130
3. Jain M, Kamal N, Batra SK (2007) Engineering antibodies for clinical applications. Trends Biotechnol 25:307-316
4. Bowen MA, Patel DD, Li X, Modrell B, Malacko AR, Wang WC, Marquardt H, Neubauer M, Pesando JM, Francke U, Haynes BF, Aruffo A (1995) Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med 181:2213-2220
5. van Kempen LCLT, Nelissen JMDT, Degen WGJ, Torensma R, Weidle UH, Bloemers HPJ, Figdor CG, Swart GWM (2001) Molecular basis for the homophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction. J Biol Chem 276:25783-25790
6. Ofori-Acquah SF, King JA (2008) Activated leukocyte cell adhesion molecule: a new paradox in cancer. Transl Res 151:122-128
7. Myklebust AT, Beiske K, Pharo A, Davies CD, Aamdahl S, Fodstad Ø (1991) Selection of anti-SCLC antibodies for diagnosis of bone marrow metastasis. Br J Cancer 63:49-53
8. Fodstad Ø, Brøgger A, Bruland Ø, Solheim OP, Nesland J, Pihl A (1986) Characteristics of a cell line established from a patient with multiple osteosarcoma, appearing 13 years after treatment for bilateral retinoblastoma. Int J Cancer 38:33-40
9. Fodstad Ø , Aamdahl S, Pihl A, Boyd MR (1985) Activity of mitozolomide (NSC 353451), a new imidazoterazine, against xenografts from human melanomas, sarcoma, and lung and colon carcinomas. Cancer Res 45:1778-1786
10. Aamdahl S, Fodstad Ø , Kaalhus O, Pihl A (1986) Chemosensi- tivity profiles of human cancers assessed by the 6-day SRC assay on serially xenografted tumors. Int J Cancer 37:579-587
11. Wen DY, Hall WA, Fodstad Ø (1993) Rapid detection of transferrin receptor expression in glioma cell lines by using magnetic microspheres. Neurosurgery 33:878-881
12. Rye PD, Norum L, Olsen D-R, Garman-Vik S, Kaul S, Fodstad Ø (1996) Brain metastasis model in athymic nude mice using a novel MUC1-secreting human breast-cancer cell line, MA11. Int J Cancer 68:682-687
13. Månsson JE, Fredman P, Bigner DD, Molin K, Rosengren B, Friedman HS, Svennerholm L (1986) Characterization of new gangliosides of the lactotetraose series in murine xenografts of a human glioma cell line. FEBS Lett 201:109-113
14. Løset Ga, Løbersli I, Kavlie A, Stacy JE, Borgen T, Kausmally L, Hvattum E, Simonsen B, Hovda MB, Brekke OH (2005) Construction, evaluation and refinement of a large human antibody phage library based on the IgD and IgM variable gene repertoire. J Immunol Methods 299:47-62
15. Stassar M, Reiersen H (2006) Methods for antibody library screening. International patent application WO 2006/038022
16. Kipriyanov SM, Moldenhauer G, Little M (1997) High level production of soluble single chain antibodies in small-scale Escherichia coli cultures. J Immunol Methods 200:69-77
17. Warren DJ, Bjerner J, Paus E, Bormer OP, Nustad K (2005) Use of an in vivo biotinylated single-chain antibody as capture reagent in an immunometric assay to decrease the incidence of interference from heterophilic antibodies. Clin Chem 51:830-838
18. Swart GWM (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol 81:313-321
19. Pedersen KB, Nesland JM, Fodstad O, Maelandsmo GM (2002) Expression of S100A4, E-cadherin, [alpha]- and [beta]-catenin in breast cancer biopsies. Br J Cancer 87:1281-1286
20. Flatmark K, Mxlandsmo GM, Martinsen M, Rasmussen H, Fodstad Ø (2004) Twelve colorectal cancer cell lines exhibit highly variable growth and metastatic capacities in an orthotopic model in nude mice. Eur J Cancer 40:1593-1598
21. Kurosawa G, Akahori Y, Morita M, Sumitomo M, Sato N, Muramatsu C, Eguchi K, Matsuda K, Takasaki A, Tanaka M, Iba Y, Hamada-Tsutsumi S, Ukai Y, Shiraishi M, Suzuki K, Kurosawa M, Fujiyama S, Takahashi N, Kato R, Mizoguchi Y, Shamoto M, Tsuda H, Sugiura M, Hattori Y, Miyakawa S, Shiroki R, Hoshinaga K, Hayashi N, Sugioka A, Kurosawa Y (2008) Comprehensive screening for antigens overexpressed on carcinomas via isolation of human mAbs that may be therapeutic. Proc Natl Acad Sci 105:7287-7292
22. Piazza T, Cha E, Bongarzone I, Canevari S, Bolognesi A, Polito L, Bargellesi A, Sassi F, Ferrini S, Fabbi M (2005) Internalization and recycling of ALCAM/CD166 detected by a fully human single-chain recombinant antibody. J Cell Sci 118:1515-1525
23. Liu B, Conrad F, Cooperberg MR, Kirpotin DB, Marks JD (2004) Mapping tumor epitope space by direct selection of single-chain Fv antibody libraries on prostate cancer cells. Cancer Res 64:704-710
24. Roth A, Drummond DC, Conrad F, Hayes ME, Kirpotin DB, Benz CC, Marks JD, Liu B (2007) Anti-CD166 single chain antibody-mediated intracellular delivery of liposomal drugs to prostate cancer cells. Mol Cancer Ther 6:2737-2746
25. Ruan W, Sassoon A, An F, Simko JP, Liu B (2006) Identification of clinically significant tumor antigens by selecting phage antibody library on tumor cells in situ using laser capture microdis- section. Mol Cell Proteomics 5:2364-2373
26. van Kilsdonk JWJ, Wilting RH, Bergers M, van Muijen GNP, van Kempen LCLT, Swart GWM (2008) Attenuation of melanoma invasion by a secreted variant of activated leukocyte cell adhesion molecule. Cancer Res 68(10):3671-3679
27. van Kempen LCLT, Meier F, Egeblad M, Kersten-Niessen MJF, Garbe C, Weidle UH, van Muijen GNP, Herlyn M, Bloemers HPJ, Swart GWM (2004) Truncation of activated leukocyte cell adhesion molecule: a gateway to melanoma metastasis. J Investig Dermatol 122:1293-1301
28. Swart GWM, Lunter PC, Kilsdonk JWJ, Kempen LCLT (2005) Activated leukocyte cell adhesion molecule (ALCAM/CD166): signaling at the divide of melanoma cell clustering and cell migration? Cancer Metastasis Rev 24:223-236
29. King J, Ofori-Acquah S, Stevens T, Al-Mehdi AB, Fodstad O, Jiang W (2004) Activated leukocyte cell adhesion molecule in breast cancer: prognostic indicator. Breast Cancer Res 6:R478-R487
30. Burkhardt M, Mayordomo E, Winzer KJ, Fritzsche F, Gansukh T, Pahl S, Weichert W, Denkert C, Guski H, Dietel M, Kristiansen G (2006) Cytoplasmic overexpression of ALCAM is prognostic of disease progression in breast cancer. J Clin Pathol 59:403-409
31. Ihnen M, Muller V, Wirtz RM, Schroder C, Krenkel S, Witzel I, Lisboa BW, Janicke F, Milde-Langosch K (2008) Predictive impact of activated leukocyte cell adhesion molecule (ALCAM/ CD166) in breast cancer. Breast Cancer Res Treat 112:419-427
32. Sawhney M, Matta A, Macha M, Kaur J, DattaGupta S, Shukla N, Ramanathan R (2009) Cytoplasmic accumulation of activated leukocyte cell adhesion molecule is a predictor of disease progression and reduced survival in oral cancer patients. Int J Cancer 124(9):2098-2105
33. Tomita K, van Bokhoven A, Jansen CFJ, Kiemeney LA, Karthaus HFM, Vriesema J, Bussemakers MJG, Witjes JA, Schalken JA (2003) Activated leukocyte cell adhesion molecule (ALCAM) expression is associated with a poor prognosis for bladder cancer patients. Urooncology 3:121-129
34. Mezzanzanica D, Fabbi M, Bagnoli M, Staurengo S, Losa M, Balladore E, Alberti P, Lusa L, Ditto A, Ferrini S, Pierotti MA, Barbareschi M, Pilotti S, Canevari S (2008) Subcellular localization of activated leukocyte cell adhesion molecule is a molecular predictor of survival in ovarian carcinoma patients. Clin Cancer Res 14:1726-1733
35. Kahlert C, Weber H, Mogler C, Bergmann F, Schirmacher P, Kenngott HG, Matterne U, Mollberg N, Rahbari NN, Hinz U, Koch M, Aigner M, Weitz J (2009) Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer 101:457-464.
36. Weichert W, Knosel T, Bellach J, Dietel M, Kristiansen G (2004) ALCAM/CD166 is overexpressed in colorectal carcinoma and correlates with shortened patient survival. J Clin Pathol 57:1160-1164
37. Jezierska A, Olszewski W, Pietruszkiewicz J, Olszewski W, Matysiak W, Motyl T (2006) Activated leukocyte cell adhesion molecule (ALCAM) is associated with suppression of breast cancer cells invasion. Med Sci Monit 12:BR245-BR256