Significance of 'in vivo cryotechnique' for morphofunctional analyses of living animal organs

Journal of Electron Microscopy

Volumn 59, Issue 5, 2010, Pages 395-408

  Ohno, Shinichi ^a   Terada, Nobuo ^a   Ohno, Nobuhiko ^a   Saitoh, Sei ^a   Saitoh, Yurika ^a   Fujii, Yasuhisa ^a  

^a UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

cryobiopsy; freeze substitution; in vivo cryotechnique; living animal organ; morphofunctional study

Indexed keywords

ANIMAL; ANTIBODY SPECIFICITY; BIOPSY; CELL; CELL FUNCTION; CEREBELLUM; CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; CYTOLOGY; EVALUATION; FLUORESCENCE MICROSCOPY; FREEZE DRYING; HUMAN; IMMUNOHISTOCHEMISTRY; LIVER; METHODOLOGY; MOUSE; PHYSIOLOGY; REVIEW; SCANNING ELECTRON MICROSCOPY; SPLEEN; TISSUE FIXATION; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; BIOPSY; CELL PHYSIOLOGICAL PHENOMENA; CELLS; CEREBELLUM; CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; FREEZE SUBSTITUTION; HUMANS; IMMUNOHISTOCHEMISTRY; LIVER; MICE; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE; ORGAN SPECIFICITY; SPLEEN; TISSUE FIXATION;

ANIMALIA;

ANIMALS; CELLS; CYTOLOGY; FREEZING; HISTOLOGY; MORPHOLOGY; PHYSIOLOGY;

CRYOBIOPSY; FREEZE SUBSTITUTION; IMMUNOHISTOCHEMICAL STUDIES; IN VIVO CRYOTECHNIQUE; IN-VIVO; LIVING ANIMAL ORGAN; LIVING ANIMALS; MORPHOFUNCTIONAL STUDY; MORPHOLOGICAL STUDY; PHYSIOLOGICAL PROCESS;

TISSUE;

EID: 77957988084     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfq058     Document Type: Review

References (50)

1. Furukawa T, Ohno S, Oguchi H, Hora K, Tokunaga S, and Furuta S (1991) Morphometric study of glomerular slit diaphragms fixed by rapid-freezing and freeze-substitution. Kidney Int. 40: 621-624.
2. Yoshimura A, Ohno S, Nakano K, Oniki H, Inui K, Ideura T, and Koshikawa S (1991) Three-dimensional ultrastructure of anionic sites of the glomerular basement membrane by a quick-freezing and deep-etching method using a cationic tracer. Histochemistry 96: 107-113.
3. Ohno S, Hora K, Furukawa T, and Oguchi H (1992) Ultrastructural study of the glomerular slit diaphragm in fresh unfixed kidneys by a quick-freezing method. Virchows Arch. B Cell Pathol. 61: 351-358.
4. Yu Y, Leng C G, Kato Y, and Ohno S (1997) Ultrastructural study of glomerular capillary loops at different perfusion pressures as revealed by quick-freezing, freeze-substitution and conventional fixation methods. Nephron 76: 452-459.
5. Yu Y, Leng C G, Kato Y, Terada N, Fujii Y, and Ohno S (1998) Ultrastructural study of anionic sites in glomerular basement membranes at different perfusion pressures by quick-freezing and deep-etching method. Nephron 78: 88-95.
6. Ohno S, Terada N, Fujii Y, Ueda H, and Takayama I (1996) Dynamic structure of glomerular capillary loop as revealed by an in vivo cryotechnique. Virchows Arch. 427: 519-527.
7. Ohno S, Baba T, Terada N, Fujii Y, and Ueda H (1996) Cell biology of kidney glomerulus. Int. Rev. Cytol. 166: 181-230.
8. Ohno N, Terada N, Fujii Y, Baba T, and Ohno S (2004) "In vivo cryotechnique" for paradigm shift to "living morphology" of animal organs. Biomed. Rev. 15: 1-19.
9. Terada N, Ohno N, Li Z, Fujii Y, Baba T, and Ohno S (2006) Application of in vivo cryotechnique to the examination of cells and tissues in living animal organs. Histol. Histopathol. 21: 265-272.
10. Terada N and Ohno S (2004) Immunohistochemical application of cryotechniques to native morphology of cells and tissues. Acta Histochem. Cytochem. 37: 339-345.
11. Chen H B, Yamabayashi S, Ou B, Ohno S, and Tsukahara S (1995) Ultrastructural studies on the corneal superficial epithelium of rats by in vivo cryofixation with freeze substitution. Ophthalmic Res. 27: 286-295.
12. Xue M, Kato Y, Terada N, Fujii Y, Baba T, and Ohno S (1998) Morphological study by an 'in vivo cryotechnique' of the shape of erythrocytes circulating in large blood vessels. J. Anat. 193: 73-79.
13. Zea-Aragon A, Terada N, Ohno N, Fujii Y, Baba T, and Ohno S (2004) Effects of anoxia on serum immunoglobulin and albumin leakage through blood-brain barrier in mouse cerebellum as revealed by cryotechniques. J. Neurosci. Methods 138: 89-95.
14. Ohno N, Terada N, Murata S, Katoh R, and Ohno S (2005) Application of cryotechniques with freeze-substitution for the immunohistochemical demonstration of intranuclear pCREB and chromosome territory. J. Histochem. Cytochem. 53: 55-62.
15. Ohno N, Terada N, and Ohno S (2004) Advanced application of the in vivo cryotechnique to immunohistochemistry for animal organs. Acta Histochem. Cytochem. 37: 357-364.
16. van Harreveld A and Trubatch J (1975) Synaptic changes in frog brain after stimulation with potassium chloride. J. Neurocytol. 4: 33-46.
17. Jehl B, Bauer R, Dorge A, and Rick R (1981) The use of propane/ isopentane mixtures for rapid freezing of biological specimens. J. Microsc. 123: 307-309.
18. Cole R, Matuszek G, See C, and Rieder C L (1990) A simple pneumatic device for plunge-freezing cells grown on electron microscopy grids. J. Electron Microsc. Tech. 16: 167-173.
19. Terada N, Fujii Y, Kato Y, Ueda H, Baba T, and Ohno S (1998) Scanning electron microscopic study of erythrocyte shapes artificially jetted through tubes at different pressures by 'in vitro cryotechnique for erythrocytes'. J. Electron Microsc. 47: 489-493.
20. Terada N and Ohno S (1998) Dynamic morphology of erythrocytes revealed by cryofixation technique. Acta Anatomica Nipponica 73: 587-593.
21. Ohno N, Terada N, and Ohno S (2006) Histochemical analyses of living mouse liver under different hemodynamic conditions by "in vivo cryotechnique". Histochem. Cell Biol. 126: 389-398.
22. Terada N, Ohno N, Saitoh S, and Ohno S (2007) Immunohistochemical detection of hypoxia in mouse liver tissues treated with pimonidazole using "in vivo cryotechnique". Histochem. Cell Biol. 128: 253-261.
23. Saitoh S, Terada N, Ohno N, and Ohno S (2008) Distribution of immunoglobulin- producing cells in immunized mouse spleens revealed with "in vivo cryotechnique". J. Immunol. Methods 331: 114-126.
24. Li Z, Ohno N, Terada N, and Ohno S (2006) Immunolocalization of serum proteins in living mouse glomeruli under various hemodynamic conditions by "in vivo cryotechnique". Histochem. Cell Biol. 126: 399-406.
25. Li Z, Ohno N, Terada N, Zhou D, Yoshimura A, and Ohno S (2006) Application of periodic acid-Schiff fluorescence emission for immunohistochemistry of living mouse renal glomeruli by an "in vivo cryotechnique". Arch. Histol. Cytol. 69: 147-161.
26. Zhou D, Ohno N, Terada N, Li Z, Morita H, Inui K, Yoshimura A, and Ohno S (2007) Immunohistochemical analyses on serum proteins in nephrons of protein-overload mice by "in vivo cryotechnique". Histol. Histopathol. 22: 137-145.
27. Saitoh S, Terada N, Ohno N, Saitoh Y, Soleimani M, and Ohno S (2009) Immunolocalization of phospho-Arg-directed protein kinasesubstrate in hypoxic kidneys using in vivo cryotechnique. Med. Mol. Morphol. 42: 24-31.
28. Hew K W, Heath G L, Jiwa A, and Welsh M J (1993) Cadmium in vivo causes disruption of tight junction-associated microfilaments in rat Sertoli cells. Biol. Reprod. 49: 840-849.
29. Chung N P and Cheng C Y (2001) Is cadmium chloride-induced inter- Sertoli tight junction permeability barrier disruption a suitable in vitro model to study the events of junction disassembly during spermatogenesis in the rat testis? Endocrinology 142: 1878-1888.
30. Liao X, Terada N, Ohno N, Li Z, Fujii Y, Baba T, and Ohno S (2006) Immunohistochemical study of serum albumin in normal and cadmium- treated mouse testis organs by "in vivo cryotechnique". Histol. Histopathol. 21: 35-40.
31. Zhou H, Ohno N, Terada N, Saitoh S, Fujii Y, and Ohno S (2007) Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice revealed by 'in vivo cryotechnique'. Reproduction 134: 307-317.
32. Zhou H, Ohno N, Terada N, Saitoh S, Naito I, and Ohno S (2008) Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique. Reproduction 136: 599-610.
33. Ohno N, Terada N, Saitoh S, and Ohno S (2007) Extracellular space in mouse cerebellar cortex revealed by in vivo cryotechnique. J. Comp. Neurol. 505: 292-301.
34. Terada N, Ohno N, Ohguro H, Li Z, and Ohno S (2006) Immunohistochemical detection of phosphorylated rhodosin in lightexposed retina of living mouse with in vivo cryotechnique. J. Histochem. Cytochem. 54: 479-486.
35. Terada N, Ohno N, Saitoh S, Saitoh Y, and Ohno S (2009) Immunoreactivity of glutamate in mouse retina inner segment of photoreceptors with in vivo cryotechnique. J. Histochem. Cytochem. 57: 883-888.
36. Takayama I, Terada N, Baba T, Ueda H, Fujii Y, Kato Y, and Ohno S (2000) Dynamic ultrastructure of mouse pulmonary alveoli revealed by an in vivo cryotechnique in combination with freeze-substitution. J. Anat. 197: 199-205.
37. Xue M, Baba T, Terada N, Kato Y, Fujii Y, and Ohno S (2001) Morphological study of erythrocyte shapes in red pulp of mouse spleens revealed by an in vivo cryotechnique. Histol. Histopathol. 16: 123-129.
38. Terada N, Ohno N, Li Z, Fujii Y, Baba T, and Ohno S (2005) Detection of injected fluorescence-conjugated IgG in living mouse organs using "in vivo cryotechnique" with freeze-substitution. Microsc. Res. Tech. 66: 173-178.
39. Terada N, Banno Y, Ohno N, Fujii Y, Murate T, Sarna J R, Hawkes R, Zea Z, Baba T, and Ohno S (2004) Compartmentation of the mouse cerebellar cortex by sphingosine kinase. J. Comp. Neurol. 469: 119-127.
40. Murata S, Nakazawa T, Ohno N, Terada N, Iwashina M, Mochizuki K, Kondo T, Nakamura N, Yamane T, Iwasa S, Ohno S, and Katoh R (2007) Conservation and alteration of chromosome territory arrangements in thyroid carcinoma cell nuclei. Thyroid 17: 489-496.
41. Takayama I, Terada N, Baba T, Ueda H, Kato Y, Fujii Y, and Ohno S (1999) "in vivo cryotechnique" in combination with replica immunoelectron microscopy for caveolin in smooth muscle cells. Histochem. Cell Biol. 112: 443-445.
42. Zea-Aragon Z, Terada N, Ohno N, Fujii Y, Baba T, Yoshida M, Ohtsuki K, Ohnishi M, and Ohno S (2004) Replica immunoelectron microscopic study of the upper surface layer in rat mandibular condylar cartilage by a quick-freezing method. Histochem. Cell Biol. 121: 255-259. (Pubitemid 38469745)
43. Terada N, Ohno N, Saitoh S, Fujii Y, Ohguro H, and Ohno S (2007) Raman microscopy of freeze-dried mouse eyeball-slice in conjunction with the "in vivo cryotechnique". Microsc. Res. Tech. 70: 634-639.
44. Terada N, Ohno N, Saitoh S, and Ohno S (2008) Application of "in vivo cryotechnique" to detect erythrocyte oxygen saturation in frozen mouse tissues with confocal Raman cryomicroscopy. J. Struct. Biol. 163: 147-154.
45. Fujii Y, Ohno N, Li Z, Terada N, Baba T, and Ohno S (2006) Morphological and histochemical analyses of living mouse livers by new 'cryobiopsy' technique. J. Electron Microsc. 55: 113-122.
46. Ohno N, Terada N, Bai Y, Saitoh S, Nakazawa T, Nakamura N, Naito I, Fujii Y, Katoh R, and Ohno S (2008) Application of cryobiopsy to morphological and immunohistochemical analyses of xenografted human lung cancer tissues and functional blood vessels. Cancer 113: 1068-1079.
47. Bai Y, Ohno N, Terada N, Saitoh S, Nakazawa T, Nakamura N, Katoh R, and Ohno S (2009) Immunolocalization of serum proteins in xenografted mouse model of human tumor cells by various cryotechniques. Histol. Histopathol. 24: 717-728.
48. Saitoh Y, Terada N, Saitoh S, Ohno N, Fujii Y, and Ohno S (2010) Histochemical approach of cryobiopsy for glycogen distribution in living mouse livers under fasting and local circulation loss conditions. Histochem. Cell Biol. 133: 229-239.
49. Ohno S, Terada N, Ohno N, Fujii Y, and Baba T (2006) "in vivo cryotechnique" for examination of living animal organs, further developing to "cryobiopsy" for humans. Recent Res. Dev. Mol. Cell Biol. 6: 65-90.
50. Ohno N, Terada N, Saitoh S, Zhou H, Fujii Y, and Ohno S (2007) Recent development of in vivo cryotechnique to cryobiopsy for living animals. Histol. Histopathol. 22: 1281-1290.