Ultrastructural study of glomerular capillary loops at different perf usion pressures as revealed by quick-freezing, freeze-substitution and conventional fixation methods

Nephron

Volumn 76, Issue 4, 1997, Pages 452-459

  Yu, Ying ^a   Leng, Chong Guang ^a   Kato, Yasuko ^a   Ohno, Shinichi ^a  

^a UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

Basement membrane; Foot process; Freeze substitution; Quick freezing; Renal glomerulus

Indexed keywords

ANIMAL TISSUE; ARTICLE; CRYOPRESERVATION; FREEZE ETCHING; GLOMERULUS BASEMENT MEMBRANE; GLOMERULUS CAPILLARY; KIDNEY PERFUSION; MALE; MORPHOMETRICS; NONHUMAN; PERFUSION PRESSURE; PRIORITY JOURNAL; RAT; TISSUE FIXATION; ULTRASTRUCTURE;

ANIMALS; BASEMENT MEMBRANE; CAPILLARIES; FREEZE ETCHING; FREEZE SUBSTITUTION; KIDNEY GLOMERULUS; MALE; MICROSCOPY, ELECTRON; PERFUSION; PRESSURE; RATS; RATS, WISTAR; RENAL CIRCULATION; TISSUE FIXATION;

EID: 0030836488     PISSN: 16608151     EISSN: 22353186     Source Type: Journal    
DOI: 10.1159/000190228     Document Type: Article

References (24)

1. Graham RC, Karnovskv MJ: Glomerular permeability, Ultrastructural eytochemical studies using peroxidases as protein tracers, J Exp Med 1966;124:1123-1133.
2. Latia H: The glomerular capillary wall, J Ultra-strucl Res 1970;32:526-544.
3. Ohno S, Hora K, Furukawa T, Oguchi H: Ultrastructural study of the glomerular slit diaphragm in fresh unfixed kidneys by a quick-freezing method, Virchows Arch B Cell Pathol 1992;61:351-358.
4. Yoshimura H: Japanese Handbook of Physiology, Tokyo, Igaku Shoin, 1972. vol 4: Physiology of Metabolism II, Body Fluid, Excretion, pp 320-328.
5. Brenner BM, Baylis C, Deen WM: Transport of molecules across renal glomerular capillaries, Physiol Rev 1976:56:502-534.
6. Ryan GB, Karnovskv MJ: Distribution of endogenous albumin in the rat glomerulus: Role of hemodynamic factors in glomerular barrier function, Kidney Int 1976:9:36-45.
7. Anderson WP, Alcorn D, Gilchrist AI, Whiting JM, Ryan GB: Glomerular actions of ANG II during reduction of renal artery pressure, Am J Physiol 1989;256:FI 021 -FI026.
8. Haley DP, Sarrafian M, Bulger RE, Dobvan DC, Eknoyan G: Structural and functional correlates of effects of angiotensin-induced changes in rat glomerulus, Am J Physiol 1987: 253:F111-F119.
9. Olivetti G, Giacomclli F, Wiener J: Morphometry of superficial glomeruli in acute hypertension in the rat, Kidney Int 1985:27:31-38.
10. Sakai T, Lcmley KV, Hackenthal E, Nagata M, Nobiling R, Kriz W: Changes in glomerular structure following acute mcsangial failure in the isolated perfused kidney, Kidney Int 1992: 41:533-541.
11. Kriz W, Hackenthal E, Nobiling R, Sakai T, Eiger M: A role for podocytes to counteract capillary wall distension, Kidney Int 1994:45: 369-376.
12. de Wardencr HE: The Kidney, An Outline of Normal and Abnormal Structure and Function, Edinburgh, Churchill Livingstone, 1973. pp 107-108.
13. Furukawa T, Ohno S, Oguchi H, Hora K, Tokunaga S, Furuta S: Morphomctric study of glomerular slit diaphragm fixed by rapid-freezing and frcczc-substitution, Kidney Int 1991: 40:621-624.
14. Hora K, Ohno S, Oguchi H, Furukawa T, Furuta S: Three-dimensional study of glomerular slit diaphragm by quick-freezing and deep-etching replica method, Eur J Cell Biol 1990: 53:402-406.
15. Takami H, Naramoto A, Shigematsu H, Ohno S: Ultrastructurc of glomerular basement membrane by quick-freczc and decp-ctch methods, Kidney Int 1991:39:659-664.
16. Chan FL, Inoue S, Ieblond CP: The basement membranes of cryo-fixed or aldehyde-fixed, freeze-substitution tissues are composed of a lamina densa and do not contain a lamina luci-da, Cell Tissue Res 1993:273:41 -52.
17. Hayat MA: Principles and Techniques of Electron Microscopy, Biological Applications, New York, Van Nostrand Reinhold, 1970. vol 1. pp 184-189.
18. Elder HY: Cryofixation; in Bullock GR, Pre-tusz P (eds): Techniques in Immunocytochcm-istry, San Diego, Academic Press, 1989. vol 4. pp 1-28.
19. Adler S: Characterization of glomerular epithelial cell matrix receptors, Am J Pathol 1992; 141:571-578.
20. Rcalc E, Luciano L: The laminae rarae of the glomerular basement membrane, Their manifestation depends on the histochemieal and histological techniques; in Scssa A, Mcroni M, Baltini G (eds): Hereditary Nephritis, Contrib Nephrol, Basel, Karger, 1990. vol 80. pp 32-40.
21. Yoshimura A, Ohno S, Nakano K, Oniki H, Inui K, ideura T, Koshikawa S: Three-dimensional ultrastructure of anionic sites of the glomerular basement membrane by a quick-freezing and deep-etching method using a cationic tracer, Histochemistry 1991;96:107-113.
22. Schnabel E, Anderson JM, Farquhar MG: The tight junction protein ZO-1 is concentrated along slit diaphragms of the glomerular epithelium, J Cell Biol 1990:111:1255-1263.
23. Farquhar MG, Wissig SL, Palade GE: Glomerular permeability, I, Ferritin transfer across the normal glomerular capillary wall, J Exp Med 1961:113:47-66.
24. Rodcwald R, Karnovskv MJ: Porous substructure of the glomerular slit diaphragm in the rat and mouse, J Cell Biol 1974:60:423-433.