Disulfide bond tethering of extracellular loops does not affect the closure of OmpF porin at acidic pH

Proteins: Structure, Function and Bioinformatics

Volumn 78, Issue 14, 2010, Pages 2886-2894

  Wager, Beau ^a   Baslé, Arnaud ^b   Delcour, Anne H ^a  

^a University of Houston   (United States)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Acidic pH; Conformational change; Disulfide bonds; E. coli; Planar lipid bilayer; Porin

Indexed keywords

CYSTEINE; OUTER MEMBRANE PROTEIN F;

ACIDITY; ARTICLE; CONFORMATIONAL TRANSITION; DISULFIDE BOND; ESCHERICHIA COLI; HUMAN; MEMBRANE PERMEABILITY; NONHUMAN; OUTER MEMBRANE; PH MEASUREMENT; PRIORITY JOURNAL;

ACIDS; DISULFIDES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN-ION CONCENTRATION; MICROSCOPY, ATOMIC FORCE; MODELS, MOLECULAR; MUTATION; PORINS; PROTEIN CONFORMATION;

NEGIBACTERIA;

EID: 77957959675     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.22807     Document Type: Article

References (29)

1. Nikaido H. Molecular basis of bacterial outer membrane permeability revisited. Microbiol Mol Biol Rev 2003;67:593-656.
2. Cowan SW, Schirmer T, Rummel G, Steiert M, Ghosh R, Pauptit RA, Jansonius JN, Rosenbusch JP. Crystal structures explain functional properties of two E. coli porins. Nature 1992;358:727-733.
3. Delcour AH. Solute uptake through general porins. Front Biosci 2003;8:d1055-d1071.
4. Delcour AH. Function and modulation of bacterial porins: insights from electrophysiology. FEMS Microbiol Lett 1997;151:115-123.
5. Baslé A, Delcour AH. Modulation of porin function. In: Benz R, Benz R, editors. Structure and function of bacterial and eukaryotic porins. Weinheim: Wiley-Interscience; 2004. pp 79-98.
6. Nestorovich EM, Rostovtseva TK, Bezrukov SM. Residue ionization and ion transport through OmpF channels. Biophys J 2003;85:3718-3729.
7. Schindler H, Rosenbusch JP. Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers. Proc Natl Acad Sci USA 1978;75:3751-3755.
8. Benz R, Janko K, Lauger P. Ionic selectivity of pores formed by the matrix protein (porin) of Escherichia coli.. Biochim Biophys Acta 1979;551:238-247.
9. Buehler LK, Kusumoto S, Zhang H, Rosenbusch JP. Plasticity of Escherichia coli porin channels. Dependence of their conductance on strain and lipid environment J Biol Chem 1991;266:24446-24450.
10. Baslé A, Qutub R, Mehrazin M, Wibbenmeyer J, Delcour AH. Deletions of single extracellular loops affect pH-sensitivity, but not voltage-dependence, of the E. coli porin OmpF. Protein Eng Des Sel 2004; 17:665-672.
11. Todt JC, Rocque WJ, McGroarty EJ. Effects of pH on bacterial porin function. Biochemistry 1992;31:10471-10478.
12. Saint N, Lou KL, Widmer C, Luckey M, Schirmer T, Rosenbusch JP. Structural and functional characterization of OmpF porin mutants selected for larger pore size. II. Functional characterization. J Biol Chem 1996;271:20676-20680.
13. Liu N, Delcour AH. Inhibitory effect of acidic pH on OmpC porin: wild-type and mutant studies. FEBS Lett 1998;434:160-164.
14. Duret G, Simonet V, Delcour AH. Modulation of Vibrio cholerae porin function by acidic pH. Channels 2007;1:70-79.
15. Müller DJ, Engel A. Voltage and pH-induced channel closure of porin OmpF visualized by atomic force microscopy. J Mol Biol 1999;285:1347-1351.
16. Phale PS, Philippsen A, Kiefhaber T, Koebnik R, Phale VP, Schirmer T, Rosenbusch JP. Stability of trimeric OmpF porin: the contributions of the latching loop L2. Biochemistry 1998;37:15663-15670.
17. Karshikoff A, Spassov V, Cowan SW, Ladenstein R, Schirmer T. Electrostatic properties of two porin channels from Escherichia coli. J Mol Biol 1994;240:372-384.
18. Phale PS, Philippsen A, Widmer C, Phale VP, Rosenbusch JP, Schirmer T. Role of charged residues at the OmpF porin channel constriction probed by mutagenesis and simulation. Biochemistry 2001;40:6319-6325.
19. Phale PS, Schirmer T, Prilipov A, Lou KL, Hardmeyer A, Rosenbusch JP. Voltage gating of Escherichia coli porin channels: role of the constriction loop. Proc Natl Acad Sci USA 1997;94:6741-6745.
20. Eppens EF, Saint N, Van Gelder P, van Boxtel R, Tommassen J. Role of the constriction loop in the gating of outer membrane porin PhoE of Escherichia coli. FEBS Lett 1997;415:317-320.
21. Bainbridge G, Mobasheri H, Armstrong GA, Lea EJ, Lakey JH. Voltage-gating of Escherichia coli porin: a cystine-scanning mutagenesis study of loop 3. J Mol Biol 1998;275:171-176.
22. Arbing MA, Dahan D, Boismenu D, Mamer OA, Hanrahan JW, Coulton JW. Charged residues in surface-located loops influence voltage gating of porin from Haemophilus influenzae type b. J Membr Biol 2000;178:185-193.
23. Hazes B, Dijkstra BW. Model building of disulfide bonds in proteins with known three-dimensional structure. Protein Eng 1988;2:119-125.
24. Iyer R, Wu Z, Woster PM, Delcour AH. Molecular basis for the polyamine-OmpF porin interactions: inhibitor and mutant studies. J Mol Biol 2000;297:933-945.
25. Baslé A, Iyer R, Delcour AH. Subconductance states in OmpF gating. Biochim Biophys Acta 2004; 1664:100-107.
26. Montal M, Mueller P. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc Natl Acad Sci USA 1972;69:3561-3566.
27. Cowan SW, Garavito RM, Jansonius JN, Jenkins JA, Karlsson R, Konig N, Pai EF, Pauptit RA, Rizkallah PJ, Rosenbusch JP, Rummel G, Schirmer T. The structure of OmpF porin in a tetragonal crystal form. Structure 1995;3:1041-1050.
28. Iyer R, Delcour AH. Complex inhibition of OmpF and OmpC bacterial porins by polyamines. J Biol Chem 1997;272:18595-18601.
29. Duret G, Delcour AH. Deoxycholic acid blocks Vibrio cholerae OmpT but not OmpU porin. J Biol Chem 2006;281:19899-19905.