Secretory expression and purification of a soluble NADH cytochrome b5 reductase enzyme from Mucor racemosus in Pichia pastoris based on codon usage adaptation

Biotechnology Letters

Volumn 32, Issue 11, 2010, Pages 1705-1711

  Mirzaei, S A ^a   Yazdi, M Tabatabaei ^a   Sepehrizadeh, Z ^a  

^a TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Cytochrome b5 reductase; Mucor racemosus; Protein purification; Relative adaptiveness; Secretory expression

Indexed keywords

ADAPTIVENESS; CYTOCHROME B5; MUCOR RACEMOSUS; PROTEIN PURIFICATION; SECRETORY EXPRESSION;

AMINO ACIDS; ESCHERICHIA COLI; METHANOL;

PURIFICATION;

CYTOCHROME B5 REDUCTASE; FUNGAL DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CODON; CULTURE MEDIUM; DNA SEQUENCE; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; MUCOR; NUCLEOTIDE SEQUENCE; PICHIA; PROMOTER REGION; SECRETION; TIME;

AMINO ACID SEQUENCE; CODON; CULTURE MEDIA; CYTOCHROME-B(5) REDUCTASE; DNA, FUNGAL; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; MUCOR; PICHIA; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TIME FACTORS;

ESCHERICHIA COLI; MUCOR; MUCOR RACEMOSUS; PICHIA PASTORIS;

EID: 77957947581     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0348-z     Document Type: Article

References (15)

1. Asada T, Nagase S, Nishimoto K, Koseki S (2008) Molecular dynamics simulation study on stabilities and reactivities of NADH cytochrome B5 reductase. J Phys Chem B 112: 5718-5727.
2. Asada T, Nagase S, Nishimoto K, Koseki S (2009) Simulation study of interactions and reactivities between NADH cytochrome b5 reductase and cytochrome b5. J Mol Liquids 147: 139-144.
3. Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ (2005) Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa. Biol Reprod 73: 334-342.
4. Bartlett PN (2008) Bioelectrochemistry: fundamentals, Experimental Techniques and Applications, 2nd edn. John Wiley and Sons, New York.
5. Biro JC (2008) Does codon bias have an evolutionary origin? Theoretical Biology and Medical Modelling 5: 1-15.
6. Daly R, Hearn MT (2005) Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J Mol Recognit 18: 119-138.
7. Davis CA, Crowley LJ, Barber MJ (2004) Cytochrome b5 reductase: the roles of the recessive congenital methemoglobinemia mutants P144L, L148P, and R159*. Arch Biochem Biophys 431: 233-244.
8. Hershberg R, Petrov DA (2008) Selection on codon bias. Annu Rev Genet 42: 287-299.
9. Hoffman CS, Winston F (1987) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57: 267-272.
10. Kliman RM, Irving N, Santiago M (2003) Selection conflicts, gene expression, and codon usage trends in yeast. J Mol Evol 57: 98-109.
11. Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270.
12. Nakamura MT, Nara TY (2004) Structure, function, and dietary regulation of delta6, delta5, and delta9 desaturases. Annu Rev Nutr 24: 345-376.
13. Percy MJ, Crowley LJ, Boudreaux J, Barber MJ (2006) Expression of a novel P275L variant of NADH: cytochrome b5 reductase gives functional insight into the conserved motif important for pyridine nucleotide binding. Arch Biochem Biophys 447: 59-67.
14. Sakuradani E, Kobayashi M, Shimizu S (1999) Identification of an NADH-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, Mortierella alpina 1S-4, by sequencing of the encoding cDNA and heterologous expression in a fungus, Aspergillus oryzae. Appl Environ Microbiol 65: 3873-3879.
15. Setayesh N, Sepehrizadeh Z, Jaberi E, Tabatabaei Yazdi M (2009) Cloning, molecular characterization and expression of a cDNA encoding a functional NADH-cytochrome b5 reductase from Mucor racemosus PTCC 5305 in E. coli. Biol Res 42.