Significant human β-cell turnover is limited to the first three decades of life as determined by in vivo thymidine analog incorporation and radiocarbon dating

Journal of Clinical Endocrinology and Metabolism

Volumn 95, Issue 10, 2010, Pages

  Perl, Shira Y ^a,f   Kushner, J A ^b   Buchholz, B A ^c   Meeker, A K ^d   Stein, G M ^b   Hsieh, M ^f   Kirby, M ^e,f   Pechhold, S ^a   Liu, E H ^a   Harlan, D M ^a   Tisdale, J F ^f  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^d DEPARTMENT OF PATHOLOGY   (United States)

^e NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^f NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BROXURIDINE; CARBON 14; CELL DNA; GENOMIC DNA; IDOXURIDINE; INSULIN;

ACCELERATOR MASS SPECTROMETRY; ADULT; AGED; ARTICLE; AUTOPSY; CADAVER; CELL ISOLATION; CELL LABELING; CLINICAL ARTICLE; CONTROLLED STUDY; DEATH; DNA INTEGRATION; DNA PURIFICATION; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VIVO STUDY; LONGEVITY; MALE; MASS SPECTROMETRY; PANCREAS ISLET; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; RADIOMETRIC DATING; SCORING SYSTEM; TURNOVER TIME;

EID: 77957787230     PISSN: 0021972X     EISSN: 0021972X     Source Type: Journal    
DOI: 10.1210/jc.2010-0932     Document Type: Article

References (14)

1. Harlan DM, Kenyon NS, Korsgren O, Roep BO 2009 Current advances and travails in islet transplantation. Diabetes 58:2175-2184
2. Ilic S, Jovanovic L, Wollitzer AO 2000 Is the paradoxical first trimester drop in insulin requirement due to an increase in C-peptide concentration in pregnant type I diabetic women? Diabetologia 43:1329-1330
3. Jovanovic L, Knopp RH, Brown Z, Conley MR, Park E, Mills JL, Metzger BE, Aarons JH, Holmes LB, Simpson JL 2001 Declining insulin requirement in the late first trimester of diabetic pregnancy. Diabetes Care 24:1130-1136
4. Kuroda A, Yamasaki Y, Imagawa A 2003 β-Cell regeneration in a patient with type 1 diabetes mellitus who was receiving immunosuppressive therapy. Ann Intern Med 139:W81
5. Cnop M, Hughes SJ, Igoillo-Esteve M, Hoppa MB, Sayyed F, van de Laar L, Gunter JH, de Koning EJ, Walls GV, Gray DW, Johnson PR, Hansen BC, Morris JF, Pipeleers-Marichal M, Cnop I, Clark A 2010 The long lifespan and low turnover of human islet β cells estimated by mathematical modelling of lipofuscin accumulation. Diabetologia 53:321-330
6. Scharfmann R 2008 Expanding human β cells. Diabetologia 51:692-693
7. Liu EH, Digon 3rd BJ, Hirshberg B, Chang R, Wood BJ, Neeman Z, Kam A, Wesley RA, Polly SM, Hofmann RM, Rother KI, Harlan DM 2009 Pancreatic β cell function persists in many patients with chronic type 1 diabetes, but is not dramatically improved by prolonged immunosuppression and euglycaemia from a β cell allograft. Diabetologia 52:1369-1380
8. Rother KI, Spain LM, Wesley RA, Digon 3rd BJ, Baron A, Chen K, Nelson P, Dosch HM, Palmer JP, Brooks-Worrell B, Ring M, Harlan DM 2009 Effects of exenatide alone and in combination with daclizumab on β-cell function in long-standing type 1 diabetes. Diabetes Care 32:2251-2257
9. Spalding KL, Bhardwaj RD, Buchholz BA, Druid H, Frisén J 2005 Retrospective birth dating of cells in humans. Cell 122:133-143
10. Teta M, Long SY, Wartschow LM, Rankin MM, Kushner JA 2005 Very slow turnover of β-cells in aged adult mice. Diabetes 54:2557-2567
11. Butler AE, Galasso R, Meier JJ, Basu R, Rizza RA, Butler PC 2007 Modestly increased β cell apoptosis but no increased β cell replication in recent-onset type 1 diabetic patients who died of diabetic ketoacidosis. Diabetologia 50:2323-2331
12. Taupin P 2007 BrdU immunohistochemistry for studying adult neurogenesis: paradigms, pitfalls, limitations, and validation. Brain Res Rev 53:198-214
13. Rankin MM, Kushner JA 2009 Adaptive β-cell proliferation is severely restricted with advanced age. Diabetes 58:1365-1372
14. Reimer PJ, Brown TA, Reimer RW 2004 Discussion: reporting and calibration of post-bomb 14C data. Radiocarbon 46:1299-1304