Multiplex real-time qPCR for the detection of Ehrlichia canis and Babesia canis vogeli

Veterinary Parasitology

Volumn 173, Issue 3-4, 2010, Pages 292-299

  Peleg, Ofer ^a   Baneth, Gad ^b   Eyal, Osnat ^b   Inbar, Jacob ^a   Harrus, Shimon ^b  

^a Genaphora LTD   (Israel)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Babesia canis vogeli; Ehrlichia canis; Multiplex qPCR

Indexed keywords

BETA ACTIN; DNA; HEAT SHOCK PROTEIN 70; RNA 16S;

ARTICLE; BABESIA; BABESIA CANIS VOGELI; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISEASE TRANSMISSION; EHRLICHIA CANIS; GENE EXPRESSION; GENE TARGETING; MICROORGANISM DETECTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS;

ANIMALS; BABESIA; BABESIOSIS; DNA, BACTERIAL; DNA, PROTOZOAN; DOG DISEASES; DOGS; EHRLICHIA CANIS; EHRLICHIOSIS; HSP70 HEAT-SHOCK PROTEINS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

BABESIA CANIS VOGELI; EHRLICHIA CANIS; IXODIDA; RHIPICEPHALUS SANGUINEUS;

EID: 77957757745     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2010.06.039     Document Type: Article

References (25)

1. Boivin G., Cote S., Dery P., De Serres G., Bergeron M.G. Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. J. Clin. Microbiol. 2004, 42:45-51.
2. Bustin S.A., Benes V., Garson J.A., Hellemans J., Huggett J., Kubista M., Mueller R., Nolan T., Pfaffl M.W., Shipley G.L., Vandesompele J., Wittwer C.T. The MIQE Guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 2009, 55:611-622.
3. Compston L.I., Sarkobie F., Li C., Candotti D., Opare-Sem O., Allain J.P. Multiplex real-time PCR for the detection and quantification of latent and persistent viral genomes in cellular or plasma blood fractions. J. Virol. Methods 2008, 151:47-54.
4. de Kok J.B., Wiegerinck E.T., Giesendorf B.A., Swinkels D.W. Rapid genotyping of single nucleotide polymorphisms using novel minor groove binding DNA oligonucleotides (MGB probes). Hum. Mutat. 2002, 19:554-559.
5. Doyle C.K., Labruna M.B., Breitschwerdt E.B., Tang Y.W., Corstvet R.E., Hegarty B.C., Bloch K.C., Li P., Walker D.H., McBride J.W. Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene. J. Mol. Diagn. 2005, 7:504-510.
6. Ferrie R.M., Schwarz M.J., Robertson N.H., Vaudin S., Super M., Malone G., Little S. Development, multiplexing, and application of ARMS tests for common mutations in the CFTR gene. Am. J. Hum. Genet. 1992, 51:251-262.
7. Gal A., Harrus S., Arcoh I., Lavy E., Aizenberg I., Mekuzas-Yisaschar Y., Baneth G. Coinfection with multiple tick-borne and intestinal parasites in a 6-week-old dog. Can. Vet. J. 2007, 48:619-622.
8. Heim A., Passos L.M., Ribeiro M.F., Costa-Junior L.M., Bastos C.V., Cabral D.D., Hirzmann J., Pfister K. Detection and molecular characterization of Babesia caballi and Theileria equi isolates from endemic areas of Brazil. Parasitol. Res. 2007, 102:63-68.
9. Higuchi R., Fockler C., Dollinger G., Watson R. Kinetic PCR analysis - real-time monitoring of DNA amplification reactions. Bio-Technology 1993, 11:1026-1030.
10. Jie H.B., Bailey C.W., Ray B.K., Estes D.M., Kumar N., Carson C.A. Single copy Babesia microti hsp70. Mol. Biochem. Parasitol. 1996, 83:241-246.
11. Kim C., Iseki H., Herbas M.S., Yokoyama N., Suzuki H., Xuan X., Fujisaki K., Igarashi I. Development of Taqman-based real-time PCR assays for diagnostic detection of Babesia bovis and Babesia bigemina. Am. J. Trop. Med. Hyg. 2007, 77:837-841.
12. Kledmanee K., Suwanpakdee S., Krajangwong S., Chatsiriwech J., Suksai P., Suwannachat P., Sariya L., Buddhirongawatr R., Charoonrut P., Chaichoun K. Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp and Hepatozoon canis in canine blood. Southeast Asian J. Trop. Med. Public Health 2009, 40:35-39.
13. Kordick S.K., Breitschwerdt E.B., Hegarty B.C., Southwick K.L., Colitz C.M., Hancock S.I., Bradley J.M., Rumbough R., McPherson J.T., MacCormack J.N. Coinfection with multiple tick-borne pathogens in a Walker Hound kennel in North Carolina. J. Clin. Microbiol. 1999, 37:2631-2638.
14. Lehmann L.E., Hunfeld K.P., Emrich T., Haberhausen G., Wissing H., Hoeft A., Stuber F. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med. Microbiol. Immunol. 2007, 197:313-324.
15. Livak K.J., Flood S.J., Marmaro J., Giusti W., Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 1995, 4:357-362.
16. Mullis K.B., Faloona F.A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987, 155:335-350.
17. Nielsen C.B., Singh S.K., Wengel J., Jacobsen J.P. The solution structure of a locked nucleic acid (LNA) hybridized to DNA. J. Biomol. Struct. Dyn. 1999, 17:175-191.
18. Olmeda A.S., Armstrong P.M., Rosenthal B.M., Valladares B., del Castillo A., de Armas F., Miguelez M., Gonzalez A., Rodriguez J.A., Spielman A., Telford S.R. A subtropical case of human babesiosis. Acta Tropica 1997, 67:229-234.
19. Parola P., Roux V., Camicas J.L., Baradji I., Brouqui P., Raoult D. Detection of Ehrlichiae in African ticks by PCR. Trans. R. Soc. Trop. Med. Hyg. 2000, 94:707-708.
20. Peleg O., Baneth G., Eyal O., Inbar J., Harrus S. Use of chimeric DNA-RNA primers in quantitative PCR for detection of Ehrlichia canis and Babesia canis. Appl. Environ. Microbiol. 2009, 75:6393-6398.
21. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29:e45.
22. Sanchez J.A., Pierce K.E., Rice J.E., Wangh L.J. Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis. Proc. Natl. Acad. Sci. U.S.A. 2004, 101:1933-1938.
23. Sirigireddy K.R., Mock D.C., Ganta R.R. Multiplex detection of Ehrlichia and Anaplasma pathogens in vertebrate and tick hosts by real-time RT-PCR. Ann. NY Acad. Sci. 2006, 1078:552-556.
24. Tyagi S., Kramer F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 1996, 14:303-308.
25. van Doorn R., Szemes M., Bonants P., Kowalchuk G.A., Salles J.F., Ortenberg E., Schoen C.D. Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays. BMC Genomics 2007, 8:276.